Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleBunya
Male User Gallery

Registered: 06/04/16
Posts: 601
Mixing mycelium on agar
    #23596715 - 08/31/16 08:40 AM (7 years, 4 months ago)

Sorry if this has been asked before.
If i put two samples of mycelium together on agar from different phenotype spore prints but the same species would they blend together. And if it was a different species would they seperate from each other.
Just wondering if it could be used as an species identifying technique.
I hope my question is clear and understandable.
Thoughts.


--------------------
Bunya cones kill.


Extras: Filter Print Post Top
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Mixing mycelium on agar [Re: Bunya]
    #23597308 - 08/31/16 12:33 PM (7 years, 4 months ago)

No, and why not just look at the spores or get them DNA sequenced? This would be the hardest and least reliable way to ID a species.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
InvisibleinskiM
Cortinariologist
Male User Gallery


Registered: 02/28/06
Posts: 5,720
Trusted Cultivator
Re: Mixing mycelium on agar [Re: Bunya]
    #23599667 - 09/01/16 12:45 AM (7 years, 4 months ago)

Quote:

Bunya said:
If i put two samples of mycelium together on agar from different phenotype spore prints but the same species would they blend together. And if it was a different species would they seperate from each other.



Yes, if two samples of mycelium from different spore prints but the same species are grown on an agar plate it is very possible that anastomosis could occur between some of the many dikaryons that are present when they meet, if monokaryons are still present in the cultures mating may occur and dikaryons may form and be capable of producing fruit bodies.
If two colonies of mycelium from different species are grown on an agar plate mating will not occur and usually a distinct line can be seen where they meet.
It has been found that mycelium produces certain 'hormones' that attract compatible mycelium of the same species causing them to grow towards each other.

Quote:

Bunya said:
Just wondering if it could be used as an species identifying technique.



Mating compatibility testing has long been used by mycologists to determine conspecificity.


--------------------


Extras: Filter Print Post Top
InvisibleBunya
Male User Gallery

Registered: 06/04/16
Posts: 601
Re: Mixing mycelium on agar [Re: inski]
    #23599680 - 09/01/16 01:00 AM (7 years, 4 months ago)

Thanks inski.
Very precise answer like what i was after.
Thankyou.
Im thinking of playing with this.


--------------------
Bunya cones kill.


Extras: Filter Print Post Top
InvisibleinskiM
Cortinariologist
Male User Gallery


Registered: 02/28/06
Posts: 5,720
Trusted Cultivator
Re: Mixing mycelium on agar [Re: Bunya]
    #23599695 - 09/01/16 01:15 AM (7 years, 4 months ago)

No problem.

As 36 said, DNA sequencing is the main method for defining species now but it's not always entirely accurate.

Mating compatibility testing is really interesting but it requires some expertise and lots of agar plates, a method known as 'Dilution to Extinction' is very useful for diluting spore samples which is really helpful for isolating monokaryons.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Nobody sells bennaficial bacterial injections? Angel_Magic79 1,808 6 10/11/02 07:02 PM
by Suntzu
* Spawning Dung with Agar Anonymous 1,913 3 01/20/03 07:30 PM
by stonErollEr1
* Need suggestions about peroxidated agar CLuB99 1,499 4 08/11/01 04:18 PM
by Anno
* How to mix Peroxidated MEA Richard_D_James 2,129 7 03/27/02 02:43 PM
by ParticleMan
* agar, here's how you do it Una 1,673 7 10/30/02 01:13 AM
by zeronio
* Agar medium with honey AnnoA 3,566 2 05/24/02 06:44 PM
by Zen Peddler
* Re: Sorry for bothering you guys and gals AGAR QUESTION! Anonymous 871 1 01/21/00 03:27 PM
by Anonymous
* Honey mycelium... wikedanjel 2,924 8 07/30/02 09:05 AM
by CaliChronic

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
536 topic views. 1 members, 8 guests and 0 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.009 seconds on 14 queries.