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Offlineshroombasa
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Re: Advices needed for commercial grow [Re: Chk]
    #23828405 - 11/13/16 09:33 AM (7 years, 2 months ago)

Endospores are more vulnerable to heat treatment after a soak, although even then it may take a long time with atmospheric steam.  You can use pressure to heat your core up to 121C for 2 hours or you can do a lesser heat for a longer period (i.e. 100C at 12 hours).  Sterilization is a spectrum, but the bare minimum temperature you need is around 90C if I remember correctly from GGMM.  Regardless of theory, there are respected cultivators on here use fractional sterilization with high degrees of success.


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OfflineFerather
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Re: Advices needed for commercial grow [Re: shroombasa]
    #23828450 - 11/13/16 09:49 AM (7 years, 2 months ago)

I agree, and I am beginning to think DIY spawn would be the next logical step.

I can add whole grain flour to water, it is then much easier to clean.
So I sterilize my slurry, needing less time, and add it to body.

Any body will do, but paper pellets are a good start.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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OfflineGr0wer
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Re: Advices needed for commercial grow [Re: Ferather]
    #23828704 - 11/13/16 11:10 AM (7 years, 2 months ago)

So drake, your saying i cant do multiple generations of G2G with steam sterilization?


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OfflineChk
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Re: Advices needed for commercial grow [Re: Gr0wer]
    #23829051 - 11/13/16 12:41 PM (7 years, 2 months ago)

Normally, real sterilization is achieved in 24h at 121c, 
i guess he meant that 24h at 100c leave too many endospores alive to make decent master jars.


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Edited by Chk (11/13/16 12:47 PM)


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OfflineChk
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Re: Advices needed for commercial grow [Re: shroombasa]
    #23829061 - 11/13/16 12:43 PM (7 years, 2 months ago)

Quote:

Sterilization is a spectrum, but the bare minimum temperature you need is around 90C




That's the number i wanted to know;)


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Offlinedrake89
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Re: Advices needed for commercial grow [Re: Gr0wer]
    #23829137 - 11/13/16 01:02 PM (7 years, 2 months ago)

Quote:

Gr0wer said:
So drake, your saying i cant do multiple generations of G2G with steam sterilization?



Not unless you like throwing out a lot of spawn...steam is not sufficient to treat grain spawn without a much higher contamination rate than pressure treatment.


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Offlineshroombasa
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Re: Advices needed for commercial grow [Re: drake89]
    #23830263 - 11/13/16 07:36 PM (7 years, 2 months ago)

I could've sworn Solarity does his grain spawn with fractional sterilization.  Maybe he will chime in!  (Please!)


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Offlinedrake89
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Re: Advices needed for commercial grow [Re: shroombasa]
    #23830339 - 11/13/16 08:01 PM (7 years, 2 months ago)

Quote:

shroombasa said:
I could've sworn Solarity does his grain spawn with fractional sterilization.  Maybe he will chime in!  (Please!)



like i said before you can expand your last generation on steam treated grain, but doing your masters without pressure cooking is asking for trouble.  unless you're doing tyndalization, which isn't exactly practical for most people since it ties up your vessel for at least 4 or 5 days.


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Offlineshroombasa
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Re: Advices needed for commercial grow [Re: drake89]
    #23830631 - 11/13/16 09:34 PM (7 years, 2 months ago)

Makes sense and I agree completely.  I was more curious what people see as far as a success/contam rate.


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Offlinefrog48
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Re: Advices needed for commercial grow [Re: Chk]
    #23831109 - 11/14/16 03:44 AM (7 years, 2 months ago)

Too risky IME to do several generations. G1 and G2 PC and G3 in my wood fired steam sterilizer.


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Offlinefrog48
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Re: Advices needed for commercial grow [Re: frog48]
    #23831198 - 11/14/16 05:01 AM (7 years, 2 months ago)

This is if you make your own spawn. I'm doing mainly shiitake, so may be with oysters you can get away with it because the myc is far more agressive than shiitake. Certainly if you buy your G1 spawn at a good spawn producer, if I'm not mistaken that's what Solarity does.


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OfflineFerather
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Re: Advices needed for commercial grow [Re: frog48]
    #23831435 - 11/14/16 07:58 AM (7 years, 2 months ago)

:takingnotes:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineChk
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Re: Advices needed for commercial grow [Re: frog48]
    #23831508 - 11/14/16 08:37 AM (7 years, 2 months ago)

Not making my own spawn would take out a good part of the fun ^^


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