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OfflineGroovy Hobbit
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King oyster on straw contaminated - diagnosis
    #23596530 - 08/31/16 06:45 AM (7 years, 4 months ago)

I colonized straw in jars using stem butts. That worked nicely.

Then I tried spawning that to a small basket on 8/18, alternating layers of newly pasteurized straw - a plate on top, weighing down for gentle compression.

I put the basket in a plastic bag in which I poked maybe 20 holes, and put that in an empty SGFC in my basement, elevated.



I positioned cardboard loosely around the sides closest to light sources so that colonization would be semi-dark.

Now I've got a webby material showing which might be oyster mycelium (or mold), and a green contam:



What should I do next time to steer the ship away from these rocks?

Perhaps I should be monitoring the temperature during colonization, and making finer adjustments. I know it's generally been in the 70s (sometimes a little cooler) where it's colonizing...


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Invisiblefilthyknees
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Re: King oyster on straw contaminated - diagnosis [Re: Groovy Hobbit]
    #23596788 - 08/31/16 09:08 AM (7 years, 4 months ago)

I dunno, this forum is for psychedelics.


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OfflineGreg
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Re: King oyster on straw contaminated - diagnosis [Re: filthyknees]
    #23596889 - 08/31/16 09:44 AM (7 years, 4 months ago)

Guaranteed 100% fucked.

King oysters are finicky anyway and prefer wood in their substrate.

Why start with stem butts to straw jars? Sounds like a whole lot of places a contam could start.


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Invisiblewildernessjunkie
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Re: King oyster on straw contaminated - diagnosis [Re: Greg]
    #23597504 - 08/31/16 01:28 PM (7 years, 4 months ago)

I think Stamets created a lot of problems when touting that info about stem butts and slurries. He's made it sound overly simple, and leads to more failure than success. It CAN work, but its unreliable at best.

You're best off getting yourself a commercially prepared culture and following some more established techniques.


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OfflineShroomsh
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Re: King oyster on straw contaminated - diagnosis [Re: filthyknees]
    #23597588 - 08/31/16 01:49 PM (7 years, 4 months ago)

What was your method of pasteurization for the straw?

One of the biggest things that I would change based off of what you've said is you opened the bag too soon.  I put my spawn in 2-3 layers in pasteurized straw and then wrap that bag up TIGHT and throw it in the closet for about a month or so.  You shouldn't be poking holes in the bag that early. 

Both that white web and the green mold are nasty things and you should toss all of that right now.  Next time wait for full colonization before poking any holes. 

Quote:

filthyknees said:
I dunno, this forum is for psychedelics.


  And no, this forum is not exclusively for psychedelics.


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OfflineGreg
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Re: King oyster on straw contaminated - diagnosis [Re: Shroomsh] * 2
    #23597607 - 08/31/16 01:54 PM (7 years, 4 months ago)

Quote:

Shroomsh said:
Quote:

filthyknees said:
I dunno, this forum is for psychedelics.


  And no, this forum is not exclusively for psychedelics.





Mushroom Cultivation (115 viewing)
This forum is dedicated to all aspects of cultivating psychoactive mushrooms.

Proper place for gourmet mushrooms:
https://www.shroomery.org/forums/postlist.php/Board/13


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Invisiblefilthyknees
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Re: King oyster on straw contaminated - diagnosis [Re: Greg]
    #23597702 - 08/31/16 02:24 PM (7 years, 4 months ago)

Quote:

Greg said:
Quote:

Shroomsh said:
Quote:

filthyknees said:
I dunno, this forum is for psychedelics.


  And no, this forum is not exclusively for psychedelics.





Mushroom Cultivation (115 viewing)
This forum is dedicated to all aspects of cultivating psychoactive mushrooms.

Proper place for gourmet mushrooms:
https://www.shroomery.org/forums/postlist.php/Board/13



Lol


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineGroovy Hobbit
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Re: King oyster on straw contaminated - diagnosis [Re: Shroomsh]
    #23602773 - 09/01/16 09:08 PM (7 years, 4 months ago)

Quote:

Greg said:
Guaranteed 100% fucked.



Thought so. Thanks, man.

Quote:

Why start with stem butts to straw jars? Sounds like a whole lot of places a contam could start.



True. It wasn't well-conceived. I had the stem butts and realized I had a little straw ready, too - and just thought I'd see if they'd colonize it. They did.  wildernessjunkie's right: Stamets and many instructables-like sites make it seem easier than it is.
Quote:



Shroomsh said:
What was your method of pasteurization for the straw?



I rinsed it, then brought it to 180F-140F for 90 mins in water.
Quote:

One of the biggest things that I would change based off of what you've said is you opened the bag too soon.  I put my spawn in 2-3 layers in pasteurized straw and then wrap that bag up TIGHT and throw it in the closet for about a month or so.  You shouldn't be poking holes in the bag that early. 



Damn! Okay, I heard wrong. But thanks; I will definitely do that differently...

I've got syringes for a fresh start.


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OfflineGroovy Hobbit
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Re: King oyster on straw contaminated - diagnosis [Re: Groovy Hobbit]
    #23610779 - 09/04/16 09:48 AM (7 years, 4 months ago)

Question: Is it advisable to give the oyster baskets a last chance by moving them outside?

The reason I ask is that I've had green mold develop in a cubensis grain jar, carefully removed it (in a location completely separate from my other grows), and cased the rest of the colonized grain to manure outside - and the cubensis mycelium took off. The resulting patch continues to fruit nicely now.

Would those mold spores on the oyster straw pose a threat to my other outdoor grows a couple hundred feet away?

Thanks...


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Offlinemrmazdarx9
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Re: King oyster on straw contaminated - diagnosis [Re: Groovy Hobbit]
    #23610786 - 09/04/16 09:51 AM (7 years, 4 months ago)

Quote:

Groovy Hobbit said:
Question: Is it advisable to give the oyster baskets a last chance by moving them outside?

The reason I ask is that I've had green mold develop in a cubensis grain jar, carefully removed it (in a location completely separate from my other grows), and cased the rest of the colonized grain to manure outside - and the cubensis mycelium took off. The resulting patch continues to fruit nicely now.

Would those mold spores on the oyster straw pose a threat to my other outdoor grows a couple hundred feet away?

Thanks...




I'd imagine the contaminate spores would be floating around outside all the time anyway


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OfflineGroovy Hobbit
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Re: King oyster on straw contaminated - diagnosis [Re: mrmazdarx9]
    #23610811 - 09/04/16 10:00 AM (7 years, 4 months ago)

Exactly. Me, too. I can't imagine this little cluster would tilt the scales in favor of the green-mold armies...


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Invisiblemorty422
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Re: King oyster on straw contaminated - diagnosis [Re: Greg]
    #23610839 - 09/04/16 10:09 AM (7 years, 4 months ago)

Quote:

Greg said:
Quote:

Shroomsh said:
Quote:

filthyknees said:
I dunno, this forum is for psychedelics.


  And no, this forum is not exclusively for psychedelics.





Mushroom Cultivation (115 viewing)
This forum is dedicated to all aspects of cultivating psychoactive mushrooms.

Proper place for gourmet mushrooms:
https://www.shroomery.org/forums/postlist.php/Board/13




:burn:


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