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Offlinespore-ty
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Petri dishes
    #23601884 - 09/01/16 05:04 PM (7 years, 4 months ago)

So what Petris are you guys using?

I want to start getting them but don't know if I should get glass or plastic

Then I don't know what brand...

Anyone use FP' S pyrex dishes or plastic dishes?


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OfflineMushierage
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Re: Petri dishes [Re: spore-ty]
    #23601889 - 09/01/16 05:06 PM (7 years, 4 months ago)

Plastic.    Pyrex is too fucking expensive for something I only use for a week at a time.  Id rather spend my money on grain.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisiblefilthyknees
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Re: Petri dishes [Re: spore-ty]
    #23601897 - 09/01/16 05:09 PM (7 years, 4 months ago)

500 case for like 100$ from a couple vendors, :pm: if you need a link

Lately been using smaller dishes, theyre great, from 500ml agar you get 30 plates instead of 20 with the larger plates.

Especially for isolation/spore germination the smaller plates kick ass.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineKenetic
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Re: Petri dishes [Re: filthyknees]
    #23601950 - 09/01/16 05:24 PM (7 years, 4 months ago)

I use polystyrene 60 mm dishes and 100 mm 3-section petri's.  I'm gonna get regular 100 mm soon too! Then I can really decide.  Everything has it's purpose.








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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleTuhdoww
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Re: Petri dishes [Re: Kenetic]
    #23602030 - 09/01/16 05:55 PM (7 years, 4 months ago)

Quote:

kenetic said:
I use polystyrene 60 mm dishes and 100 mm 3-section petri's.  I'm gonna get regular 100 mm soon too! Then I can really decide.  Everything has it's purpose.










They just come pre sterilized? You just pour them yourself?

I zoomed in on the pic and they're gama sterilized! That's cool


Edited by Tuhdoww (09/01/16 05:56 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Petri dishes [Re: Tuhdoww]
    #23602035 - 09/01/16 05:56 PM (7 years, 4 months ago)



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Offlinespore-ty
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Re: Petri dishes [Re: Kenetic]
    #23602045 - 09/01/16 05:59 PM (7 years, 4 months ago)

Quote:

Mushierage said:
Plastic.    Pyrex is too fucking expensive for something I only use for a week at a time.  Id rather spend my money on grain.





Haha ya just looks nice and oh ya did I mention PYREX one of these days I'll have 100 pyrex dishes mwahaha

Quote:

filthyknees said:
500 case for like 100$ from a couple vendors, :pm: if you need a link

Lately been using smaller dishes, theyre great, from 500ml agar you get 30 plates instead of 20 with the larger plates.

Especially for isolation/spore germination the smaller plates kick ass.





Nice but thanks I got a couple links just wanted to see what people were using but you make a good point about the smaller dishes which I now might consider

Quote:

kenetic said:
I use polystyrene 60 mm dishes and 100 mm 3-section petri's.  I'm gonna get regular 100 mm soon too! Then I can really decide.  Everything has it's purpose.











So on the thirds petri dishes can you do multiple inocs?


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InvisibleTuhdoww
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Re: Petri dishes [Re: bodhisatta]
    #23602062 - 09/01/16 06:02 PM (7 years, 4 months ago)

Quote:

Trusted Cultivator said:
yes

https://www.shroomery.org/forums/showflat.php/Number/21922023



Thanks bodhi.


Definitely gotta get some of those plates.


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OfflineKenetic
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Re: Petri dishes [Re: spore-ty]
    #23602092 - 09/01/16 06:09 PM (7 years, 4 months ago)

That's actually exactly as I do it.  I use parafilm though cause it's so easy to use.  I couldn't get seran wrap to work.
d

Quote:

filthyknees said:


So on the thirds petri dishes can you do multiple inocs?




Yep.  I did 5 3-section petris with a spore sprint and have done several transfers from each section multiple times.  I really like the dishes but they were hard to pour.  That was before I got this:



--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinespore-ty
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Re: Petri dishes [Re: Kenetic]
    #23602456 - 09/01/16 07:41 PM (7 years, 4 months ago)

Fucking nice how much was that? I was literally just looking at some on ebay.. So  I've decided I'm gonna order 2 20 pack sleeves of plastic Petris from FP

Should I sterilize my media in a pc then pour in a sab? Or pour in SAB then sterilize?

Well I suppose the question is are these even autoclaveable? I wrote fp a email but they're closed now,so I'm sure I'll here back from them tomorrow.
so I'll let y'all know, or if anyone has done this I'd love to hear so


Edited by spore-ty (09/01/16 07:49 PM)


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Offlinemushpunx
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Re: Petri dishes [Re: spore-ty]
    #23602648 - 09/01/16 08:34 PM (7 years, 4 months ago)

I really like the Falcon brand. Fungi Perfecti sells it. It has a pull apart sleeve which is easy to work with and has a sterile sleeve folded up inside.

They're really solid, they dont crack like the cheap ones


--------------------

Amateur Mycologists United
AMU Q&A


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OfflineKenetic
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Re: Petri dishes [Re: spore-ty]
    #23602674 - 09/01/16 08:40 PM (7 years, 4 months ago)

You mix the agar up in the media bottle and sterilize it, then pour in the sab.  You can't p.c. polystyrene dishes without melting them.  even if you could they would boil over.

I bought two of those bottles for 30 dollars after shipping.  Not too bad.  I'm still waiting on a wickless alcohol lamp too.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineGreg
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Re: Petri dishes [Re: Kenetic]
    #23602685 - 09/01/16 08:41 PM (7 years, 4 months ago)

I bought one of those wickless lamps and it's been awesome. Burns forever.


