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VV01313Y
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Registered: 09/02/16
Posts: 16
Last seen: 3 years, 1 month
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WBS Incubation Question
#23605216 - 09/02/16 03:51 PM (7 years, 4 months ago) |
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How do these look? On a first attempt approx 2 weeks into incubation. Tried to shake the jars to better distribute the spores and concerns arose because of how solid the clumps were.






Update -

Time to break them open in a few more days?
Edited by VV01313Y (09/06/16 02:04 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: WBS Incubation Question [Re: VV01313Y]
#23605225 - 09/02/16 03:55 PM (7 years, 4 months ago) |
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Definitely some bacteria going on, that verm in there probably isn't helping with the clumping though. Might still be able to spawn it when it's done colonizing.
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mushboy
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Re: WBS Incubation Question [Re: VV01313Y]
#23605230 - 09/02/16 03:56 PM (7 years, 4 months ago) |
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what tek did you use? i kinda see some verm in the bottom of a jar..?? is that a coffee filter?
growth is growth but clumpy wbs usually leads to bacteria(which i see). you can still use but flush/number of flushes with be affected.
more details plz.
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VV01313Y
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Registered: 09/02/16
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Re: WBS Incubation Question [Re: mushboy]
#23605383 - 09/02/16 04:45 PM (7 years, 4 months ago) |
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I used doc34's however did no have time to soak wbs overnight instead simmered for an hour then put vermiculite at bottom and simmered wbs in after straining for about 15 mins. Two layers of tyvek envelope and cotton ball in lid hole with foil ontop and pressure cooked. Also did not have a lot of time to pressure cook but did it for about 3 hours. After incubation I just slapped two coffee filters over and tied. I'm thinking there might not be enough gas exchange or I did not kill all the bacteria in the wbs due to shortend soak and pressure cook times. Everything else was kept very sterile.
What do you mean by flush/number of flush affected?
Edited by VV01313Y (09/02/16 04:47 PM)
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Mushierage
SWIM Sinker



Registered: 06/29/16
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Re: WBS Incubation Question [Re: VV01313Y]
#23605609 - 09/02/16 06:07 PM (7 years, 4 months ago) |
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Bacteria will almost always reduce yields in comparison to non bacterial spawn. Not always, but probably 99% of the time.
Bacterial can: Cause smaller mushrooms, cause fewer mushrooms, produce abundances of metabolites, stall colonization, force sub to fruit earlier than desired due to colonization brick wall, cause fewer mushroom flushes (which are weekly) and... Wait for it.... Cause your sub to be highly susceptible to molds, which -will- destroy your sub and result in a toss.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: WBS Incubation Question [Re: Mushierage]
#23605615 - 09/02/16 06:08 PM (7 years, 4 months ago) |
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Quote:
Mushierage said: Bacteria will almost always reduce yields in comparison to non bacterial spawn. Not always, but probably 99% of the time.
Bacterial can: Cause smaller mushrooms, cause fewer mushrooms, produce abundances of metabolites, force sub to fruit earlier than desired due to colonization brick wall, cause fewer mushroom flushes (which are weekly) and... Wait for it.... Cause your sub to be highly susceptible to molds, which -will- destroy your sub and result in a toss.
All of this, with the addition that said mold outbreaks can carry over to new grows and if allowed to snowball, will devastate your future chances of success.
Bacteria is not a sign that you've failed completely, but it's an indication that you need to start keeping a closer eye on it for molds.
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mushboy
modboy



Registered: 04/24/05
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Re: WBS Incubation Question [Re: VV01313Y]
#23605690 - 09/02/16 06:31 PM (7 years, 4 months ago) |
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i've found wbs does not like to be simmered.
rinsing at least 6 times(i taste the water) and soaking for 24hr gives a nice loose texture(also a spoon full of gypsum per 7quarts if you can)
Quote:
VV01313Y said: Two layers of tyvek envelope and cotton ball in lid hole with foil ontop and pressure cooked. Also did not have a lot of time to pressure cook but did it for about 3 hours.... ....Everything else was kept very sterile.
What do you mean by flush/number of flush affected?
two layers of tyvek you dont need a cotton ball. did you switched that out for polyfil? . 3hours is a loooong pc time :o you can burn your grain
a flush is the first batch of mushrooms you get from the substrate. however a icky substrate wont give you a nice full flush cause it has bacteria.. it is 'sick'.
Edited by mushboy (09/02/16 06:37 PM)
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