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Invisiblec10h12n2o
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Back in the Saddle / AA+ Pin Porn
    #23560351 - 08/20/16 04:02 PM (7 years, 6 months ago)

hello lovely people :smile: I had taken a break from the hobby for a while and now have begun practicing (rather than just lurking and reading) again in earnest this summer.

Most of my recent work has been on agar, doing isolations on several races and species. After several series of transfers i made LIs from some of the sexiest plates and started making spawn with these, making them somewhat limited MS

Out of everything, the AA+ has shown the most rhizo growth and been the fastest, so much so that it got put in fruiting conditions more than a full week before other tubs created the same day.  wheat berries spawned to hpoo, coir, verm, straw, and gypsum.

Here are some pics from today, looking like it is going to be a nice pinset, covered in hyphal knots :grin:




covered in these:

Get Hyphy!


I have never worked with AA+ before, and it has been a couple of years since i have been involved with practicing the hobby. Does everything look alright? any suggested intervention, or any other tips about proceeding from here? I have been REALLY lucky with my monotubs in the past but i am trying to dial in my technique before i start testing all these isolates and would appreciate any feedback

thanks everyone! :smile:

ps: i have some really exciting spawn and plates going right now, including p. micro. (polyurethane digesting fungus), cordyceps militaris , pan tropicalis (pan cyan?), reshi,  and a ridiculously fast PE6 culture :smile: and bulk substrate colonizing for lions mane, shaggy mane, blue oyster buckets, pheonix/lung oyster buckets, and many more! should be a busy summer!! :laugh:


--------------------

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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Invisiblemorty422
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23560480 - 08/20/16 04:36 PM (7 years, 6 months ago)

Looking good man!

I'm in no way the most skilled cultivator here. But I would say you're on the right track.

Looks.like you have a lot of specimens rolling. Should be a really fun time!

Oysters are my area of experience... I would love to see you post some updates when you get those into spawn.

Nice job man. Keep us updated when that tub explodes!

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InvisiblePastywhyteMDiscord
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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
    #23560536 - 08/20/16 04:57 PM (7 years, 6 months ago)

Looks great :rockon:

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
    #23560653 - 08/20/16 05:37 PM (7 years, 6 months ago)

thanks man!  :highfive1: 


I am having a lot of fun with it, learning lots :smile: thanks in no small part to this wonderful community. I am really excited to play around with all these isolates, and harvest/clone the MS in the meantime. I have some shaggy mane and lions mane colonizing bulk right now, my first time with both, looking great so far though! lions mane had pins in the grain jars!

i would love some feedback on the oysters too, since its my first experience with them as well, would be great to have someone experienced handy :smile: I have 1 phoenix/lung oyster bucket going right now that might be ready to fruit, im not really sure  :shrug:  and i have 2 smaller blue oyster buckets that have been colonizing for 5 days, probably about time to check on them (though i kinda have low expectations for them despite the beautiful grainspawn, because my dumb ass spilled the spawn on concrete while it was draining on the window screen after soaking the grains a la Frank, i salvaged what seemed clean , but my expectations arent high considering this. needless to say, i got a rope instead of rubber bands to hold the screen from now on :/ what would you like pics of? i have documented most of the process. any feedback is much appreciated :smile:

thanks pasty!! you definitely deserve some of the credit :wink: cant thank you enough for everything you do around here


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23563066 - 08/21/16 01:38 PM (7 years, 6 months ago)

some pics from this morning:



there are actually way more pins and hyphal knots that can be seen in the pics, had trouble finding a spot without condensation to take pics from. This is my first experience with AA+, its so crazy how lightly colored the pins are, so cool :smile:


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Invisiblemorty422
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23563072 - 08/21/16 01:41 PM (7 years, 6 months ago)

:hellyeah:

Pins!!!

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InvisiblePastywhyteMDiscord
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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
    #23563086 - 08/21/16 01:49 PM (7 years, 6 months ago)

Just take the lid off to snap a clear picture. Won't hurt a thing.

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Invisiblemorty422
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Re: Back in the Saddle / AA+ Pin Porn [Re: Pastywhyte]
    #23563167 - 08/21/16 02:43 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Just take the lid off to snap a clear picture. Won't hurt a thing.




:whathesaid:

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: Pastywhyte]
    #23563244 - 08/21/16 03:23 PM (7 years, 6 months ago)

thanks for the tip my pasty brother, here are some better shots with the lid off :wink: still dont stick out as much in photos since its leucistic(sp?) but im stoked about all the hyphal knots and tiny pins still starting :smile: this is my first tub in a while, so if theres anything i should do to make the most of it i am all ears :smile:







I have a BUNCH of stuff in the works, should be a very busy summer :smile: cant wait to start testing all these isolates i have been working on, and the cordyceps, and the reishi, lions mane, shaggy mane, lots of oysters, and the p. micro (plastic digesting) :laugh: really want to dial in my techniques and sharpen up before i start testing isolates

thanks again everyone!


