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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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6 days since inoculation no sign of colonization
#23569541 - 08/23/16 03:50 PM (7 years, 5 months ago) |
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Hello Shroomery friends. I have a question regarding my rye jars.
i have a few pint jars, and a few quart jars with rye. today i slighty mixed it up to see if there was any mycelium but there wasnt any. do you think it was bad spores? i used MS injection. about 1-3 CC in each jar. Any help would be appreciated.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569554 - 08/23/16 03:53 PM (7 years, 5 months ago) |
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Is that the camera flash or glare or something in those jars?
I see mycelium..
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
#23569558 - 08/23/16 03:54 PM (7 years, 5 months ago) |
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yes camera flash. not mycelium.
Edited by Aos (08/23/16 03:55 PM)
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569562 - 08/23/16 03:55 PM (7 years, 5 months ago) |
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Lol dude are you sure there's not 'white stuff' in your jars?
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
#23569589 - 08/23/16 04:05 PM (7 years, 5 months ago) |
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i am absolutely positive there is no mycelium, i rolled them in my hands for like 5 minutes closely observing. no mycelium. they white you see in the photo is from the camera flash.
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anthiawe
friendly stranger


Registered: 05/18/16
Posts: 652
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569598 - 08/23/16 04:10 PM (7 years, 5 months ago) |
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spore should go to agar not grain i believe. liquid mycelium or colonized agar should go into grain.
-------------------- TEK compendium
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: anthiawe]
#23569606 - 08/23/16 04:12 PM (7 years, 5 months ago) |
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never heard that one before.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569612 - 08/23/16 04:16 PM (7 years, 5 months ago) |
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Spores to agar then agar to mother jar (s) or lc for expansion and you know it's clean so it's all in your aseptic technique
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
#23569625 - 08/23/16 04:22 PM (7 years, 5 months ago) |
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i just bought premade agar petri dishes, im tired of syringes, agar is so much better. should i drop a wedge in those jars anyways? or just dump them out, the wedge will be a different "strain"
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 6 hours
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569637 - 08/23/16 04:26 PM (7 years, 5 months ago) |
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I have inoculated rye berry with a syringe and have had full colonization of mycelium. Its not preferred but it should work.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569644 - 08/23/16 04:29 PM (7 years, 5 months ago) |
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premade agar dishes? You're going to really want to use more than just a couple of dishes. I rotate through ~20-30 weekly, and I'm a very minor grower. If I were serious, I'd be going through hundreds a month! Being able to toss something readily is really nice. You don't want to end up trying to save hopeless specimens. You also want to be able to take several transfers.
Also spores to grain

especially not 1-3cc, that's way too much! Next time, if you must, just try expel just a drop right on the top of the grains.
Also, 6 days is really not enough time to get worried.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
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Re: 6 days since inoculation no sign of colonization [Re: anthiawe]
#23569659 - 08/23/16 04:38 PM (7 years, 5 months ago) |
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Quote:
anthiawe said: spore should go to agar not grain i believe. liquid mycelium or colonized agar should go into grain.
syringes work straight on grain jars, ime. You may not get the growth you want though, which is why I personally started agar. 6 days is nothing. Try 10-14 before you get nervous
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569707 - 08/23/16 04:57 PM (7 years, 5 months ago) |
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how long has been since you inoculated them?
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23569721 - 08/23/16 05:02 PM (7 years, 5 months ago) |
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It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
#23569722 - 08/23/16 05:03 PM (7 years, 5 months ago) |
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Quote:
paracelsusgold said: how long has been since you inoculated them?
Good question Hmmm
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
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Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
#23569727 - 08/23/16 05:04 PM (7 years, 5 months ago) |
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Spores are nitoriously filthy as they can't be harvested under sterile conditions. Like the others have said, avoid using MS to grain directly. Put it on agar first to increase your chances of getting clean spawn.
MS to grain can work, but its bad technique with a low success rate. 7 days isn't very long to wait for spore germination....you can see growth anywhere from 1-3 weeks depending on how hydrated the spores are.
Loving the lid with the Whatman filter. If you decide to stick with the hobby you might want to invest in proper SFDs. Poly is a good budget option but it needs to be stuffed super tight to avoid contamination.
