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Rosesarefree
Stranger

Registered: 07/24/16
Posts: 26
Last seen: 3 years, 7 days
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Second grow(pics) looking ahead to agar and cloning
#23596559 - 08/31/16 07:10 AM (7 years, 4 months ago) |
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This is my second grow. Last time I did PF cakes in a SGFC with minimal success. Now I'm trying a chamberless grow using cakes spawned to coir. This technique has given me more personal time with the babies and given me a better understanding of their needs.
 Now I'm out of pink buffalo MS and wanting to experiment with agar. I've got everything ready just need to make it and select a mushroom to clone. And that brings me here, how do you guys select a clone? And do you have a favorite tek for the actual process? I'm using patsywhites easy agar tek but I'm still a bit unsure how to actually execute the cloning.
Thanks for any help!
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aloe
Registered: 10/27/15
Posts: 118
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Re: Second grow(pics) looking ahead to agar and cloning [Re: Rosesarefree]
#23596613 - 08/31/16 07:34 AM (7 years, 4 months ago) |
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Density, clusters, larger fruits, unique traits, etc.
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Rosesarefree
Stranger

Registered: 07/24/16
Posts: 26
Last seen: 3 years, 7 days
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Re: Second grow(pics) looking ahead to agar and cloning [Re: aloe]
#23596707 - 08/31/16 08:35 AM (7 years, 4 months ago) |
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Thank you! The cluster in the back right is my favorite right now
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guyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
Last seen: 5 years, 6 months
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Re: Second grow(pics) looking ahead to agar and cloning [Re: Rosesarefree]
#23596836 - 08/31/16 09:24 AM (7 years, 4 months ago) |
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for sure the back right cluster!
when you are ready to harvest, make sure your pasty plates are already made, cooled & ready to use.
harvest as usual, and take the cluster into the SAB where your pasty plate (or two or three)is already waiting.
Then, with a flamed scalpel, cut the base, where all the mushrooms seem to meet, and then take a small piece of the inside flesh on the tip of your scalpel, then open your pasty plate, quickly deposit the flesh, and close her up and label. Repeat a couple of times if you want to, but I usually only take one clone.
Thats it. leave it at room temp and you should see growth in a couple of days, and by day 4 or 5 it should be ready to transfer to a clean plate.
I always make at least one transfer from the clone plate and start from there, as the mushroom is not grown in sterile enviro, so you may have hidden cotams tagging along around the fruit.
Hope this helps
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