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teedub
Stranger
Registered: 07/23/16
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Last seen: 7 years, 4 months
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Rye Jars Stalled?
#23584046 - 08/27/16 03:52 PM (7 years, 5 months ago) |
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Hello all, long time reader...first time poster.
I inoculated 4 rye jars on Aug 8th using spore syringes. I seen first signs of mycellium on Aug 18th in two jars. I threw on of those jars out on the 22nd for having green mold growing.
Today is the 27th and the only jar that has signs only have one spot the size of a quarter and another spot smaller than a dime. The remaining 2 are showing no signs.
The temp is 73 average (house stays at around 72 at night and 74 during the day) and they get about 10 hours of sunlight as well. The jar lids have injector ports and gas exchange material over another hole.
I would think that after 3 weeks, there would be more growth...even from spore syringes.
So does anyone have any insight on why they are stalling and how to jump start them back up? Increasing heat to 78 or so? Usually when I go BRF, they are pretty much ready to dunk and roll by now.
Thanks in advance.
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vikingleif
Lazy Stoner



Registered: 08/11/16
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Re: Rye Jars Stalled? [Re: teedub]
#23584057 - 08/27/16 03:56 PM (7 years, 5 months ago) |
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you might have bacteria and thats what causing the stalling.
post a pic and let the experienced growers have a look at it  always better with a pic
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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teedub
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Inocuole
Scalpel of Evil's Bane



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Re: Rye Jars Stalled? [Re: teedub] 1
#23584107 - 08/27/16 04:09 PM (7 years, 5 months ago) |
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Invisible, divisible, wasting your material, Slow growth, no growth, yep that's bacterial.
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teedub
Stranger
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Re: Rye Jars Stalled? [Re: teedub]
#23584112 - 08/27/16 04:10 PM (7 years, 5 months ago) |
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I had bought 4 jars that were pre-made and ready for inoculation form a vendor here and after 3 weeks and this is the only growth in the only jar.
I have since then prepared 8 other rye jars on my own (4 days ago) and I am waiting to see their progress.
But those are the only signs of life.
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SuperFly
Still in the Space Race



Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
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Re: Rye Jars Stalled? [Re: teedub]
#23584118 - 08/27/16 04:11 PM (7 years, 5 months ago) |
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Is the syringe from a reliable sorce? Don't mention where it is from too please. This can also be a problem. If you do plan on doing more and more bulk look into agar. A second guess problem such as this would be a thing of the past.
Edit. just read it was reliable, for sure look into agar then.
Edited by SuperFly (08/27/16 04:12 PM)
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Inocuole
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Re: Rye Jars Stalled? [Re: SuperFly]
#23584136 - 08/27/16 04:14 PM (7 years, 5 months ago) |
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The syringe being from a reliable source doesn't mean there won't be bacteria in it. And even if it were a 100% clean syringe, grain can be bacterial on its own from improper sterilization, or a particularly high endospore load. Or from the injector port. Or from some manner of procedure with the syringe, like wiping the needle after flaming. Tons of vectors.
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teedub
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Re: Rye Jars Stalled? [Re: SuperFly]
#23584151 - 08/27/16 04:17 PM (7 years, 5 months ago) |
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The syringes also came from a trusted supplier that I have used many times before.
That is why I am very confused as to why 2 jars completely flunked, one became contaminated, and one is stalled. I had alcohol sterilized everything, flame sterilized the syringe between jars, and put 1cc on one side of the jar and 1cc on the other side.
My plan was to buy new spore syringes, prepared jars from a trusted vendor here, inoculate those jars to get a jumpstart. While the prepared vendor jars are germinating, I was then going to prepare my own rye jars for G2G for my monotubs.
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Inocuole
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Re: Rye Jars Stalled? [Re: teedub]
#23584157 - 08/27/16 04:17 PM (7 years, 5 months ago) |
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Alcohol doesn't sterilize anything. That could be an issue depending on what you mean. If you buy pre-sterilized grain you're just going to keep having bacteria issues. Grain is never truly sterile unless you PC it for like a day or 2 straight, at which point it's mush.
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SuperFly
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Re: Rye Jars Stalled? [Re: Inocuole]
#23584165 - 08/27/16 04:18 PM (7 years, 5 months ago) |
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Just trying to help and narrow down the problem. While a reliable spore vendor can still have bacteria contamination, it is less of a chance Quote:
Inocuole said: The syringe being from a reliable source doesn't mean there won't be bacteria in it. And even if it were a 100% clean syringe, grain can be bacterial on its own from improper sterilization, or a particularly high endospore load. Or from the injector port. Or from some manner of procedure with the syringe, like wiping the needle after flaming. Tons of vectors.
Just trying to help and narrow down the problem. While a reliable spore vendor can still have bacteria contamination or mold, the % of this happening is a lot less.
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teedub
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Re: Rye Jars Stalled? [Re: Inocuole]
#23584169 - 08/27/16 04:19 PM (7 years, 5 months ago) |
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I alcohol swabbed the injection port and syringes prior to flame and inoculation.
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Inocuole
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Re: Rye Jars Stalled? [Re: SuperFly]
#23584176 - 08/27/16 04:20 PM (7 years, 5 months ago) |
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For the purpose of shooting spores onto grain directly, there is no such thing as a reliable vendor. Most vendor syringes work fine with PF cakes, and that's what syringes are made for basically. When you start fucking with grains it's time to actually get a clean culture to inoculate with.
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SuperFly
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Re: Rye Jars Stalled? [Re: SuperFly]
#23584197 - 08/27/16 04:24 PM (7 years, 5 months ago) |
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Quote:
SuperFly said: for sure look into agar then.
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teedub
Stranger
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Re: Rye Jars Stalled? [Re: Inocuole]
#23584205 - 08/27/16 04:27 PM (7 years, 5 months ago) |
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Quote:
Inocuole said: For the purpose of shooting spores onto grain directly, there is no such thing as a reliable vendor. Most vendor syringes work fine with PF cakes, and that's what syringes are made for basically. When you start fucking with grains it's time to actually get a clean culture to inoculate with.
Well I am aware of that but it is kind of hard to get a clean culture started from nothing when I have no resources at hand.
I guess I could have BRFd, fruited, then started agar but that would take a hell of a lot longer than doing rye. Hence why I assumed that buying a syringe from a vendor and prepared jars ready for inoculation would be faster. Apparently not.
I guess the many people here on this forum that have done spore syringes to rye jars are just miracles. I would think that my chances would have been on the high percentile considering. Hindsight says not.
But by all means....if you would like to send me some clean cultures, I would appreciate that.
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bodhisatta 
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Re: Rye Jars Stalled? [Re: teedub]
#23584228 - 08/27/16 04:30 PM (7 years, 5 months ago) |
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pre made jars + spore syringe = even worse odds though
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teedub
Stranger
Registered: 07/23/16
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Last seen: 7 years, 4 months
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Quote:
Trusted Cultivator said: pre made jars spore syringe = even worse odds though
With their high ratings here...I assumed incorrectly that it would have been faster and more reliable until I could get my own set up started.
Since then, I have inoculated 8 other jars that I prepared myself with 4 other types of mushrooms so I will soon see if it was just that one syringe or the vendors jars.
So is it everyone's consensus according to the information given and the pictures that it is a bacterial issue from either the jars or the syringe?
No one has any ideas on how to get the jar with the current mycelium to speed up or does the rhizo look too funky and just go ahead and abort?
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Inocuole
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Re: Rye Jars Stalled? [Re: teedub]
#23584270 - 08/27/16 04:41 PM (7 years, 5 months ago) |
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If you asked how the actual users felt you'd see that nobody would give them high ratings. They don't have high ratings here. They have high ratings on their site where they can control the ratings.
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Tuhdoww
Sub Slapper


Registered: 08/23/16
Posts: 300
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Re: Rye Jars Stalled? [Re: Inocuole]
#23584279 - 08/27/16 04:44 PM (7 years, 5 months ago) |
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Quote:
Inocuole said: Invisible, divisible, wasting your material, Slow growth, no growth, yep that's bacterial.
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teedub
Stranger
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Re: Rye Jars Stalled? [Re: Inocuole]
#23584301 - 08/27/16 04:50 PM (7 years, 5 months ago) |
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That is very true. I have full faith in the syringes though because I have been using the same place for about 10 years here and there when I get a grow going.
This was my first try with the vendor who does the jars but with the reviews he received here from so many people, I had no reason to question his quality.
So many things can go wrong on a grow, which is why I went with these vendors...to hopefully minimize the chances of myself fucking something up.
As I have stated, I will see how my personally prepared jars do and compare. I may just scrap the entire project and start from scratch and try to do syringe to agar or BRF but then I would be even more behind on my grow schedule by months. But that is the way things happen sometimes.
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