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Offlinejay_swan
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Registered: 10/29/15
Posts: 4
Last seen: 5 years, 11 months
Problems with pf-tek start up grow
    #23576104 - 08/25/16 01:28 PM (7 years, 5 months ago)

Hello everyone,

I am in need of a helping hand understanding my poor grow results lately. I grow cubensis and have been having 0 harvests in the last 3 batches of 6 jars!!
I have accomplished many successful grows but right now, I dont even have any lifeforms inside my jars except for one little spot in the last jar inoculated!
So im pretty sure the sterility part is done right but it's the part where the syringe goes in that I don't understand. I have done everything right I believe :

1. I place my jars inside a aluminum pot and wash everything with Lysol.
2. Place my little torch lighter and the syringe inside the pot
3. Remove the aluminum foil outside the pot and immediately wipe top surface with a Lysol cloth
4. Immediately flame sterilize my 1 1/2 inch 18 gauge needle
5 Push a little water to cool down the needle
6 Insert to about 1/4 away from dry matter and inject half a cc into a hole ( I have four holes, all open to the air.
7 Wipe again with a dry bounty.
8 Store in the right place.

So what do you guys think?! I dont see where my problem is and I am very grateful for any scrap of a hint as to what is going on


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OfflineItalianKillbasa
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Registered: 03/03/16
Posts: 27
Last seen: 1 year, 4 months
Re: Problems with pf-tek start up grow [Re: jay_swan]
    #23576118 - 08/25/16 01:34 PM (7 years, 5 months ago)

Anything else growing, like contaminates?  Maybe you have a weak spore syringe?


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Offlinejay_swan
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Registered: 10/29/15
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Last seen: 5 years, 11 months
Re: Problems with pf-tek start up grow [Re: ItalianKillbasa]
    #23576144 - 08/25/16 01:45 PM (7 years, 5 months ago)

Nothing at all!!! The ones I had before never grew contaminants after 2 weeks and I had one detail syringe and one I had made myself ( and trust me, I use at least half a big cap spore print to make my home brews), and maybe im wrong but spore prints have no expiry date right?


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Problems with pf-tek start up grow [Re: jay_swan]
    #23576360 - 08/25/16 02:59 PM (7 years, 5 months ago)

Spore prints definitely have an expiry date.


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InvisibleKenInVic
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Registered: 03/01/16
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Re: Problems with pf-tek start up grow [Re: Pastywhyte]
    #23576385 - 08/25/16 03:08 PM (7 years, 5 months ago)

Quote:

Pastywhyte said:
Spore prints definitely have an expiry date.




How long are we talking and is it dependent on how they're stored, Pasty?


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OfflineItalianKillbasa
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Re: Problems with pf-tek start up grow [Re: KenInVic]
    #23576434 - 08/25/16 03:22 PM (7 years, 5 months ago)

I've read you can store in the fridge for about a year.  I would use Pasty's Agar tek and see if you can grow anything on one with your syringe just to test it!!  I once inoculated 12 jars with a syringe and only had 2 actually grow something...shit happens!

Ahh I'm dumb I read spore syringes for some reason...yeah I don't know about spore prints but I've used one that was about 6 months old and had decent grows from it.  Now I wonder how long I have left on the rest of my spore prints?!?


Edited by ItalianKillbasa (08/25/16 03:25 PM)


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Offlinejay_swan
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Registered: 10/29/15
Posts: 4
Last seen: 5 years, 11 months
Re: Problems with pf-tek start up grow [Re: ItalianKillbasa]
    #23576509 - 08/25/16 03:38 PM (7 years, 5 months ago)

Quote:

I've read you can store in the fridge for about a year.  I would use Pasty's Agar tek and see if you can grow anything on one with your syringe just to test it!!  I once inoculated 12 jars with a syringe and only had 2 actually grow something...shit happens!

Ahh I'm dumb I read spore syringes for some reason...yeah I don't know about spore prints but I've used one that was about 6 months old and had decent grows from it.  Now I wonder how long I have left on the rest of my spore prints?!?




Hahahaha well I have just bought some brand new syringes but how long can the prints last before becoming unviable for use???


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Problems with pf-tek start up grow [Re: KenInVic]
    #23576701 - 08/25/16 04:37 PM (7 years, 5 months ago)

Quote:

KenInVic said:
Quote:

Pastywhyte said:
Spore prints definitely have an expiry date.




How long are we talking and is it dependent on how they're stored, Pasty?




Between 3 to 8 years. Depends on storage and the genetics of the lineage.


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Offlinejay_swan
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Registered: 10/29/15
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Last seen: 5 years, 11 months
Re: Problems with pf-tek start up grow [Re: Pastywhyte]
    #23576721 - 08/25/16 04:43 PM (7 years, 5 months ago)

Well then cant be genetics because they are only 8-10 months old so I'm back to square one!! any other ideas??


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Problems with pf-tek start up grow [Re: jay_swan]
    #23577022 - 08/25/16 05:54 PM (7 years, 5 months ago)

Maybe your substrate is too dry? It's hard to say without seeing the jars. Let's see some pics.

All the lysol and bounty in the world won't help you with sterility if you're inoculating in open air and steaming your jars. Consider using a pressure cooker and a still air box. While you can scrape by with the PF tek without them it's just not good practice if cultivation is something you're going to be doing for awhile.


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