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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Can you use this for a flowhood?
#23572746 - 08/24/16 12:44 PM (7 years, 5 months ago) |
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Should've just used the search engine first for this sorry guys
But while anyone is here has anyone just steamed their lc and been successful?
Edited by spore-ty (08/24/16 01:02 PM)
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AutistStick



Registered: 06/14/18
Posts: 15
Loc: Canada
Last seen: 6 months, 6 days
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Re: Can you use this for a flowhood? [Re: spore-ty]
#25369324 - 08/06/18 01:44 AM (5 years, 5 months ago) |
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I have tried steam sterilizing 2 LC's in the past and neither of them were successful. In one of them I injected spore solution, in the other I inserted a sliver from the inside of a freshly picked mushroom. Neither grew. after about 2-3 weeks with no growth whatsoever, I tossed them. I am currently making a second attempt at liquid cultures as we speak now that I have a PC - which I strongly recommend getting one if you don't already have one.
Hopefully this info helps you in some way/shape/form. GL with your future endeavors. Peace
--------------------
"Hehehe fuck it dude!" - Paul Denino Cx in the chat.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Can you use this for a flowhood? [Re: AutistStick]
#25369428 - 08/06/18 04:48 AM (5 years, 5 months ago) |
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You LCs failed because you did them the wrong way, I wouldn't blame the steam sterilization. I would blame the improper inoculations
And yes theoretically you could steam sterilize a LC but why would you even bother adding the LC step. Slow things down to speed things up but also add a huge contamination risk. Yeah...
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: Can you use this for a flowhood? [Re: bodhisatta]
#25369464 - 08/06/18 05:45 AM (5 years, 5 months ago) |
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Quote:
bodhisatta said: You LCs failed because you did them the wrong way, I wouldn't blame the steam sterilization. I would blame the improper inoculations
And yes theoretically you could steam sterilize a LC but why would you even bother adding the LC step. Slow things down to speed things up but also add a huge contamination risk. Yeah...
Yeah.. LCs are always a mystery.Quote:
bodhisatta said: You LCs failed because you did them the wrong way, I wouldn't blame the steam sterilization. I would blame the improper inoculations
And yes theoretically you could steam sterilize a LC but why would you even bother adding the LC step. Slow things down to speed things up but also add a huge contamination risk. Yeah...
My mom always told me, LC is like box of chocolates, you never know, what you gonna get.
Edited by mushhiehunter (08/06/18 05:46 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Can you use this for a flowhood? [Re: mushhiehunter]
#25369680 - 08/06/18 08:52 AM (5 years, 5 months ago) |
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Or you inoculate it with Agar
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: Can you use this for a flowhood? [Re: bodhisatta]
#25369697 - 08/06/18 08:59 AM (5 years, 5 months ago) |
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Quote:
bodhisatta said: Or you inoculate it with Agar
What do you mean by or? Is there another way?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Can you use this for a flowhood? [Re: mushhiehunter]
#25369704 - 08/06/18 09:00 AM (5 years, 5 months ago) |
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The dude I originally replied to did biopsy and spores right to LC which is asking for fail
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: Can you use this for a flowhood? [Re: bodhisatta]
#25369782 - 08/06/18 09:48 AM (5 years, 5 months ago) |
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Quote:
bodhisatta said: Or you inoculate it with Agar
My preferred method
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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LetsGetSchwifty
Unorthodox Experimenter


Registered: 07/24/18
Posts: 244
Loc: Underground
Last seen: 2 years, 4 months
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Re: Can you use this for a flowhood? [Re: bodhisatta]
#25370078 - 08/06/18 12:19 PM (5 years, 5 months ago) |
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Quote:
bodhisatta said: The dude I originally replied to did biopsy and spores right to LC which is asking for fail
What about taking a tiny bit of healthy, colonized, non contaminated rice cake for LC?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Would strongly suggest not doing that. Cakes are only steam sterilized, they have a very barrier, they're also inoculated with spores. So it's like doing spores to LC but an extra step to attain failure.
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