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OfflineKenetic
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Re: Petri dishes [Re: Greg]
    #23602689 - 09/01/16 08:43 PM (7 years, 4 months ago)

good to hear!


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleStargate
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Re: Petri dishes [Re: Kenetic]
    #23602761 - 09/01/16 09:05 PM (7 years, 4 months ago)

Personally, I buy these for my lab:
http://cynmar.com/petri-dish-glass/19972-GLASS-PETRI-DISH-60x15mm10PK.html

They are $8.50 for a pack of 10 glass autoclavable petris. So, $0.85 each I use them so many times over and over, they are just more worth it for me in the end. Personally, I use them about 2 times per month each, so they pay themselves off vs disposables in about 10 weeks.


--------------------
From before I started growing gourmet mushrooms:


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Offlinespore-ty
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Re: Petri dishes [Re: Stargate]
    #23602805 - 09/01/16 09:18 PM (7 years, 4 months ago)

Which size media container should I get is 1l okay?

And anybody else use this website?
Quote:

Stargate said:
Personally, I buy these for my lab:
http://cynmar.com/petri-dish-glass/19972-GLASS-PETRI-DISH-60x15mm10PK.html

They are $8.50 for a pack of 10 glass autoclavable petris. So, $0.85 each I use them so many times over and over, they are just more worth it for me in the end. Personally, I use them about 2 times per month each, so they pay themselves off vs disposables in about 10 weeks.




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Offlinemushpunx
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Re: Petri dishes [Re: spore-ty]
    #23602855 - 09/01/16 09:33 PM (7 years, 4 months ago)

1 litre is fine. I usually prep either 500ml or 1 litre batches, a half litre pours a sleeve of 20 plates usually I think.


E rlenmyer flasks , especially with screw tops make great agar media containers now, its all I use.
If you dont have a threaded top on your flask a wad of polyfill or alluminum foil cover works fine!
These are extremely cheap on ebay or Amazon!


--------------------

Amateur Mycologists United
AMU Q&A


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OfflineKenetic
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Re: Petri dishes [Re: mushpunx]
    #23603726 - 09/02/16 05:25 AM (7 years, 4 months ago)

I've got all my dishes from cynmar.  Parafilm too


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinespore-ty
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Re: Petri dishes [Re: Kenetic]
    #23603735 - 09/02/16 05:31 AM (7 years, 4 months ago)



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Offlinespore-ty
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Re: Petri dishes [Re: spore-ty]
    #23603754 - 09/02/16 05:44 AM (7 years, 4 months ago)

Is cling wrap just as effective as parafilm?


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OfflineKenetic
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Re: Petri dishes [Re: spore-ty]
    #23603762 - 09/02/16 05:56 AM (7 years, 4 months ago)

I'd go with any of them.
I tried using cling wrap but it sucked for me.  Parafilm is so easy to use


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Invisibleblindingleaf
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Re: Petri dishes [Re: Kenetic]
    #23603773 - 09/02/16 06:01 AM (7 years, 4 months ago)

they are both effective at what we need them for (sealing dishes from outside contaminants)

i had a hard time with cling wrap too.  but I know a few people who hated using para as well.

start with cling wrap and then next time u make a trade in marketplace, see if some dude won't throw u some para to try  (12" outta be enough to sample it) so u don't have to buy a whole roll and realize u hate it.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineKenetic
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Re: Petri dishes [Re: blindingleaf]
    #23603835 - 09/02/16 06:44 AM (7 years, 4 months ago)

That's a good idea.  I might trade some of my parafilm in the marketplace. 
I could always use more prints


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinespore-ty
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Re: Petri dishes [Re: Kenetic]
    #23603869 - 09/02/16 07:11 AM (7 years, 4 months ago)

Ah ok thanks guys

Kenetic want some b+ prints :smile:


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OfflineBoomertown
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Re: Petri dishes [Re: spore-ty]
    #23603893 - 09/02/16 07:25 AM (7 years, 4 months ago)

DO the covers that come with petris not seal? Is that why everyone uses para-film rather then the covers?


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OfflineKenetic
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Re: Petri dishes [Re: spore-ty]
    #23603895 - 09/02/16 07:27 AM (7 years, 4 months ago)

Nah got plenty of those.  I'll be posting in the marketplace soon.

Lids lift right off


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineThe Mycologist
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Re: Petri dishes [Re: Kenetic]
    #23603900 - 09/02/16 07:30 AM (7 years, 4 months ago)

I like the glass, I can pour them before pressure cooking.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineLeftfield420
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Re: Petri dishes [Re: The Mycologist]
    #23603923 - 09/02/16 07:44 AM (7 years, 4 months ago)

I did that a few times......didn't go very well for me.....so I oven sterilize my pyrex petris wrapped in foil and pc a jar of agar and pour mine in my SAB.....


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Offlinespore-ty
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Re: Petri dishes [Re: Boomertown]
    #23604138 - 09/02/16 09:51 AM (7 years, 4 months ago)

Quote:

Boomertown said:
DO the covers that come with petris not seal? Is that why everyone uses para-film rather then the covers?





Good question I think for ge and as a filter


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InvisibleStargate
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Re: Petri dishes [Re: The Mycologist]
    #23604559 - 09/02/16 12:44 PM (7 years, 4 months ago)

Quote:

The Mycologist said:
I like the glass, I can pour them before pressure cooking.



I do too. I find that I have less contams doing it this way. I only fill them about 1/4-1/3.


--------------------
From before I started growing gourmet mushrooms:


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