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23566108 - 08/22/16 02:08 PM (7 years, 6 months ago)

here are some shots from this morning :grin:



coming along beautifully :smile: its my first albino/leucistic race so im amazed at how crazy looking they are! so cool :smile:

btw, quick question:
I plan to take a few clones from this tub. I have successfully cloned mature mushrooms the 5-6 times ive tried, but i have been reading a lot of people saying that if you are going to take a clone you should do it early, youngest myc possible, ideally one of the first pins (i have no idea about this). Should i wait until the fruits mature and showing characteristics and clone the ones that are most interesting, or should i clone some of these first flush pins now?

thanks again everyone


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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InvisiblePastywhyteMDiscord
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23566129 - 08/22/16 02:16 PM (7 years, 6 months ago)

Clone a pin from a cluster. Clone a few really. Cluster genetics often produce way better and the young pin will produce a culture with more vigor.

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InvisibleSuperFly
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23566150 - 08/22/16 02:24 PM (7 years, 6 months ago)

get hyphy hahaha you're awesome

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: SuperFly]
    #23566238 - 08/22/16 02:56 PM (7 years, 6 months ago)

Quote:

Pastywhyte said:
Clone a pin from a cluster. Clone a few really. Cluster genetics often produce way better and the young pin will produce a culture with more vigor.




thanks pasty, makes lots of sense, you da man :rockon:

are there any special precautions i should take when removing pins to clone (regarding the selected pin or the rest of the tub)? or just pluck em out with gloved fingers and clone as usual (dip in peroxide, biopsy inside center of stipe, transfer to agar)? or is there a better technique using scalpel or anything else? never cloned a pin before, only mature fruits

Quote:

SuperFly said:
get hyphy hahaha you're awesome




lol stay hyphy like keak da sneak my brother


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23569030 - 08/23/16 12:35 PM (7 years, 6 months ago)

ok so i made some clones with flame sterilized tweezers and scalpel a la RR's thread, and i have never seen such deep blue bruising :laugh: maybe its just because of the light colored fruits, but it looked like ink!! hopefully i didnt fuck anything else up too bad, might have caused one cluster's babies to abort



--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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InvisiblePastywhyteMDiscord
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23569066 - 08/23/16 12:52 PM (7 years, 6 months ago)

If ya wanna make an omelet. . .

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: Pastywhyte]
    #23572692 - 08/24/16 12:24 PM (7 years, 6 months ago)

im tryin to make one paula deen style, with butter and sour cream  :derpyum:



lol so yeah, i was worried about the couple of pins i might have touched and causing them to abort, looks like i have a much bigger problem, tons of aborts today!!!

i knew all those knots looked a little too good to be true, considering the unlevel substrate and MS spawn, and the fact that its my first tub in quite a while

the clones i took are looking good. Someting really cool though, several of the pins have aborted have gotten super fuzzy and thick at the bottom, like trying to re absorb. and the ones near large fruits are absorbing into the stems of the big ones

is there anything i should do about these at this point? any intervention called for to make the most of the situation? i have lots more tubs in the works, so its not like i am banking on this one or anything, just trying to get some good clones and play around with this race, and most of all learn :laugh:



thanks again everyone!! best regards


PS edit:  I have another 10 jars of AA+ spawn ready to go today. they were made using LI from limited MS plates, even more limited than the first one because it was further along in the same series of transfers. I am trying to decide between just doing another 2-3 monotubs with them or putting them into trays (plastic 5qt shoeboxes) and fruiting them in a GH.

my main objective for these is just to obtain some good clones and sharpen up my technique before i start my serious projects, testing all these isolates i have been working on. I have 6 other races of MS colonizing in tubs right now, all for the same purposes. so ideally i would like to be practiced up to refine and standardize the methods i will be using to test all the important stuff. in light of what i am tryin to do, what makes the most sense?


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Edited by c10h12n2o (08/24/16 12:43 PM)

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OfflineRooster Cogburn
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23572863 - 08/24/16 01:38 PM (7 years, 6 months ago)

Very nice:super:
Looking beautiful, as much as I want to kick back and watch you guys grow these magnificent beasts, I need to get on this AA+ train.

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: Rooster Cogburn]
    #23573067 - 08/24/16 02:44 PM (7 years, 6 months ago)

i'll PM you good buddy :wink:


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Invisiblec10h12n2o
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23579544 - 08/26/16 11:24 AM (7 years, 6 months ago)

UPDATE:

harvested yesterday, all but a couple that i will harvest today

total wet weight harvested out of this first flush so far is 1097g

cant wait to start working with clones and isolates :laugh:




--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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OfflineRooster Cogburn
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
    #23579786 - 08/26/16 12:25 PM (7 years, 6 months ago)

:super:Fuck yeah
Fill your hands with that you son of a bitch!

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