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OfflinePongdiddy
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Registered: 08/31/16
Posts: 3
Last seen: 7 years, 3 months
Liquid culture 3rd week, slow development? contam?
    #23596238 - 08/31/16 02:06 AM (7 years, 5 months ago)

Hi everyone

This is my first post.  I'm using liquid culture and following pf tek, and I'm a little concerned about the progress in this lc jar.  Spores were injected 3 weeks ago,  and I'm keeping the jar in a dark place at 23degC,  I only expose the jar to light to swill lc around,  approx every 24 hours.  Little balls of fluffy off white pseudo-mycelium have formed,  but there doesn't appear to be any whispy LC like in many photos I've seen here on shroomery. The fluffy balls separate from one another but do not disintegrate when swilled around and all gather together again when the water settles.  Is this normal/contam?  Any ideas or opinions greatly appreciated.


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Invisibleh0ldthedoor
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I'm a teapot User Gallery


Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Liquid culture 3rd week, slow development? contam? [Re: Pongdiddy]
    #23596513 - 08/31/16 06:39 AM (7 years, 5 months ago)

Here's a similar post, wherein the OP's LC created from spores is visibly contaminated. This may or may not be the case for you (there are a few dark spots in your jar but nothing compared to the linked post), but there is some good information in the responses.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish

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Invisibleblindingleaf
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Re: Liquid culture 3rd week, slow development? contam? [Re: h0ldthedoor]
    #23596520 - 08/31/16 06:42 AM (7 years, 5 months ago)

urs actually doesn't look bad but there is no way to tell if its clean just by looking at it.

the groups of growth are difficult to break up unless u have something in the bottom like shard of glass, marble, screw, etc.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflinePongdiddy
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Registered: 08/31/16
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Last seen: 7 years, 3 months
Re: Liquid culture 3rd week, slow development? contam? [Re: blindingleaf]
    #23597030 - 08/31/16 10:33 AM (7 years, 5 months ago)

Ok cool, thanks for the input guys :smile:

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OfflineRealmLAir
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Registered: 07/17/16
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Last seen: 2 years, 9 months
Re: Liquid culture 3rd week, slow development? contam? [Re: Pongdiddy]
    #23597333 - 08/31/16 12:41 PM (7 years, 5 months ago)

shake vigorously.

I like to shake it like a spraycan for a minute to break everything up. then I swirl it one way really fast for a few seconds then reverse the motion so it crashes into each other and comes to a halt. i also like to leave the jar tilted so that the mycelium sinks and forms in one corner persay. if you let it settle in the middle it forms a halo or donut.

i also found that my LC was fully developed at the 3 week mark. i made two jars, used one, and the other has been sitting ever since. it still looks the same. im sure more spores= more growth over time

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Invisibleh0ldthedoor
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Re: Liquid culture 3rd week, slow development? contam? [Re: RealmLAir]
    #23597646 - 08/31/16 02:07 PM (7 years, 5 months ago)

Quote:

blindingleaf said:
the groups of growth are difficult to break up unless u have something in the bottom like shard of glass, marble, screw, etc.




Quote:

RealmLAir said:
shake vigorously.

I like to shake it like a spraycan for a minute to break everything up. then I swirl it one way really fast for a few seconds then reverse the motion so it crashes into each other and comes to a halt.




A magnetic stirrer is perfect for getting things all mixed up. and it looks cool



The uploader used a 7.5v power supply and used a potentiometer which may not have been turned all the way up; if you need a more powerful vortex, use 12v instead.

:rockon:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish

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InvisiblemushboyMDiscord
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Re: Liquid culture 3rd week, slow development? contam? [Re: h0ldthedoor]
    #23597665 - 08/31/16 02:13 PM (7 years, 5 months ago)


if you look close you can see black specks in the LC.
i was a nooberino and shrugged it off. inoculated 12 brf jars
all turned into black mold death. never used lc after that..

I forgot the link or the poster but look up liquid inoculate tek(ill find the link soon i hope)
where you put a agar wedge in a jar of (sterilized) water and shake the shit
out of it. couldnt remember if the tek involved sucking it up in a syringe or just
dumping some into grain jars:rolleyes: but the tek had a video with a phish song..

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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
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Re: Liquid culture 3rd week, slow development? contam? [Re: mushboy]
    #23597908 - 08/31/16 03:23 PM (7 years, 5 months ago)

Is this it?

https://www.shroomery.org/forums/showflat.php/Number/22833314

It literally mentions "- shake the shit out of the water jar" and the video features a phish song


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish

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OfflinePongdiddy
Stranger
Registered: 08/31/16
Posts: 3
Last seen: 7 years, 3 months
Re: Liquid culture 3rd week, slow development? contam? [Re: h0ldthedoor]
    #23621764 - 09/07/16 11:56 AM (7 years, 5 months ago)

Thanks a lot, very helpful, I whilst the idea of LC sounds good, because of sheer amount of yield long term, i recon i'll leave it from now on, just too unreliable.

Cheers :smile:

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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Liquid culture 3rd week, slow development? contam? [Re: Pongdiddy]
    #23621799 - 09/07/16 12:11 PM (7 years, 5 months ago)

Liquid Culture is not inherently unreliable. However, Liquid Culture created from spores is inherently unpredictable. The Liquid Inoculate tek posted above isn't perfect, but it is likely to greatly increases your odds of success over using spores.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish

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InvisiblemushboyMDiscord
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Registered: 04/24/05
Posts: 32,502
Loc: where?
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Re: Liquid culture 3rd week, slow development? contam? [Re: h0ldthedoor]
    #23621815 - 09/07/16 12:17 PM (7 years, 5 months ago)

ah yes.. im late but thanks for that link.

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