|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
kosmokratorshaman
Cosmic Creator


Registered: 05/13/15
Posts: 1,206
Last seen: 7 years, 4 months
|
Sub Question
#23601200 - 09/01/16 01:37 PM (7 years, 4 months ago) |
|
|
I have an interesting idea, that I am sure has been addressed before, but I wanted to posit it here. Say I have a monotub. What is to stop me from cutting out the middle portion of mycelium to spawn to sterile grains? Then refilling the hole(s) I removed from my primary sub with sterile grain to also colonize. In my mind, the new grain would provide nutrition to an aging sub, and as long as I never fruited it, and took care, I could keep it contaminant free.
Is this a doable thing? Or am I beating a dead horse?
-------------------- I am.
|
filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
|
|
i wouldnt go colonized substrate -> grain jars because innoculant to anything should be made under sterile conditions, and a colonized substrate would be in the open air. Reason being, in the sterile jar all the food is being eaten by anything introduced (we hope clean myc.) but contams would definitly have a foothold in anything nonsterile, making it no good. Even if heat treatment holds off contams long enough for full colonization there will be some something in the substrate by the time. If you really want to try this though just sterilize your sub of choice in a spawn bag and spawn your sterile grain master to that, byopsy that and i suppose out of some attempts of colonized sub to grain jar could work. But i would never spend time finding out. I wouldnt refill a hole in a sub with grain, or cut any sub except if there are already many pins forming and the contam is not spread widely. But its never ideal.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
Edited by filthyknees (09/01/16 01:54 PM)
|
kosmokratorshaman
Cosmic Creator


Registered: 05/13/15
Posts: 1,206
Last seen: 7 years, 4 months
|
|
It is easy enough just to make more sterile jars to knock up. I was just curious if this was possible/had been done.
-------------------- I am.
|
filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
|
|
Made me think
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
|
Just too many vectors for contamination unfortunately.
If you really wanted to use old sub to inoculate jars, you could transfer a tiny bit to a petri dish and isolate away clean growth. Then you could take that clean growth and inoculate your jars.
Lots of work when you could just go back to the original culture or spores or whatever instead.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 103 topic views. 21 members, 230 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|