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OfflineAgarStudent
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Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
ATL#7 Progress Questions
    #23585906 - 08/28/16 06:14 AM (7 years, 5 months ago)

Jar #1
Inoculation date: 25th July (just over a month)
Inoculation style:  Wedge from first agar grow, mixed sectors (prior to isolating sectors)
Did I shake the jar? I can't remember?



Jar #2
Inoculation date: 6th August (Just under a month)
Inoculation style:  Wedge from sector isolation
Did I shake the jar?  obviously



My question is why would jar1 fare so well and jar2 not well at all?
--> Could it be a problem with isolating a crappy sector (or do most sectors come out similar?)
--> Could it be a problem with grain hydration (supposing I didn't boil the prep grain)
--> Could it be a problem with shaking the grain?

What do you recommend to do with the second jar? Let it go or scrap it?

Thanks for any tips guys :smile:


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InvisibleCosmoKramer
The Assman

Registered: 06/22/16
Posts: 555
Re: ATL#7 Progress Questions [Re: AgarStudent]
    #23588490 - 08/28/16 09:25 PM (7 years, 5 months ago)

Looks like jar 2 grains are dry. Did you prep and sterilize the at the same time or separate?


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OfflineAgarStudent
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Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
Re: ATL#7 Progress Questions [Re: CosmoKramer]
    #23589212 - 08/29/16 07:20 AM (7 years, 4 months ago)

Ah thanks for the feedback too CosmoKramer.
Truth is I think I forgot to boil, which did not fully hydrate the grains. 
Now I bring to the full boil for ten minutes, as per RR grain-prep video.

I dropped in a wedge, well after it had cooled, but it just wasn't digging the grains...


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