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Offlinexwave3000
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Agar and cloning question
    #2359787 - 02/20/04 04:21 PM (14 years, 2 months ago)

What's the advantage of using malt extract and agar?
If i'm cloning a shroom, is there anything bad about transferring it directly to WBS jars instead of using agar?


Edited by xwave3000 (02/20/04 06:23 PM)


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OfflineAnnoA
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Re: Agar and cloning question [Re: xwave3000]
    #2375684 - 02/25/04 11:14 AM (14 years, 2 months ago)

>What's the advantage of using malt extract and agar?

For your purpose, you see better if the chunk you isolated is clean or not.

But yes, you can transfer it to grain directly.


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InvisibleLoki
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Re: Agar and cloning question [Re: xwave3000]
    #2375829 - 02/25/04 12:51 PM (14 years, 2 months ago)

You can clone straight to grain.
But using agar is a good way to ensure that your clone culture is
clean before transphering the clean colonised agar to grain.

Using agar, instead of just straight to grain, will increase
your success rate by quite a bit.

Most agar recipes are fine mea or pda are the most common.
If cloning dont forget to use mea + h202, as this will
also help your success rate a lot.


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