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InvisiblePastywhyteMDiscord
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Re: Anastomosis [Re: Boogieman47]
    #23675578 - 09/24/16 11:53 PM (7 years, 4 months ago)

Because having the psoibily of monokaryns anastomsing or mating together is better for the purpos of creating a varital cross. You can't see it at all unless under a scope.


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InvisibleInocuole
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Re: Anastomosis [Re: Pastywhyte]
    #23675580 - 09/24/16 11:55 PM (7 years, 4 months ago)

I like how when you get drunk you don't necessarily get any worse at explaining stuff succinctly. :lol:


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InvisiblePastywhyteMDiscord
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Re: Anastomosis [Re: Inocuole]
    #23675584 - 09/24/16 11:58 PM (7 years, 4 months ago)

Lol yer right righttn

K I'm dune. Gnithth


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InvisibleInocuole
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Re: Anastomosis [Re: Pastywhyte]
    #23675586 - 09/24/16 11:59 PM (7 years, 4 months ago)

Thanks for popping in here dude.  :thumbup:  Will wait til later to ask some specific questions after I brew on em for a bit.


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InvisibleBoogieman47
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Re: Anastomosis [Re: Inocuole]
    #23676747 - 09/25/16 01:13 PM (7 years, 4 months ago)

Haha i was trippin last night trying to make out the text and figured i would also wait to respond ... so it would probably be best to use a microscope when trying to cross? Unlesd you just have to guess on cuts


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InvisibleNumeroEno
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Re: Anastomosis [Re: Boogieman47]
    #23676824 - 09/25/16 01:43 PM (7 years, 4 months ago)

Quote:

noob47 said:
How come taking fresh spores is the best way rather then mixing colonized spawn? Is it just because you can see the growth better that way?




Colonized spawn consists of already dikaryotic mycelium. If you mix two different types of colonized spawn and fruit it, you'll just get two different types of mushrooms. Try putting transfers from two different cultures on the same plate and watch what happens when they grow against each other. The same thing happens if you mix two different types of spawn.


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InvisibleBoogieman47
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Re: Anastomosis [Re: NumeroEno]
    #23676842 - 09/25/16 01:51 PM (7 years, 4 months ago)

So basically once the spores germinate, then trying to get them to fuse is like sticking a square through a circle??... so anastomosis is where they come together?


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InvisiblePastywhyteMDiscord
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Re: Anastomosis [Re: Boogieman47]
    #23676867 - 09/25/16 01:58 PM (7 years, 4 months ago)

If you get enough different strains on converging paths eventually it's likely some will be able to fuse and many should be able to fruit together. Serial dilutions and monokaryons are handy but not completely required for a cube ir varietal cross. If you can use fresh spores it will be likely some monokaryons will mate as well.


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InvisibleBoogieman47
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Re: Anastomosis [Re: Pastywhyte]
    #23676993 - 09/25/16 02:49 PM (7 years, 4 months ago)

Ok that makes sense im swabbing my plate tonight


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InvisibleDuke of disjour
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Re: Anastomosis [Re: Inocuole]
    #23677692 - 09/25/16 07:13 PM (7 years, 4 months ago)

+5


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Offlinecatnip40
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Re: Anastomosis [Re: Duke of disjour]
    #23682309 - 09/27/16 06:51 AM (7 years, 4 months ago)

:takingnotes:


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Invisibledankington
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Re: Anastomosis [Re: catnip40]
    #23701627 - 10/02/16 08:57 PM (7 years, 3 months ago)

So, some of us were talking on another forum about how mycelium can intertwine and share characteristics; eg. Mycogone growing from cube substrate can bruise, and the oysters that invaded Pussyfart's cubensis cakes would bruise (but weren't active).

Is this all anastomosis of different species? Just how much of these characteristics can actually be shared, I wonder. Considering that an invading mold can take on the bruising characteristic when growing on Cubensis mycelium, I find that really amazing. Mad Season speculated it was from the digestion of the cubensis mycelium by the invading mycelium, which seems completely plausible. But say in the case of the oysters, their bluing didn't make them active at all (if bluing were to actually be deterioration of Psilocin, which we know is probably not true).

I don't really have a proper question formed just yet. Just thinking out loud here.
:bongload:

edit: goddamn it. Both PF and Muda got oyster infested cubes, but Muda's bruised. Sorry for the mixup.


Edited by dankington (10/03/16 07:26 AM)


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InvisiblePastywhyteMDiscord
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Re: Anastomosis [Re: dankington]
    #23701639 - 10/02/16 09:00 PM (7 years, 3 months ago)

One thing about white molds is that the nasty disgusting blobs they produce are indeed cube fruits. That's why they bruise blue. You can eat them and trip but it's nasty shit.


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InvisibleInocuole
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Re: Anastomosis [Re: Pastywhyte]
    #23702354 - 10/03/16 03:11 AM (7 years, 3 months ago)

I don't think the absorption of the bluing mechanism is a sign of anastomosis.  If anything it's just a sign that the competing colony was able to absorb some of the enzymes and chemicals produced by the mycelium.

Also, it was Muda whose oysters turned blue.  So now you've gone and credited PF for that in multiple threads. :lol:


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Re: Anastomosis [Re: Inocuole]
    #23702602 - 10/03/16 07:01 AM (7 years, 3 months ago)

:smbfacepalm:
I guess I got a little mixed up. PF did just get oysters in with his cubes, didn't mention the bluing
https://www.shroomery.org/forums/showflat.php/Number/23205057#23205057

Here's muda's blue oysters


Edited by dankington (10/03/16 08:38 AM)


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Invisiblefunkyfish77
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Re: Anastomosis [Re: dankington]
    #23705896 - 10/04/16 07:17 AM (7 years, 3 months ago)

Well I thought I would get a few pics before I start cloning and cutting this plate up. The big section with all the little pins is a crs culture that's pretty old and all it does anymore is pin. The small section is a lucy crs/aa+ f4 cross not rust spored that came apart . I'm planning on cloning the big pins on the party line and see what they are .I like waiting on the plates to pin then look for hybrid vigor on the party lines.


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InvisibleMycolorado
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Re: Anastomosis [Re: Inocuole]
    #23846515 - 11/18/16 08:14 PM (7 years, 2 months ago)

Couple colonies from syringe.  Anastomosis over the zone of inhibition?


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