Welcome to the Shroomery brother. Hope you stick around.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17] 1
#23569758 - 08/23/16 05:11 PM (7 years, 5 months ago) |
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6 days means two things....you didn't get any contams quickly and you lack patience lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17]
#23570002 - 08/23/16 06:14 PM (7 years, 5 months ago) |
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: 6 days since inoculation no sign of colonization [Re: cronicr]
#23570024 - 08/23/16 06:20 PM (7 years, 5 months ago) |
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Quote:
cronicr said: 6 days means two things....you didn't get any contams quickly and you lack patience lol
This. Ive had spores take 3 weeks to germinate before. Its rare, but does happen.
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
#23570815 - 08/23/16 09:43 PM (7 years, 5 months ago) |
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 Quote:
kenetic said: It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.
hey ken how are you feeling? if the mycelium has not started growing out after 6 days then there is something wrong and probably the spores were old or no good. one would think that there would be some contamination by then also.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: 6 days since inoculation no sign of colonization [Re: cronicr]
#23570821 - 08/23/16 09:44 PM (7 years, 5 months ago) |
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 Quote:
cronicr said: 6 days means two things....you didn't get any contams quickly and you lack patience lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Re: 6 days since inoculation no sign of colonization [Re: ComebackKid]
#23570905 - 08/23/16 10:02 PM (7 years, 5 months ago) |
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Quote:
ComebackKid said:
Quote:
paracelsusgold said: how long has been since you inoculated them?
Good question Hmmm
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: 6 days since inoculation no sign of colonization [Re: Psychedel.EXE]
#23570984 - 08/23/16 10:19 PM (7 years, 5 months ago) |
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Don't waste the jars like a few of them are saying spore syringes can take weeks to develop, but like dank said that's way too much .5 a cc is all you need I waited 4 weeks before I figured it wasn't happening and started agar in the mean time never knocked up jars with a syringe other then a LI from clean agar .... if you have an Asian mart by you they have agar sticks they are 20 grams for 1.30$ at mine and they are pre colored
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Re: 6 days since inoculation no sign of colonization [Re: Boogieman47]
#23571026 - 08/23/16 10:27 PM (7 years, 5 months ago) |
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OP, you should add that photo to your gallery and edit your first post so it can be enlarged. Its best to let shroomery host the pics. I also vote you try out agar while you wait.
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23571074 - 08/23/16 10:42 PM (7 years, 5 months ago) |
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Quote:
paracelsusgold said:
 Quote:
kenetic said: It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.
hey ken how are you feeling? if the mycelium has not started growing out after 6 days then there is something wrong and probably the spores were old or no good. one would think that there would be some contamination by then also. 
Dont mean to sound rude Parcel but this is just bad info. As was said above spores can take upwards of 3 weeks to show signs of growth. Don't panic yet OP, its likely you will see some action from these jars.
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
#23571100 - 08/23/16 10:49 PM (7 years, 5 months ago) |
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Quote:
kenetic said:
Quote:
anthiawe said: spore should go to agar not grain i believe. liquid mycelium or colonized agar should go into grain.
syringes work straight on grain jars, ime. You may not get the growth you want though, which is why I personally started agar. 6 days is nothing. Try 10-14 before you get nervous [/quote dear ken: i always used to germinate the spores in an agar type formula and then after it grew out i would inoculate the grain jars. i never used syringes like they do now. your frined is real cute i must say dear.
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17]
#23571114 - 08/23/16 10:55 PM (7 years, 5 months ago) |
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Quote:
PsilocyBen17 said:
Quote:
paracelsusgold said:
 Quote:
kenetic said: It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.
hey ken how are you feeling? if the mycelium has not started growing out after 6 days then there is something wrong and probably the spores were old or no good. one would think that there would be some contamination by then also. 
Dont mean to sound rude Parcel but this is just bad info. As was said above spores can take upwards of 3 weeks to show signs of growth. Don't panic yet OP, its likely you will see some action from these jars.
hey you are right and i realized after i wrote that that i was not clear on what i read. i always germinated the spores in a petri dish with sterilized agar formula and then after it grew out i would inoculate the substrate in jars. syringes were not available then and i never used one. so i apologize for that mistake. stay in touch ron k.
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23571126 - 08/23/16 11:00 PM (7 years, 5 months ago) |
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Quote:
Aos said: i just bought premade agar petri dishes, im tired of syringes, agar is so much better. should i drop a wedge in those jars anyways? or just dump them out, the wedge will be a different "strain"
hey what is a premade petri dish may i ask? i used to use glass ones. 
