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Offlineglobejs
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Micropore tape for PCing master slant tubes?
    #23582934 - 08/27/16 09:56 AM (7 years, 5 months ago)

So I have a quick question about the pcing process I'm going to do as I have some test tubes I've never used before.

I bought a few test tubes with a rack from shroom supply and they came with these loose fitting plastic caps that create no seal.

As far as sterilizing them goes, I thought I should seal them up with micropore tape before they go in to create  a  filter  for the cooling process and just to keep them sterile. Is this a good idea?

RR's video shows him with screw caps that he puts down tight so there's no need for a filtered tape.

Also should I just pc them in the rack or place them in wide mouth pint jars with water in them  to hold them up in the pc?

I'm going to also pc a wine bottle of agar for some disposable plates at the same time.


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Offlineglobejs
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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23582941 - 08/27/16 10:00 AM (7 years, 5 months ago)



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InvisiblePastywhyteMDiscord
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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583002 - 08/27/16 10:21 AM (7 years, 5 months ago)

You don't want them sealed when you PC them. That will end up with a bunch of cracked tubs. RR does the caps up loose but not tight when he sterilizes then.


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Offlineglobejs
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Re: Micropore tape for PCing master slant tubes? [Re: Pastywhyte]
    #23583058 - 08/27/16 10:37 AM (7 years, 5 months ago)

Actually that's not true. Watch the chapter on master slants on youtube. At exactly 3.5 minutes in he says, "now because of the fact that  we screwed the lids down tight we don't want to do anything to speed up the cool down process or you'll end up exploding your test tubes."


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Offlineglobejs
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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583069 - 08/27/16 10:39 AM (7 years, 5 months ago)

And if you look at the caps I have and my question, that's not really my problem as there's no way for me to seal them tight. I was wondering about the use of micropore tape as a filter as whether to add it before the pcing


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OfflinePsilosopherr
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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583074 - 08/27/16 10:42 AM (7 years, 5 months ago)

thanks for the heads up about that vendor...I'd be pissed if I were you


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Invisibledankington
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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583076 - 08/27/16 10:43 AM (7 years, 5 months ago)

Quote:

After you fill each one, push the cap on but only across the first snap. It should jiggle loosely but not come off easily- this will allow GE during sterilization.




https://www.shroomery.org/forums/showflat.php/Number/18182209#18182209

Are those the same tubes?

You should use parafilm after you've inoculated them.


Edited by dankington (08/27/16 10:44 AM)


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OfflinePsilosopherr
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Re: Micropore tape for PCing master slant tubes? [Re: dankington]
    #23583091 - 08/27/16 10:47 AM (7 years, 5 months ago)

Like even if he does PC and inoculate properly he'd have to seal after colonization to prevent moisture loss right? Duct tape maybe? oof


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Re: Micropore tape for PCing master slant tubes? [Re: Psilosopherr]
    #23583099 - 08/27/16 10:49 AM (7 years, 5 months ago)

well, I don't think so. My masters aren't shut completely or they'd die.

I leave them a touch unscrewed and cover with parafilm.


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Offlineglobejs
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Re: Micropore tape for PCing master slant tubes? [Re: dankington]
    #23583146 - 08/27/16 10:59 AM (7 years, 5 months ago)

No these are not those type of caps, they don't snap or anything like that. They are a larger diameter than the tubes and have thin ridges on the inside to hold it against the glass tube while leaving a big gap for air exchange


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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583151 - 08/27/16 11:01 AM (7 years, 5 months ago)

Quote:

globejs said:
Actually that's not true. Watch the chapter on master slants on youtube. At exactly 3.5 minutes in he says, "now because of the fact that  we screwed the lids down tight we don't want to do anything to speed up the cool down process or you'll end up exploding your test tubes."




Hmm I'm gonna need to dig my copy out and watch it again. But I personally would not do a slant that way.


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Offlineglobejs
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Re: Micropore tape for PCing master slant tubes? [Re: Pastywhyte]
    #23583362 - 08/27/16 12:20 PM (7 years, 5 months ago)

Well I went to the store, bought a roll of wide micropore tape, and wrapped the seam up with about 2 or 3 layers after filling with agar and a popsicle  stick. I just turned off the pc after 25 minutes and I'm cooling it down au naturale. Guess we will see what happens.


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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583363 - 08/27/16 12:21 PM (7 years, 5 months ago)

That sounds like it's gonna be a pain. . .

I would have just put them in a jar with a filtered lid and left the caps on loose.


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Offlineglobejs
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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583387 - 08/27/16 12:32 PM (7 years, 5 months ago)

I'm surprised  that nobody had any knowledge about these caps or taping, they are the only tubes that one of the biggest supply sponsors sells. That's why I figured they might be fine. I think I need some parafilm in the near  future for long term storage


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Offlineglobejs
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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583394 - 08/27/16 12:34 PM (7 years, 5 months ago)

A pain to remove the tape to get into the tube? Probably...


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Re: Micropore tape for PCing master slant tubes? [Re: globejs]
    #23583456 - 08/27/16 01:04 PM (7 years, 5 months ago)

yeah, micropore gets kinda gross when it's wet. Leaves a lot of adhesive behind.

I always try and do my slants in a jar with a filter like pasty said.

I had a couple that were too big for jars, so I sat them upright, kept them a touch loosely capped, and topped them with foil during the PC cycle. It worked fine, only melted a bit of the texture on the outside of the lids, but they were otherwise fine.


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OfflineRooster Cogburn
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Re: Micropore tape for PCing master slant tubes? [Re: dankington]
    #23583517 - 08/27/16 01:22 PM (7 years, 5 months ago)

I PC my tubes with the cap on tight, I learned that in TMC.
I have also done it leaving them loose, but in the end leaving them tight is easier, when the cycle is over and PC cooled just pull the rack and put it an angle to cool and that's it. I have never seen an exploded tube.


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Re: Micropore tape for PCing master slant tubes? [Re: Rooster Cogburn]
    #23583528 - 08/27/16 01:24 PM (7 years, 5 months ago)

I have cracked a few from tightening them up.


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