Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags   Bridgetown Botanicals Bridgetown Botanicals

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblelittlespider
spider
Female User Gallery

Registered: 05/21/14
Posts: 496
Loc: UK
Looking at spores with microscope..
    #23596396 - 08/31/16 04:55 AM (7 years, 4 months ago)

I got a spore print from Protostropharia on a slide  and viewed it under 1200x. Compared to lib print. Expected to see a significant difference. Simialr colour and shape...


Thinking the value of spot checking and learning to ID using microscope is that galerina looks more orange and death cap is more chubby and round (only viewed spore pics online so far)?

I'm guessing to see really distinct features of a spore like a lib I would need a more powerful magnification?


--------------------
remember what the dormouse said


Extras: Filter Print Post Top
OfflineJoie
 User Gallery

Registered: 10/17/09
Posts: 7,301
Loc: UK Flag
Last seen: 1 year, 3 months
Trusted Identifier
Re: Looking at spores with microscope.. [Re: littlespider]
    #23596425 - 08/31/16 05:23 AM (7 years, 4 months ago)

Is that a proper microscope? 1200x is more than you get with a regular set of objectives and the lens would have to be a good immersion lens to get a clear image. Notes on morphology and micromorphology are all useful in keying a species, but as with macromorphology you need to know what to look for. I always thought I was rubbish at this but recently it became a lot easier on someone else's kit, I could see clearly and measure everything (so I need new kit; the illumination has gone now anyway). So long as you have good optics and a graticule you should be able to note the distinguishing features and arrive at an ID more often than not. Some spores are very different and you can use the appearance without needing to measure. Others are pretty similar ovals in which case you do want sufficiently good optics to distinguish ornaments and germ pores and such.


--------------------


Extras: Filter Print Post Top
Invisiblelittlespider
spider
Female User Gallery

Registered: 05/21/14
Posts: 496
Loc: UK
Re: Looking at spores with microscope.. [Re: Joie]
    #23599869 - 09/01/16 03:10 AM (7 years, 4 months ago)

Ah I don't think it's an emersion lens. The lens dosnt focus close enough to the glass to rest it in a liquid.
Probabaly colour and shape is as good as I will spot with this scope. It was super cheap.
Thanks for your reply


--------------------
remember what the dormouse said


Extras: Filter Print Post Top
OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,276
Last seen: 4 hours, 33 minutes
Trusted Identifier
Re: Looking at spores with microscope.. [Re: littlespider]
    #23600293 - 09/01/16 08:31 AM (7 years, 4 months ago)

You should probably get a real scope with an oil immersion objective, something like an Amscope B-120ms.

For this species you'll need to look at the germ pore - There is Protostropharia semiglobata and S. dorsipora.  Does it have a small, off center germ pore or a larger germ pore in the center of the end of the spore? 

See https://books.google.com/books?id=RLXrCgAAQBAJ&lpg=PA110&dq=protostropharia%20dorsipora&pg=PA111#v=onepage&q=protostropharia%20dorsipora&f=false


Extras: Filter Print Post Top
Invisiblelittlespider
spider
Female User Gallery

Registered: 05/21/14
Posts: 496
Loc: UK
Re: Looking at spores with microscope.. [Re: Alan Rockefeller]
    #23603661 - 09/02/16 03:58 AM (7 years, 4 months ago)

Damn my impetuosity ordering a cheap one!

Yeah I don't think I can see that detail :/


--------------------
remember what the dormouse said


Extras: Filter Print Post Top
OfflineMrcloudy
Stranger than you.
Male User Gallery

Registered: 10/01/13
Posts: 2,889
Loc: Northeast US Flag
Last seen: 3 months, 20 days
Trusted Identifier
Re: Looking at spores with microscope.. [Re: littlespider]
    #23603807 - 09/02/16 06:26 AM (7 years, 4 months ago)

Measurement of spores is also an important detail a stage micrometer and a program like Piximetre is incredibly helpful.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


Extras: Filter Print Post Top
Invisiblelittlespider
spider
Female User Gallery

Registered: 05/21/14
Posts: 496
Loc: UK
Re: Looking at spores with microscope.. [Re: Mrcloudy]
    #23604465 - 09/02/16 11:59 AM (7 years, 4 months ago)

Thanks I shall save my pennies


--------------------
remember what the dormouse said


Extras: Filter Print Post Top
InvisibleKenInVic
Hey Bulldog
Male User Gallery


Registered: 03/01/16
Posts: 1,452
Loc: 3rd Stone from the Sun Flag
Re: Looking at spores with microscope.. [Re: Alan Rockefeller]
    #23643240 - 09/13/16 10:19 PM (7 years, 4 months ago)

Quote:

Alan Rockefeller said:
You should probably get a real scope with an oil immersion objective, something like an Amscope B-120ms.




I grabbed this very one based on our PM conversation.  It arrived today and I'm quite happy with it.  I picked up both the 10x and 20x oculars with it and the 3 MP camera for taking pics.

I'm not as happy with the camera as I am with just looking through the regular optics at this point.  Might need to use my tower and its monitor instead of doing this on the laptop.  Have a bit of a learning curve coming, methinks.

Anyway, thanks for your help on that.


--------------------
***My SGFC***  ***ID Mushrooms Here***
Pondering the question, "Are we all here, because we're not all there?"
       

"Because something is happening here, but you don't know what it is, do you, Mr. Jones."  Ballad of a Thin Man by Mrs. Zimmerman's little boy, Bobby.


Extras: Filter Print Post Top
OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,276
Last seen: 4 hours, 33 minutes
Trusted Identifier
Re: Looking at spores with microscope.. [Re: KenInVic]
    #23654513 - 09/17/16 11:43 PM (7 years, 4 months ago)

Try using a cell phone to take pics.  It can be hard to hold it in the right spot, but the image quality is usually a lot better than those digital eyepieces.


