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Offlinewtfcrazymofo
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Registered: 07/26/15
Posts: 1,201
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How many generations can you clone?
    #23589237 - 08/29/16 07:42 AM (7 years, 4 months ago)

Ms to fruit, then clone that fruit. Then grow that out, clone another fruit.  Then grow out again and clone that fruit.  Then grow that out and clone that fruit.

Can you just keep cloning fruits generation after generation?

I've read somewhere that the PE strain was created like this by picking out the fattest ones each time and cloning it.


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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InvisiblePsychedelic Pupil
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Registered: 09/27/12
Posts: 744
Loc: The bright side of life
Re: How many generations can you clone? *DELETED* [Re: wtfcrazymofo]
    #23589249 - 08/29/16 07:52 AM (7 years, 4 months ago)

Post deleted by Psychedelic Pupil

Reason for deletion: I was wrong. Thanks for the clarification Pasty



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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: How many generations can you clone? [Re: Psychedelic Pupil]
    #23589275 - 08/29/16 08:04 AM (7 years, 4 months ago)

Everytime you grow out a fruit you are dramatically increasing the number of cell divisions on that culture. As the cells divide the telomeres get shorter. If the telomeres get too short you end up with senscence. Eventually it reaches a point where it contams easy, has no vigor, and finally dies.

With proper care a clone could last decades. Cloning clones will probably cause the culture to die in a year or less.


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Offlinewtfcrazymofo
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Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 35 minutes
Re: How many generations can you clone? [Re: Pastywhyte]
    #23589344 - 08/29/16 08:35 AM (7 years, 4 months ago)

If you clone a bunch of times then take a print, would that clone out a bunch more times?

If so I guess it would most likely become more of a domesticated weaker mushroom.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
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Re: How many generations can you clone? [Re: wtfcrazymofo]
    #23589433 - 08/29/16 09:23 AM (7 years, 4 months ago)

Going back to spores is essentially a reset. Senscence is not an issue there.


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