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OfflineAgarStudent
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Are these grains too wet?
    #23600059 - 09/01/16 05:53 AM (7 years, 4 months ago)

Hi,

Previously had a lot of trouble getting proper colonisation of mexicana truffles, as my grains were too dry, resulting in colonisation starting and then stopping and curling up.
I went the extra effort to ensure that the grains were properly hydrated this time by bringing to the boil after taking out of the jars.

My only question is, Do you think they are not too wet? The grains are now puffed out (and they were not before).  One one jar that I seems to have had success with had the grains puffed out.

Thanks for feedback :smile:



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Invisibleblindingleaf
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Re: Are these grains too wet? [Re: AgarStudent]
    #23600063 - 09/01/16 05:57 AM (7 years, 4 months ago)

yes.  if u didn't PC those yet, they will burst during the cycle IME

u can have some pooling around the grains before PCing but only if they ARENT already completely hydrated.  those are completely saturated, and the excess water will cause them to burst


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OfflineAgarStudent
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Re: Are these grains too wet? [Re: blindingleaf]
    #23600067 - 09/01/16 06:00 AM (7 years, 4 months ago)

Would you recommend dumping them or do you think they would still colonise properly?


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OfflineAgarStudent
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Re: Are these grains too wet? [Re: AgarStudent]
    #23600079 - 09/01/16 06:11 AM (7 years, 4 months ago)

This is another one from the same batch -
Not quite as soaking, but is this doable?



If not, then is RR's method of soaking for 24 hours and then bringing to the boil for ten minutes a pretty reliable method for getting the water levels perfect?

Never seem to have had this issue before with cubes, but mexicana is much more fussy!

Cheers


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Invisibleblindingleaf
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Re: Are these grains too wet? [Re: AgarStudent]
    #23600085 - 09/01/16 06:17 AM (7 years, 4 months ago)

i mean if it worked for cubes maybe it will work for mex.  I don't prep my grains any different for any species tho.

I dont boil after a soak.  usually I pull them out right as the simmering starts. I'm looking for a small amount of un-hydrated innards to be left, "al dente" kinda

I dont wanna ya to dump them cause thats a waste and what if they work?  can u just use them for cubes if they worked before and prep new grains for mex a little drier?


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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OfflineThe Mycologist
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Re: Are these grains too wet? [Re: blindingleaf]
    #23600116 - 09/01/16 06:48 AM (7 years, 4 months ago)

I believe RR keeps them a little on the dry side (he wrote that somewhere). The hydration lies within the berries. You dont have to worry so much about their outsides being wet.


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OfflineAgarStudent
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Re: Are these grains too wet? [Re: The Mycologist]
    #23600132 - 09/01/16 07:02 AM (7 years, 4 months ago)

Ah yes I recall him mentioning that in a post -
After trying it out there seems such a fine line between a bit on the dry side and too dry.
It is not as bad with grain-to-grain transfers, but it seems that if an agar wedge has not got some moisture to bite onto, then it seems to shrink and gives up the ghost.


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Invisibleblindingleaf
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Re: Are these grains too wet? [Re: AgarStudent]
    #23600142 - 09/01/16 07:08 AM (7 years, 4 months ago)

Quote:

It is not as bad with grain-to-grain transfers, but it seems that if an agar wedge has not got some moisture to bite onto, then it seems to shrink and gives up the ghost.




i definitely agree with you on this…although I never had one give up…just took a while.

on the other hand, too wet plus too long of time colonizing from wedge drop can be bad too.

i don't want you to waste them, but I don't want you to fail either.  so if u used grains prepped like that before and they worked, then u should go for it.  just try not to simmer/boil them until "well done" next time.


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OfflineAgarStudent
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Re: Are these grains too wet? [Re: blindingleaf]
    #23600498 - 09/01/16 09:40 AM (7 years, 4 months ago)

Thanks for the feedback -

Do you think if they start to take off after a G-G transfer then they should be all good to go?
You would know pretty soon if they were bunk?, not like with too dry where you can wait a while!


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Invisibleblindingleaf
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Re: Are these grains too wet? [Re: AgarStudent]
    #23600610 - 09/01/16 10:12 AM (7 years, 4 months ago)

yea.  just shake it real good after u PC/inoculate to spread out any standing water. 

usually a PC cycle will make grains absorb some of that excess, but if they are already completely saturated, the water has nowhere to go.  usually it falls to the bottom of the jar during the cycle and causes those grains to burst, just as if you kept on boiling grains that were completely hydrated, just its under much higher PSI so bursting can happen easier.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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OfflineAgarStudent
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Re: Are these grains too wet? [Re: blindingleaf]
    #23602565 - 09/01/16 08:15 PM (7 years, 4 months ago)

Ah, okay.  That explains why I have seem more burst grains at the bottom!  Mycelium prefers not to colonize this?


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OfflinePsilosoulful

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Re: Are these grains too wet? [Re: AgarStudent]
    #23602702 - 09/01/16 08:46 PM (7 years, 4 months ago)

If it's all stuck together, then no, the mycelium will not be able to work its way through.


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OfflineAgarStudent
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Re: Are these grains too wet? [Re: Psilosoulful]
    #23603982 - 09/02/16 08:27 AM (7 years, 4 months ago)

A quick shake test and they came out of the PC with no residual water on the bottom, one or two popped grains, but they feel soft when you rotate the jar (if that makes sense?)

I am not sure what triggers the formation of scerlotia and it is dependent on the limitation of water as a trigger?  Definitely it appears that colonisation of the mycelium itself is limited when there is either insufficient water in the grains or the grains have exploded and are stuck together  (The other extreme).

Hopefully that gives a good margin for success in-between?  Thanks for the help.


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OfflineBaronVonBud
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Re: Are these grains too wet? [Re: AgarStudent]
    #23604219 - 09/02/16 10:26 AM (7 years, 4 months ago)

Does the toilet paper test work with grain ? It's perfect with WBS
Put a hand full on some TP count to 25 and dump it off. No wet spots = go time but that's WBS


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OfflineAgarStudent
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Re: Are these grains too wet? [Re: BaronVonBud]
    #23607251 - 09/03/16 08:50 AM (7 years, 4 months ago)

Thanks for the tip - RR has it in his video, but I didn't check it.
If it doesn't colonise then no problems starting fresh!
Sometimes I wonder it any water can ever escape through the SFD over time?


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Invisibleblindingleaf
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Re: Are these grains too wet? [Re: AgarStudent]
    #23607268 - 09/03/16 09:00 AM (7 years, 4 months ago)

grains can definitely dry out. usually room Rh, GE hole size, and filter material all affect howlongit might take


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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