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TheLongComma
Ouch My Balls


Registered: 08/07/16
Posts: 53
Last seen: 3 days, 16 hours
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Petri dishes compromised?
#23600001 - 09/01/16 05:01 AM (7 years, 5 months ago) |
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Dishes arrived with a few small holes/rips in the sleeve plastic. In my mind they're no longer sterile... has anyone else run into this issue? Did you attempt to use them anyway?
I noticed the sleeve plastic seemed thin and weak anyway - let me know reliable vendors to use as ideally I would initially like to use petris for MS/clone grow outs and then xfer clean growth to pasty plates.
-------------------- Purdy Agar Tek
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 2 months
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They are probably fine actually. They seal rather well. Just wipe the outsides done with alcohol, put into a still air box, and inoculate them.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 24 days, 6 hours
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Re: Petri dishes compromised? [Re: Mushierage]
#23600044 - 09/01/16 05:38 AM (7 years, 5 months ago) |
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. Disposable plates are never packed very tight in the sleeve, the lids and the dish separate from each other quite easily in the package. If there are holes in the sleeve they could contaminate very easily.
You can go ahead an pour them if you like but I would let them sit a couple weeks at room temp go make sure they are clean and nothing grows on them.
I buy my plates off Fungi Perfecti because I really like the "Falcon" brand plates, they are heavy duty, have a tear away sleeve which is easy to open and come with a sterile sleeve folded up to pack them in after pouring.
--------------------
 Amateur Mycologists United AMU Q&A
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Petri dishes compromised? [Re: mushpunx]
#23600053 - 09/01/16 05:45 AM (7 years, 5 months ago) |
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i won't use it if there is legit rip or tear but if its a pinhole or two, yes i will pour the sleeve, and watch/mark it like mushpunx said. just wait 4-5 days. it will show in that time if its there.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 24 days, 6 hours
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2 weeks is kinda overkill haha
--------------------
 Amateur Mycologists United AMU Q&A
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 4 months, 3 days
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Re: Petri dishes compromised? [Re: mushpunx]
#23600205 - 09/01/16 07:39 AM (7 years, 5 months ago) |
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I bought a sleeve of 20 on ebay for cheap and the pack was ripped, never used em.
On the other hand I did accidentally open a sleeve outside the sab recently, they haven't contamed yet.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 11 months
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Re: Petri dishes compromised? [Re: Greg]
#23600215 - 09/01/16 07:43 AM (7 years, 5 months ago) |
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So do you guys use plastic plates or only glass?
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 4 months, 3 days
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Re: Petri dishes compromised? [Re: spore-ty]
#23600232 - 09/01/16 07:54 AM (7 years, 5 months ago) |
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I use plastic. I used to do no-pour in glass jars with polyfill but I had a ton of issue with them so I switched and it's been smooth sailing since.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 11 months
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Re: Petri dishes compromised? [Re: Greg]
#23600276 - 09/01/16 08:22 AM (7 years, 5 months ago) |
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So do you buy them pre poured now?
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Petri dishes compromised? [Re: Greg]
#23600285 - 09/01/16 08:28 AM (7 years, 5 months ago) |
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no one who works with agar consistently buys pre poured plates.
glass plates can be pricey and slippery with iso. I store plates a lot, so I would need even more if I switched to glass. if I found 60 for a good price, I might think about it.
no pour pasty plates and glass half pints are a cheaper option, but need more space for storing. its not necessarily hard to see thru them, but its tricky to get a good picture without opening the lid.
Quote:
Greg said: I use plastic. I used to do no-pour in glass jars with polyfill but I had a ton of issue with them so I switched and it's been smooth sailing since.

^^nice wrap & label
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 4 months, 3 days
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Quote:
spore-ty said: So do you buy them pre poured now?
Nah, I pour agar in my SAB.
I use these old sake bottles modified with SFDs for pressure cooking the agar.
 
Quote:
blindingleaf said: ^^nice wrap & label
Thanks! I used to wrap plates horribly but eventually I realized I was doing it wrong and watched a video on how to wrap them properly.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 4 months
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Re: Petri dishes compromised? [Re: Greg]
#23600679 - 09/01/16 10:33 AM (7 years, 5 months ago) |
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I love parafilm. Nice saki bottles too! I believe I saw you post that somewhere before. I just bought a couple pyrex media bottles 500ml and they are designed really well. Pouring in a San is a breeze now
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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