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Bunya


Registered: 06/04/16
Posts: 601
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Mixing mycelium on agar
#23596715 - 08/31/16 08:40 AM (7 years, 4 months ago) |
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Sorry if this has been asked before. If i put two samples of mycelium together on agar from different phenotype spore prints but the same species would they blend together. And if it was a different species would they seperate from each other. Just wondering if it could be used as an species identifying technique. I hope my question is clear and understandable. Thoughts.
-------------------- Bunya cones kill.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Mixing mycelium on agar [Re: Bunya]
#23597308 - 08/31/16 12:33 PM (7 years, 4 months ago) |
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No, and why not just look at the spores or get them DNA sequenced? This would be the hardest and least reliable way to ID a species.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: Mixing mycelium on agar [Re: Bunya]
#23599667 - 09/01/16 12:45 AM (7 years, 4 months ago) |
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Quote:
Bunya said: If i put two samples of mycelium together on agar from different phenotype spore prints but the same species would they blend together. And if it was a different species would they seperate from each other.
Yes, if two samples of mycelium from different spore prints but the same species are grown on an agar plate it is very possible that anastomosis could occur between some of the many dikaryons that are present when they meet, if monokaryons are still present in the cultures mating may occur and dikaryons may form and be capable of producing fruit bodies. If two colonies of mycelium from different species are grown on an agar plate mating will not occur and usually a distinct line can be seen where they meet. It has been found that mycelium produces certain 'hormones' that attract compatible mycelium of the same species causing them to grow towards each other.
Quote:
Bunya said: Just wondering if it could be used as an species identifying technique.
Mating compatibility testing has long been used by mycologists to determine conspecificity.
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Mixing mycelium on agar [Re: inski]
#23599680 - 09/01/16 01:00 AM (7 years, 4 months ago) |
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Thanks inski. Very precise answer like what i was after. Thankyou. Im thinking of playing with this.
-------------------- Bunya cones kill.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: Mixing mycelium on agar [Re: Bunya]
#23599695 - 09/01/16 01:15 AM (7 years, 4 months ago) |
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No problem.
As 36 said, DNA sequencing is the main method for defining species now but it's not always entirely accurate.
Mating compatibility testing is really interesting but it requires some expertise and lots of agar plates, a method known as 'Dilution to Extinction' is very useful for diluting spore samples which is really helpful for isolating monokaryons.
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