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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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wormpoo
Stranger

Registered: 01/27/16
Posts: 5
Last seen: 6 years, 1 month
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Hi, Malachite green (Aquarium shop) and Lactophenol blue (microscope outlets) are both pretty cheap and excellent at staining chitin, the major element in fungal cell walls. Problem is they most probably kill the mycelium at a staining strength!, dark field microscopy could be good for live mycelial growth if you can apapt your scope. I've read somewhere about mycelium being grown in a medium on a slide itself/ between slides? can't remember keep up the good work!
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Anastomosis [Re: wormpoo]
#23615121 - 09/05/16 03:25 PM (7 years, 4 months ago) |
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I can't remember if what I wrote about was from TMC or GGMM. I lent my copy of TMC to a friend, and I'm house sitting so away from my copy of GGMM, but next time I go home I'll see if I can post an excerpt.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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I just read GGMM cover to cover and don't remember seeing anything about individual spores. just an fyi
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Praise him.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Im in on this wanna read more but busy
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 9 hours, 19 minutes
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-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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ive been trying to learn more about this lately, since i am fascinated by the dynamics of strain organization and interaction in a multi-strain culture. and any genetic exchange at this point in the lifecycle is damn interesting
i was about to throw away a few hundred plates, so i went through them individually to look for anything of interest, and noticed a particular variety has a strong propensity towards developing that crackle looking pattern i posted a pic of earlier in the thread. it has mainly been that same race (AA ), and it takes a while to start developing
I was able to find about a dozen examples, and will photograph them shortly and post pics for talking points. soon i will have a microscope set up to take time lapse videos, and maybe i will be able to catch it during formation
i dont know how much or if it is indeed anastomosis involved, but it seems likely. at first i thought it must be some kind of reaction to bacteria, but the plates seem clean... idk
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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let this one go a bit too long is this anastomosis?
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Nah anastomosis is the process of monokaryotic cultures mating, or in some cases, dikaryotic isolates mating to form a third culture. RR did that with rattlesnake venom to produce PE6. You can't see it happen without a microscope, unless you do it like RR did and a third sector appears at the point where the 2 single sector isolates meet. What you have there is the mycelium that gives way to fruit body formation (the stuff that looks kind of like chain lightning). The anastomosis all happened when the individual monokaryons mated right after the spores germinated.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Oh ok cool everything about this is interesting as fuck i doubt ill ever get that deep into it but just learning everyday new shit is cool and i really enjoy it
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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You seem to be pretty serious about cultivation and a quick learner. Definitely check out the PDF of the Stamets book I posted. That will teach you a lot. You'll be surprised how much information you'll absorb just from being active on the forum.
Anyway, if you want to do your own anastomosis experiment, take a swab of PE, take a swab, of a regular cube, streak them over each other on the same plate, and take a transfer from the last part of your streak. There's a chance you'll get a hybrid that way. It's been done before. You can also do it with a serial dilution of spore solution. If you dilute it enough you can get monokaryotic cultures and mate them on a new plate.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Ya im taking my star gazers and pe to some tgis weekend i will read that i appreciate i have to drive a few hours will talk later friend
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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To be fair there probably is some anastomosis happening in that plate but as Eno said we can't see it without a scope.
My trials with rustywhyte have all depended on anastomosis to some degree.
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Quote:
NumeroEno said: You seem to be pretty serious about cultivation and a quick learner. Definitely check out the PDF of the Stamets book I posted. That will teach you a lot. You'll be surprised how much information you'll absorb just from being active on the forum.
Anyway, if you want to do your own anastomosis experiment, take a swab of PE, take a swab, of a regular cube, streak them over each other on the same plate, and take a transfer from the last part of your streak. There's a chance you'll get a hybrid that way. It's been done before. You can also do it with a serial dilution of spore solution. If you dilute it enough you can get monokaryotic cultures and mate them on a new plate.
How would this be a hybrid? I guess in the sense of strains but not in the sense of species. That is a little more interesting to me but I don't have any experience myself. Some interesting literature out there tho.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Anastomosis [Re: drake89]
#23674870 - 09/24/16 06:49 PM (7 years, 4 months ago) |
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It wouldn't be a species hybrid but cube hobbiests refer to varietal crosses as hybrids as do cube spore vendors. It's technically incorrect nomenclature but such things are slow to change in our hobby.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Crossing 2 varieties of landrace Cannabis indica is also called a hybrid, for example.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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I think as long as you specify whether it's a "strain" or interspecies hybrid, it negates the need to find a new word.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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How come taking fresh spores is the best way rather then mixing colonized spawn? Is it just because you can see the growth better that way?
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