Home | Community | Message Board

Avalon Magic Plants
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate, Injection Grain Bag   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Substrate Bags, Substrate Mix

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinekosmokratorshaman
Cosmic Creator
Male User Gallery

Registered: 05/13/15
Posts: 1,206
Last seen: 7 years, 4 months
Sub Question
    #23601200 - 09/01/16 01:37 PM (7 years, 4 months ago)

I have an interesting idea, that I am sure has been addressed before, but I wanted to posit it here. Say I have a monotub. What is to stop me from cutting out the middle portion of mycelium to spawn to sterile grains? Then refilling the hole(s) I removed from my primary sub with sterile grain to also colonize. In my mind, the new grain would provide nutrition to an aging sub, and as long as I never fruited it, and took care, I could keep it contaminant free.

Is this a doable thing? Or am I beating a dead horse?


--------------------
I am.


Extras: Filter Print Post Top
Invisiblefilthyknees
no coincidence
 User Gallery

Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: Sub Question [Re: kosmokratorshaman]
    #23601255 - 09/01/16 01:50 PM (7 years, 4 months ago)

i wouldnt go colonized substrate -> grain jars because innoculant to anything should be made under sterile conditions,
and a colonized substrate would be in the open air.
Reason being, in the sterile jar all the food is being eaten by anything introduced (we hope clean myc.)
but contams would definitly have a foothold in anything nonsterile, making it no good.
Even if heat treatment holds off contams long enough for full colonization there will be some something in the substrate by the time.
If you really want to try this though just sterilize your sub of choice in a spawn bag and spawn your sterile grain master to that, byopsy that and i suppose out of some attempts of colonized sub to grain jar could work. But i would never spend time finding out.
I wouldnt refill a hole in a sub with grain, or cut any sub except
if there are already many pins forming and the contam is not spread widely. But its never ideal.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


Edited by filthyknees (09/01/16 01:54 PM)


Extras: Filter Print Post Top
Offlinekosmokratorshaman
Cosmic Creator
Male User Gallery

Registered: 05/13/15
Posts: 1,206
Last seen: 7 years, 4 months
Re: Sub Question [Re: filthyknees]
    #23601260 - 09/01/16 01:52 PM (7 years, 4 months ago)

It is easy enough just to make more sterile jars to knock up. I was just curious if this was possible/had been done.


--------------------
I am.


Extras: Filter Print Post Top
Invisiblefilthyknees
no coincidence
 User Gallery

Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: Sub Question [Re: kosmokratorshaman]
    #23601271 - 09/01/16 01:56 PM (7 years, 4 months ago)

Made me think :stoned:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


Extras: Filter Print Post Top
OfflineGreg
always learning
I'm a teapot


Folding@home Statistics
Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
Re: Sub Question [Re: kosmokratorshaman]
    #23601299 - 09/01/16 02:04 PM (7 years, 4 months ago)

Just too many vectors for contamination unfortunately.

If you really wanted to use old sub to inoculate jars, you could transfer a tiny bit to a petri dish and isolate away clean growth.
Then you could take that clean growth and inoculate your jars.

Lots of work when you could just go back to the original culture or spores or whatever instead.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate, Injection Grain Bag   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Substrate Bags, Substrate Mix


Similar ThreadsPosterViewsRepliesLast post
* question on substrate mikearch 1,340 7 09/02/02 08:46 AM
by DreaMaTrix
* silly question about substrate minusrestraint 953 4 09/05/02 08:18 AM
by Maveric2112
* Question about Substrate Jars... roykinn 2,297 12 10/19/01 01:31 PM
by Jammer
* The Rookie Begins! A question about substrate. DrD525 1,952 10 01/12/02 09:31 PM
by DrD525
* URGENT question about substrate. greenlight 1,715 8 06/23/02 05:26 AM
by KingBolete
* questions about substrate podman19 2,025 13 04/23/02 08:36 PM
by Roadkill
* Question ABout Substrate? MexicanMushroom 597 1 05/22/02 09:27 AM
by angryshroom
* taking spore print directly on substrate Anonymous 1,562 4 08/05/02 11:09 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
103 topic views. 21 members, 230 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.008 seconds on 14 queries.