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RealmLAir
Stranger
Registered: 07/17/16
Posts: 94
Last seen: 2 years, 8 months
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Try again,again,again..
#23592122 - 08/30/16 12:09 AM (7 years, 4 months ago) |
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1st attempt: ordered spore syringes off vendor. attempted 6 BRF jars with no PC. used a whole syringe in that run....saw NO progress whatsoever. eventually saw green in one jar. they all looked dried out so i threw two out and literally forgot the rest until this very moment writing these words...just checked on them and they are ALL Contamed to hell and back. cobweb, and black and green molds. I took a quick peek and closed the door. Ill be throwing them out ASAP 
2nd attempt: gotta PC. made 2 new jars using LC i made from the spore syringe. Got to place one in the FC (i assumed it was 100% colonized, but it didnt pin after 5 days so idk) the other one was still in progress.
2.5 attempt?: inoculated 4 more jars and was waiting for their birth date.
The other day i was checking on some jars and fanning my sgfc in the closet area i have. and the worse thing possible happened...my shelf came crashing down into the open FC! 8 pasty plates, 11 jars (5 at 60+ % and 6 fresh out the PC not inoculated yet) and verm was everywhere. the shelf almost landed,on my cake that was in the FC. luckily it was just one cake in there. on its 5th day no pinning, so it wasnt a big loss.
However, instead of tossing everything out, i used the Lazy mofo bag tek to try to salvage the cakes. I opened and crumbled three brf cakes that were at about 60 percent and also crumbled the one cake that was at 100% in the FC already. I made TWO bags, each one had 3 fresh brf cakes mixed with 2 partially colonized cakes. i rinsed off and got rid of the uncolonized portion so i figure the ratio is more like 3 to 1 in each bag.
after 3 days i am definitely seeing progress in one of the bags. i imagine it'll be done in 4 more. the other bag had bigger chunks due to the Fully colonized cake being in there, so i crumbled it more yesterday. hoping it shows some progress in the next few days.
I know the lazy mofo tek said it took a total of 3/4 days for full colonization...but keep in mind i used partially colonized cakes instead.
TL/DR?:: Jars fell, all projects were compromised, so i utilized LAzy mofo bag tek and its working. No contams thus far.
Edited by RealmLAir (08/30/16 01:14 AM)
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Try again,again,again.. [Re: RealmLAir]
#23592202 - 08/30/16 01:09 AM (7 years, 4 months ago) |
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keep reading.. Learn agar.. Be rewarded.. 
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RealmLAir
Stranger
Registered: 07/17/16
Posts: 94
Last seen: 2 years, 8 months
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Re: Try again,again,again.. [Re: liloldme]
#23592211 - 08/30/16 01:16 AM (7 years, 4 months ago) |
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I am getting agar. I have 5 plates all with good healthy mycelium covering it...but I understand there's not much I can do with agar and pf tek
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Try again,again,again.. [Re: RealmLAir]
#23592239 - 08/30/16 01:32 AM (7 years, 4 months ago) |
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Use your agar to clean up your mycelium before you use it in your liquid cultures by making a couple healthy transfers. You can use a chunk of it to colonize jars(G2G) or to start a LC. You can use this on pf jars but you should really try grain. Search for foomans WBS tutorial
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TheLongComma
Sky Raisin


Registered: 08/07/16
Posts: 50
Last seen: 2 years, 9 months
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Re: Try again,again,again.. [Re: liloldme]
#23592321 - 08/30/16 02:58 AM (7 years, 4 months ago) |
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Assuming you're following a PF tek:
Use half pint jars. Pint jars tend to stall. (You can use them, but just put verm in the bottom as pasty points out)
Also - do your lids have a way for GE to occur?
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RealmLAir
Stranger
Registered: 07/17/16
Posts: 94
Last seen: 2 years, 8 months
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Quote:
TheLongComma said: Assuming you're following a PF tek:
Use half pint jars. Pint jars tend to stall. (You can use them, but just put verm in the bottom as pasty points out)
Also - do your lids have a way for GE to occur?
the pictures in my Op were my first attempt were i didn't have the right jars or PC.
I do use 1/2 pints. I'm following the tek to a T, so I have no problem with stalled growth or GE.
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