|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
catsfred7
Stranger
Registered: 09/01/16
Posts: 17
Last seen: 6 years, 11 months
|
Did not shake rye seed jars before inoculation?
#23600856 - 09/01/16 11:43 AM (7 years, 4 months ago) |
|
|
I did not shake my rye seed jars after sterilization in PC.
I inoculuated these jars with out being shaken and went into a black bag inside my rubbermaid incubator.
I have thermostat set to 86F.
I made injection holes to small for needle. So I am unsure of the amount of liquid that went into each jar ,as some liquid came up on the lid.
--------------------------------
I read that jars must be shaken before inculation. Also read 86F may be too hot for inoculation?
Did I mess up my jars? Is there hope? Should I shake the Jars now 24 hours after inoculation? I was going to wait to day 3.
I saw 86F some where but now reading it should be lower for inoculatiion. Whats the truth?
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
|
Re: Did not shake rye seed jars before inoculation? [Re: catsfred7]
#23600884 - 09/01/16 11:52 AM (7 years, 4 months ago) |
|
|
86F will not kill the spores or anything, but it promotes the growth of contaminants. So take them out of the incubator or if you really need heat turn it down. How warm is your room?
I am unsure how you inoculated or what you did, you said the holes were too small. I don't know what you mean about "some liquid came up on the lid."
|
BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
|
Re: Did not shake rye seed jars before inoculation? [Re: blackout]
#23600978 - 09/01/16 12:25 PM (7 years, 4 months ago) |
|
|
not shaking is probably fine thats to spread out water but they are never clumped together for me tho so idk if yours is clumbed, id toss them if they did.
how the hell did u make holes smallet than the needle? did u use RTV for the injection holes or what?i think a jar i had that didnt grow was due to me not going deep enough once( i put the RTv real thick on the bottom of the lid on my ports) so now i look at the needle tip thru the glass
ditch the incubator do a natural room temp 70-78(74-78 top of closet for me)
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
Edited by BaronVonBud (09/01/16 12:28 PM)
|
catsfred7
Stranger
Registered: 09/01/16
Posts: 17
Last seen: 6 years, 11 months
|
Re: Did not shake rye seed jars before inoculation? [Re: BaronVonBud]
#23601058 - 09/01/16 12:48 PM (7 years, 4 months ago) |
|
|
I turned down my incubator to 80F.
Natural room temps are 65F to 80F.
I did a test with a jar that I did not inoculate and I shook it once. It took quite a few shakes to get bottom loose.
I'm sure I got spores into every jar even those the needle was Bigger than the hole. I guess only time will tell now.
The needle was huge as shit I wish I looked at the size of needle before Making holes in lid.
|
kosmokratorshaman
Cosmic Creator


Registered: 05/13/15
Posts: 1,206
Last seen: 7 years, 4 months
|
Re: Did not shake rye seed jars before inoculation? [Re: catsfred7]
#23601215 - 09/01/16 01:39 PM (7 years, 4 months ago) |
|
|
I think that you might have botched your jars. Just squirting the spore solution onto and around the holes in the lid does not guarantee it will drain through the small holes into your grain. Also, wouldn't this greatly advance the chance for contaminants?
-------------------- I am.
|
Tuhdoww
Sub Slapper


Registered: 08/23/16
Posts: 300
|
|
Shake them up dude. I'd rather have the water off the sides and slow conization. Then wet grains up against the glass contaminating
|
Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
|
Re: Did not shake rye seed jars before inoculation? [Re: Tuhdoww]
#23601428 - 09/01/16 02:51 PM (7 years, 4 months ago) |
|
|
Quote:
Tuhdoww said: Shake them up dude. I'd rather have the water off the sides and slow conization. Then wet grains up against the glass contaminating
This
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Did not shake rye seed jars before inoculation? [Re: catsfred7]
#23601687 - 09/01/16 04:14 PM (7 years, 4 months ago) |
|
|
Quote:
catsfred7 said: Natural room temps are 65F to 80F.
where do you live? 15degree swing?
colonizing falls off sharply at 81.(i think RR said that) sooo leave them at room temp.
