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Invisibleeasyskunkin

Registered: 12/23/03
Posts: 565
cubensis affected by gentamycin sulfate ?
    #2358797 - 02/20/04 11:52 AM (12 years, 10 months ago)

hello, I read in TMC about the use of gentamycin sulfate in agar media to prevent development of bacillus, but is stated that some species of mushrooms are adversly affected by this antibiotic : so can someone tell me if the cubensis are affected in any way by gentamycin sulfate, and if not what is the dosage for 1 liter of Potatoe Dextrose Agar and under what form should I use it ? thanks for any help Pedro


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L'intérêt à croire une chose n'est pas une preuve de l'existence de cette chose.
Voltaire ; Remarques sur les pensées de Pascal (1728)


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: cubensis affected by gentamycin sulfate ? [Re: easyskunkin]
    #2358877 - 02/20/04 12:22 PM (12 years, 10 months ago)

Work on sterilization teks before using any sort of antibiotic . No it will not harm P.cubes . But why add this if you can stop contams naturally ?
Page 20 of TMC ..., 0 .10 grams of 60-80% pure gentamycin sulfate to each litre of media prior to sterilization.
Good Luck
Trip n D


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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Invisibleeasyskunkin

Registered: 12/23/03
Posts: 565
Re: cubensis affected by gentamycin sulfate ? [Re: tripndicular]
    #2359606 - 02/20/04 03:44 PM (12 years, 10 months ago)

thank you I hadn't seen the mention in TMC, I don't have a problem for now apparently since this translucent gelly I have seems to be normal according to the answers I got on the website. just checking about the technique thanks a lot Pedro


--------------------
L'intérêt à croire une chose n'est pas une preuve de l'existence de cette chose.
Voltaire ; Remarques sur les pensées de Pascal (1728)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: cubensis affected by gentamycin sulfate ? [Re: easyskunkin]
    #2359644 - 02/20/04 03:50 PM (12 years, 10 months ago)

Got a pic of this "jelly " you mentioned , and if in grain gent will not help there , that sounds like inproper prep of grain , or a contam that may have got in during innoc .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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