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OfflineFalseMaria
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LI Syringe Still Good After 3 Months? (Refrigerated)
    #23596593 - 08/31/16 07:23 AM (7 years, 4 months ago)

I've got an LI Syringe and Jar that I've had in my culture fridge for several months. Any chance it's still viable?

I have colonized plates/tubes as well, so creating a new LI isn't a problem. I would just like to use what I already made if possible.


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Invisiblealoe
Registered: 10/27/15
Posts: 118
Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: FalseMaria]
    #23596640 - 08/31/16 07:58 AM (7 years, 4 months ago)

Won't know for sure unless you try. :P


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Offlinemicolatria
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: aloe]
    #23596677 - 08/31/16 08:23 AM (7 years, 4 months ago)

Quote:

aloe said:
Won't know for sure unless you try. :P




:thumbup:


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InvisiblebodhisattaMDiscordReddit
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: micolatria]
    #23596686 - 08/31/16 08:27 AM (7 years, 4 months ago)

Quote:

crack187 said:
Quote:

aloe said:
Won't know for sure unless you try. :P




:thumbup:



:thumbup:


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OfflineFalseMaria
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: bodhisatta]
    #23596930 - 08/31/16 09:56 AM (7 years, 4 months ago)

OK, let me rephrase the question; what are my odds that it's still viable?

I want to start this round successfully and as quickly as possible. If I have a 50/50 chance, I'll just make a new LI, and test the other stuff after that gets going.

I think I'll just make a new LI, and when I prep my spawn jars, I'll use a jar or two to test the old, and the rest the new.


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OfflineGreg
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: FalseMaria]
    #23596934 - 08/31/16 09:57 AM (7 years, 4 months ago)

Why not put a drop on agar and if it grows you're good to go?


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Invisiblewildernessjunkie
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: Greg]
    #23597430 - 08/31/16 01:06 PM (7 years, 4 months ago)

Quote:

FalseMaria said:
OK, let me rephrase the question; what are my odds that it's still viable?





No one can answer that. Theres a whole slew of variables in that question. The age or the original plate, the recipe used, Genetics, storage method and temps...the list could go on. Chances of it being viable are pretty good I would think, but:

Quote:

Greg said:
Why not put a drop on agar and if it grows you're good to go?




Thats the best answer you can probably get.


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OfflineMushierage
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: wildernessjunkie]
    #23597682 - 08/31/16 02:19 PM (7 years, 4 months ago)

Generally the answer is yes.  LIs can last a good long time if refrigerated.  But there's a lot of variables that can nullify viability. 

Put to agar.  If it looks good, shoot it into a jar if you want to risk it (ultra fast colonization) or just use the agar (not as fast but will be for sure clean)


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OfflineFalseMaria
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: Mushierage]
    #23598320 - 08/31/16 05:37 PM (7 years, 4 months ago)

OK, thanks. It comes from a plate from not very long ago either. MEA agar recipe. Should be good, so I'll knock a jar with it.


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Offlinewtfcrazymofo
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Re: LI Syringe Still Good After 3 Months? (Refrigerated) [Re: FalseMaria]
    #23598469 - 08/31/16 06:23 PM (7 years, 4 months ago)

hopefully.


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