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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Tampanesis Luck?
#23600066 - 09/01/16 05:58 AM (7 years, 4 months ago) |
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After years of trying my luck with p. Tamp, I think I have something that looks like growth where I inoculated and a brown blob appearing in the middle? Does this look like a stone forming on the plate or more like a contam?
This second one is just a transfer from agar again. Does this look right?

Thanks
-------------------- Why reinvent the wheel when someone here has already done the science?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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tamp mycelium is pure white, so no, thats probably cinnamon mold.
hard to see anything in the 2nd pic its too blurry
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Sorry, here is a sharper picture:
-------------------- Why reinvent the wheel when someone here has already done the science?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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that looks a bit more normal. tamp usually has a whispy characteristic to it.
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Okay, thanks for the feedback. Do you reckon transfer it to a plate that has coffee added for faster colonisation or do you think a low ambient temperature of 20DegC (68F) would be the main bottleneck in my growth speed at the moment?
Cheers
-------------------- Why reinvent the wheel when someone here has already done the science?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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I wouldn't add coffee (ever, to anything). Just let it do its thing, it's fine. 68 degrees is fine too. Just treat it like u would a cube - they originate from the same climate. It usually takes a pasty plate around 2 weeks to reach the edge of the plate.
Edited by Munchauzen (09/01/16 09:16 AM)
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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I use 10% coffee in agar for sclerotia formation stimulation and when soaking grain for sclerotia(~25-50%). it is worth it.
Guys who have grown a lot of stones like devilstone, stonesun, rr, ect have led me to use it, and i found this info in my refrence journal growing stones
Edited by filthyknees (09/01/16 02:08 PM)
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 19 hours
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What are you trying to do exactly? Just germinate and clean up a culture to inoculate grain masters with?
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 Amateur Mycologists United AMU Q&A
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Re: Tampanesis Luck? [Re: mushpunx]
#23602558 - 09/01/16 08:12 PM (7 years, 4 months ago) |
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Sure, that's exactly right. I have heard that the stones can be cultured a bit prior to planting a wedge, although given natural strain and spore variance, this difference should only be small in the bigger scheme? That is; the stone output per strain isolation should not vary a huge deal.
-------------------- Why reinvent the wheel when someone here has already done the science?
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 19 hours
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Quote:
AgarStudent said: Sure, that's exactly right. I have heard that the stones can be cultured a bit prior to planting a wedge, although given natural strain and spore variance, this difference should only be small in the bigger scheme? That is; the stone output per strain isolation should not vary a huge deal.
Sure if you get a stone in a plate you can clone it
If you isolate strains though you wont know untill you fruit them out tho
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 Amateur Mycologists United AMU Q&A
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Quote:
Munchauzen said: I wouldn't add coffee (ever, to anything).
Why's that?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Tampanesis Luck? [Re: YaMoonSun]
#23602692 - 09/01/16 08:44 PM (7 years, 4 months ago) |
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Simple is my style. I'm the lazy cultivator. All my teks and methods are aimed at being the easiest, simplest method with the least amount of vectors. I simply don't use coffee because it isn't necessary. I've grown plenty of tamps and mexicana without additives and they do great
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Fair enough
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Tampanesis Luck? [Re: YaMoonSun]
#23602728 - 09/01/16 08:55 PM (7 years, 4 months ago) |
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Never posted in the tamp thread awhile back, my bad Munch.
But, my tubs stalled out and I never got to see fruits. I believe I over-saturated my cvg and that's why I didn't get good results.
Such a noobish mistake, I know, it was surprising for me too. From now on, I've been using 5.5 quarts boiling water for each 650g coir brick. Perfect field capacity every time.
Can't believe it took me this long to realize most of my substrates have been on the wet side. This is soo embarrasing lol
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Quote:
Munchauzen said: Simple is my style. I'm the lazy cultivator. All my teks and methods are aimed at being the easiest, simplest method with the least amount of vectors. I simply don't use coffee because it isn't necessary. I've grown plenty of tamps and mexicana without additives and they do great 
Just because it's not necessary doesn't mean it's not worth it. You wanna be lazy? Maximize yields and do less tubs. That's lazy for you.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Tampanesis Luck? [Re: 36fuckin5]
#23604149 - 09/02/16 09:57 AM (7 years, 4 months ago) |
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Quote:
36fuckin5 said:
Quote:
Munchauzen said: Simple is my style. I'm the lazy cultivator. All my teks and methods are aimed at being the easiest, simplest method with the least amount of vectors. I simply don't use coffee because it isn't necessary. I've grown plenty of tamps and mexicana without additives and they do great 
Just because it's not necessary doesn't mean it's not worth it. You wanna be lazy? Maximize yields and do less tubs. That's lazy for you.
You wont get an argument out of me and you don't have to agree, like I said it's my style to not use additives in any grows. Compared to using coffee my yields are completely acceptable and when giving advice to beginners i really prefer simplicity. I also don't make tubs from stone producers anyway cause that would anti-lazy. I keep that shit in jars.
Edited by Munchauzen (09/02/16 10:29 AM)
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