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Offlinefungi-funguy
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Registered: 02/13/16
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Do you prefer: Print 2 agar or Print 2 syringe 2 agar??
    #23574475 - 08/24/16 10:57 PM (7 years, 5 months ago)

So i am barely getting into agar ( I tried using Pastys Agar Tek in the past but ended up melting the agar dishes in the PC :eek: ) And this is my first time using prints too!

Do you prefer, Print 2 agar or Print 2 syringe 2 agar?? Or any other method that you prefer and why?


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InvisibleTuhdoww
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Registered: 08/23/16
Posts: 300
Re: Do you prefer: Print 2 agar or Print 2 syringe 2 agar?? [Re: fungi-funguy]
    #23574479 - 08/24/16 10:59 PM (7 years, 5 months ago)

If the prints are super dry they will take longer to germinate. With syringes your spore are definitely hydrated prior to inoculation.


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Offlinefungi-funguy
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Re: Do you prefer: Print 2 agar or Print 2 syringe 2 agar?? [Re: Tuhdoww]
    #23574485 - 08/24/16 11:01 PM (7 years, 5 months ago)

Okay cool thanks. And wait like 24 hours before using syringe right?


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InvisibleTuhdoww
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Registered: 08/23/16
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Re: Do you prefer: Print 2 agar or Print 2 syringe 2 agar?? [Re: fungi-funguy]
    #23574490 - 08/24/16 11:04 PM (7 years, 5 months ago)

That would be sufficient. Currently on my first pasty plates. Used spores that were in my drying chamber for weeks. I use calcium chloride, sucks up all available moisture from everywhere, and my prints were bone dry. Just starting to see some action after more than a week


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Offlinefungi-funguy
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Re: Do you prefer: Print 2 agar or Print 2 syringe 2 agar?? [Re: Tuhdoww]
    #23574511 - 08/24/16 11:11 PM (7 years, 5 months ago)

Damn a week? so if i went directly from print 2 agar it would take longer but it would minimize the risk of contaminating from getting them in a syringe first.. what to do what to do..


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Offlinetump
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Registered: 03/17/16
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Re: Do you prefer: Print 2 agar or Print 2 syringe 2 agar?? [Re: fungi-funguy]
    #23574596 - 08/24/16 11:48 PM (7 years, 5 months ago)

Print to agar is the best method. Look up tiger drop.


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Invisibleweetsie
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Registered: 05/08/11
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Re: Do you prefer: Print 2 agar or Print 2 syringe 2 agar?? [Re: tump]
    #23574732 - 08/25/16 12:45 AM (7 years, 5 months ago)

Spores print > agar every time unless you have a specific reason to do otherwise.


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