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OfflineTrippyJuice
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Registered: 03/10/09
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Back at it! Pf tek jars showing myc growth after 4 days z strain *DELETED*
    #23605494 - 09/02/16 05:26 PM (7 years, 4 months ago)

Post deleted by caliko27

Reason for deletion: Idk



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OfflineMushierage
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605511 - 09/02/16 05:31 PM (7 years, 4 months ago)

Looks fine.  Slightly wet but if you were sterile it shouldn't matter. 

Though, are you using a dry verm barrier?  Not seeing one.  If not, it greatly increases the chance for an oopsie.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineTira
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Registered: 11/20/10
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605523 - 09/02/16 05:36 PM (7 years, 4 months ago)

Silicone injection ports are really great, i also use them all the time and they give me great results everytime as long as I remember to wipe the ports with alcohol before injecting.

Is that white circle in the middle of your lid a filter?  Are your jars getting GE?

I didn't do any cakes for some time but the inside of those jars seem kind of wet to me.


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OfflineTira
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Tira]
    #23605527 - 09/02/16 05:38 PM (7 years, 4 months ago)



--------------------
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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Mushierage]
    #23605536 - 09/02/16 05:43 PM (7 years, 4 months ago)

I used a lid tek that's been 100% succesful in the past.  High temp silicone injection ports.  With a tyvek filter gas exchange port in center of lid.  Look at bottom pic. 

I even built a sab to even further my cautiousness to prevent contamination.

I think I was. .9 ml of water over the reccomended water.  Which I was worried about but I tried to overcome that issue by pressure cooking jars for 1.5 hours and letting jars sit in pressure cooker for 2 days before inoculation.  Hoping the water content would drop a bit.


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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605540 - 09/02/16 05:45 PM (7 years, 4 months ago)

Thx for the tek buddy much appreciated. After These jars lm ready to start some bulk grows.


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OfflineMushierage
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605546 - 09/02/16 05:46 PM (7 years, 4 months ago)

I mean, it's not too awful it looks like.  And with the sound of it, you were pretty sterile.  I think it'll be fine.  But next time, use a dry vermiculite barrier.  It'll guarantee any contaminants that may have fallen on the top will not get to the yummy nutrients and lie dormant on the top of the verm until you birth and scrape it off.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Mushierage]
    #23605551 - 09/02/16 05:48 PM (7 years, 4 months ago)

You think I should use dry verm barrier even though I have tyvek filter?


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OfflineMushierage
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605562 - 09/02/16 05:52 PM (7 years, 4 months ago)

Yes.  The Tyvek filter is not a SFD.  If the microscopic holes in it are more than 3 microns, bacteria could get through it.  Not easily, but they could.  A Barrier would guarantee anything that has gotten through and landed on top stays there and doesn't chow down on your project.  It's just an extra sterile safety precaution.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineMushierage
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Mushierage]
    #23605564 - 09/02/16 05:53 PM (7 years, 4 months ago)

And of course I mean not right now.  Lol it's already done.  Just keep in mind for the next one.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineTira
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Registered: 11/20/10
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605569 - 09/02/16 05:54 PM (7 years, 4 months ago)

Having a verm barrier would do no harm.  Actually if you had one it could retain the extra moisture you have in the jar besided its' protective properties (which I don't think you need if you use this kind of lids. But wouldn't hurt to have an extra barrier of protection)


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Tira]
    #23605596 - 09/02/16 06:03 PM (7 years, 4 months ago)

Thx guys for your input. 

Here's more pics from my other glass jars.



Not showing much growth yet.  But a few. Do have something I've never seen.  Not white but it's consiladating with something.


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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605600 - 09/02/16 06:04 PM (7 years, 4 months ago)

Last grow with some csmbodians I used same jars worked like a charm.


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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605607 - 09/02/16 06:07 PM (7 years, 4 months ago)

Above third picture you can see the weird growth the best out of all other pics.


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OfflineTira
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605608 - 09/02/16 06:07 PM (7 years, 4 months ago)

You sure that's not bacteria? Can't tell from the pictures but you should look into it if they look like wet spots to you.


--------------------
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Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Tira]
    #23605617 - 09/02/16 06:09 PM (7 years, 4 months ago)

They definitely look odd..  Time will tell


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OfflineMushierage
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Tira] * 1
    #23605618 - 09/02/16 06:10 PM (7 years, 4 months ago)

Wet spot is a pretty common bacteria.  Unfortunately though it's one of the worst kind you can have, and it usually happens when your jars are too wet. 

Bacillus.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605619 - 09/02/16 06:11 PM (7 years, 4 months ago)

Plus I might of injected a little more spre solution than I'd liked.


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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605625 - 09/02/16 06:12 PM (7 years, 4 months ago)

How fast does this bacteria usually form anybidea?


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OfflineTira
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: TrippyJuice]
    #23605629 - 09/02/16 06:13 PM (7 years, 4 months ago)

Pretty fast.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineMushierage
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Tira]
    #23605639 - 09/02/16 06:16 PM (7 years, 4 months ago)

Once it forms the jar is basically screwed.  You can safely assume nothing will ever colonize.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineTrippyJuice
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Re: Back at it! Pf tek jars showing myc growth after 4 days z strain [Re: Tira]
    #23605640 - 09/02/16 06:16 PM (7 years, 4 months ago)

OK thx I'll keep an eye on that jar that's the only one showing this growth.


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