|
catsfred7
Stranger
Registered: 09/01/16
Posts: 17
Last seen: 6 years, 11 months
|
Re: Did not shake rye seed jars before inoculation? [Re: mushboy]
#23602192 - 09/01/16 06:34 PM (7 years, 4 months ago) |
|
|
Quote:
mushboy said:
Quote:
catsfred7 said: Natural room temps are 65F to 80F.
where do you live? 15degree swing?
colonizing falls off sharply at 81.(i think RR said that) sooo leave them at room temp.
colombia. im leaving them in the incubator at 80F. it gets pretty cold at night where im at.
|
Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
|
Re: Did not shake rye seed jars before inoculation? [Re: catsfred7] 2
#23602240 - 09/01/16 06:44 PM (7 years, 4 months ago) |
|
|
Tips:
Don't put spores to grain. It's dirty as hell and almost always gives you some type of bacteria that effects yields. Use spore>agar>grain and then g2g if you need/want.
Get that temp down, like way down. Like to 73F down. 86F is prime conditions for anaerobic bacteria and molds that will end in a jar toss. Lower 70s makes it hard for contaminates to thrive, and with the way you inoculated, you're going to need it.
Prepare your jars properly prior to PC. Why go through all that trouble if you're just going to botch the sterile tek or inoculation?
And finally, if you do happen to ever use spore to grain again, which I do not suggest doing, don't shake it after inoculation. Shoot it toward the sides of the jar and let it go. Shake at 30% and don't shake again till its done and you're using it as spawn.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
|
catsfred7
Stranger
Registered: 09/01/16
Posts: 17
Last seen: 6 years, 11 months
|
Re: Did not shake rye seed jars before inoculation? [Re: Mushierage]
#23602341 - 09/01/16 07:10 PM (7 years, 4 months ago) |
|
|
So should I shake now or not shake?
Lots of information is mis leading off line >.>
|
BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
|
Re: Did not shake rye seed jars before inoculation? [Re: catsfred7]
#23604147 - 09/02/16 09:55 AM (7 years, 4 months ago) |
|
|
U made self healing injection sites tho right? Idk about shaking them now it might harm the young myc and you'll be shaking at 30% anyway My bird seed never clumps together. I use Frank's teks
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
Edited by BaronVonBud (09/02/16 09:57 AM)
|
BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
|
Re: Did not shake rye seed jars before inoculation? [Re: BaronVonBud]
#23604166 - 09/02/16 10:04 AM (7 years, 4 months ago) |
|
|
If your house is 65 at night I bet a high up cabinet, over the fridge, or the top of your closet is 5-10 degrees warmer
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
|
catsfred7
Stranger
Registered: 09/01/16
Posts: 17
Last seen: 6 years, 11 months
|
Re: Did not shake rye seed jars before inoculation? [Re: BaronVonBud]
#23604293 - 09/02/16 10:58 AM (7 years, 4 months ago) |
|
|
Quote:
BaronVonBud said: U made self healing injection sites tho right? Idk about shaking them now it might harm the young myc and you'll be shaking at 30% anyway My bird seed never clumps together. I use Frank's teks
no i just made holes with a nail and a layer covered by tin foil, i decided not to shake and lowered the incubator to be at a steady 73 F.
I will take pictures if anything happens.
|
BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
|
Re: Did not shake rye seed jars before inoculation? [Re: catsfred7]
#23604808 - 09/02/16 02:18 PM (7 years, 4 months ago) |
|
|
You need to buy high temp silicon for the self healing ports RTV silicon is great it's used for head gaskets on automobiles so any car parts store will have it I don't think it's gonna work with open holes covered with foil but it might if u used a still air box and covered them inside it
You'll need self healing injection sites and a gas exchange hole covered in tyvek or something Without those I'd google how to bulk spawn with PFtek
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
Edited by BaronVonBud (09/02/16 02:22 PM)
|
BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
|
Re: Did not shake rye seed jars before inoculation? [Re: BaronVonBud]
#23604836 - 09/02/16 02:26 PM (7 years, 4 months ago) |
|
|
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
|
|