Extras: Filter Print Post Top
InvisibleKenInVic
Hey Bulldog
Male User Gallery


Registered: 03/01/16
Posts: 1,452
Loc: 3rd Stone from the Sun Flag
Re: Looking at spores with microscope.. [Re: Alan Rockefeller]
    #23654558 - 09/18/16 12:07 AM (7 years, 4 months ago)

My cell phone is a dumb phone.  Flip with really stupid camera and no OS.  Can't find an adapter for my Pentax and the price difference with the camera wasn't bad. It's 3MP.  Here's my first attempt at spores from Leucoagaricus barssii.  400x magnification.



--------------------
***My SGFC***  ***ID Mushrooms Here***
Pondering the question, "Are we all here, because we're not all there?"
       

"Because something is happening here, but you don't know what it is, do you, Mr. Jones."  Ballad of a Thin Man by Mrs. Zimmerman's little boy, Bobby.


Edited by KenInVic (09/18/16 12:08 AM)


Extras: Filter Print Post Top
OfflineToxicManM
Bite me, it's fun!
 User Gallery

Registered: 06/28/02
Posts: 6,722
Loc: Aurora, Colorado
Last seen: 5 hours, 54 minutes
Trusted Identifier
Re: Looking at spores with microscope.. [Re: KenInVic]
    #23655007 - 09/18/16 08:43 AM (7 years, 4 months ago)

That's actually a pretty good photo. Keep it up!


--------------------
Happy mushrooming!


Extras: Filter Print Post Top
Invisiblelittlespider
spider
Female User Gallery

Registered: 05/21/14
Posts: 496
Loc: UK
Re: Looking at spores with microscope.. [Re: ToxicMan]
    #23660566 - 09/20/16 02:36 AM (7 years, 4 months ago)



--------------------
remember what the dormouse said


Extras: Filter Print Post Top
OfflineJoie
 User Gallery

Registered: 10/17/09
Posts: 7,301
Loc: UK Flag
Last seen: 1 year, 3 months
Trusted Identifier
Re: Looking at spores with microscope.. [Re: littlespider]
    #23660588 - 09/20/16 03:08 AM (7 years, 4 months ago)

The Brunel SP22 is very popular and inexpensive, but it is monocular whereas it's much easier to fix your eyes into two cups for long periods. Prices are a lot higher for SP40 binoculars, of a higher grade. Those are the only models in their standard range that should interest you (digitals and accessories aside) however they have fingers in a few pies and it is perhaps worth giving them a call. If buying anything 2nd-hand ensure the optics are fully refurbished. http://www.brunelmicroscopes.co.uk/index.html I have only bought lenses from them but pretty much everyone goes straight there.

EDIT: There is currently an SP40 on this page that they say is good and you could ask about: http://www.usedmicroscopes.co.uk/budget.html


--------------------


Edited by Joie (09/20/16 03:12 AM)


Extras: Filter Print Post Top
InvisibleKenInVic
Hey Bulldog
Male User Gallery


Registered: 03/01/16
Posts: 1,452
Loc: 3rd Stone from the Sun Flag
Re: Looking at spores with microscope.. [Re: littlespider]
    #23661044 - 09/20/16 09:11 AM (7 years, 4 months ago)

Quote:

littlespider said:
That is a good photo.

One like this?
https://www.amazon.co.uk/gp/aw/d/B00X6IGN5O/ref=mp_s_a_1_1?ie=UTF8&qid=1474360522&sr=8-1&pi=SY200_QL40&keywords=amscope+b120c&dpPl=1&dpID=51YAuMpeb0L&ref=plSrch


Expensive eek




You probably don't need the microscope book or the prepared slides.  The model I picked up is the B120B-3M.  My main concern was a decent USB camera with it.  If you don't need the camera, the B120B could be enough.

Try Amscope's site.  I did better there than I could off Amazon.  They come out of California.  I'm in Canada and got nailed with duties, which I imagine you would as well.


--------------------
***My SGFC***  ***ID Mushrooms Here***
Pondering the question, "Are we all here, because we're not all there?"
       

"Because something is happening here, but you don't know what it is, do you, Mr. Jones."  Ballad of a Thin Man by Mrs. Zimmerman's little boy, Bobby.


Edited by KenInVic (09/20/16 09:15 AM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags   Bridgetown Botanicals Bridgetown Botanicals


Similar ThreadsPosterViewsRepliesLast post
* Question about Microscopes and IDing
( 1 2 all )
cheesenoonions 5,287 23 11/17/02 07:47 PM
by ToxicMan
* Re: spore prints.... Psylosymon 1,596 1 01/05/00 08:38 PM
by Psylosymon
* Prints and Microscope acenXA 2,341 13 09/04/02 03:37 AM
by ToxicMan
* Positive ID with a Microscope sublimished 2,172 5 03/23/04 06:54 PM
by Anonymous
* Amscope Question Chrontaunomo 464 3 10/27/12 10:18 AM
by suchen
* Another identify pls benzene 1,184 8 06/27/04 09:43 AM
by ToxicMan
* Question about measurement settings on ToupView Amscope camera software RiverDweller1 2,517 14 11/02/13 12:02 PM
by Byrain
* Identification of mushrooms through spores only? ragadinks 1,981 12 08/14/04 03:05 PM
by ragadinks

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: ToxicMan, inski, Alan Rockefeller, Duggstar, TimmiT, Anglerfish, Tmethyl, Lucis, Doc9151, Land Trout
748 topic views. 1 members, 26 guests and 5 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 14 queries.