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23571133 - 08/23/16 11:03 PM (7 years, 5 months ago) |
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oh, you meant 6 days for leap off form the agar to the grains! It's all making sense now.
I was a bit disappointed myself I was thinking 'No! Ron, what do you mean?? ' Glad that got cleared up!
By the way, he means he's ordered petri dishes aleady prepared with agar and sealed. They are usually around $10+ for 4 or so. Very expensive if you plan on actually doing agar work!
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23571140 - 08/23/16 11:04 PM (7 years, 5 months ago) |
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Ron they are just already made agar in petri dishes ready to go I don't know if I would trust them but petris are expensive alot of us use pasty whytes pasty plates they are just glad ware Tupperware
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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: 6 days since inoculation no sign of colonization [Re: Boogieman47]
#23571194 - 08/23/16 11:22 PM (7 years, 5 months ago) |
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6 days is nothing. Ms to grain takes me a bit over a week and upwards to a Lil over 2 weeks at times.
--------------------
Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23571912 - 08/24/16 05:44 AM (7 years, 5 months ago) |
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Whoops
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization/ thanks [Re: dankington]
#23571996 - 08/24/16 06:37 AM (7 years, 5 months ago) |
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Quote:
dankington said: oh, you meant 6 days for leap off form the agar to the grains! It's all making sense now.
I was a bit disappointed myself I was thinking 'No! Ron, what do you mean?? ' Glad that got cleared up!
By the way, he means he's ordered petri dishes aleady prepared with agar and sealed. They are usually around $10+ for 4 or so. Very expensive if you plan on actually doing agar work!
hey dank thank you for understanding. it shows i am getting integrated into this site. you must understand that i have not done this type of work since the mid 1980s and before. as i said there were no syringes or swabs available then only spore prints from a few suppliers: peele, mckenna, few others., you used to get the amazonian from lux natura while peele had quite a few cubensis from all around. i soaked the spore print in sterilized solution and then used disposable syringes to suck up a bit of solution and inject into petri dishes that were glass with relevant agar formulas and then i was able to isolate out a strong strain better. i believe regarding my advice to that fellow that it was based on the idea that he had transferred the agar into the jar already but i see that he instead injected the syringe directly into the jars, something apparently you can do with some success? i would think intuitively that the spores might not be able to germinate on wet grain that well if at all, but again i have no eperience with these newer procedures. please stay in touch. ron kay 
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization/apologize for bad advice [Re: Aos]
#23572013 - 08/24/16 06:49 AM (7 years, 5 months ago) |
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Quote:
Aos said: Hello Shroomery friends. I have a question regarding my rye jars.
i have a few pint jars, and a few quart jars with rye. today i slighty mixed it up to see if there was any mycelium but there wasnt any. do you think it was bad spores? i used MS injection. about 1-3 CC in each jar. Any help would be appreciated.
dear friend please forgive me for the bad advice i gave you. i just reread your post and i can see now that i did not read it carefully enough. in my defense it was late and i am 70 . i really never used a syringe and i would think that you would do better if you make a proper solution with agar and sterilize it and then after it cools down inject the spore solution into the petri dish within a glovebox or sab of some type and i used to use an ionizer also. however as i said i have no experience with syringes or swabs. good luck. ronkay
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization/ thanks [Re: paracelsusgold]
#23572032 - 08/24/16 07:09 AM (7 years, 5 months ago) |
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Quote:
paracelsusgold said:
Quote:
dankington said: oh, you meant 6 days for leap off form the agar to the grains! It's all making sense now.
I was a bit disappointed myself I was thinking 'No! Ron, what do you mean?? ' Glad that got cleared up!
By the way, he means he's ordered petri dishes aleady prepared with agar and sealed. They are usually around $10+ for 4 or so. Very expensive if you plan on actually doing agar work!
hey dank thank you for understanding. it shows i am getting integrated into this site. you must understand that i have not done this type of work since the mid 1980s and before. as i said there were no syringes or swabs available then only spore prints from a few suppliers: peele, mckenna, few others., you used to get the amazonian from lux natura while peele had quite a few cubensis from all around. i soaked the spore print in sterilized solution and then used disposable syringes to suck up a bit of solution and inject into petri dishes that were glass with relevant agar formulas and then i was able to isolate out a strong strain better. i believe regarding my advice to that fellow that it was based on the idea that he had transferred the agar into the jar already but i see that he instead injected the syringe directly into the jars, something apparently you can do with some success? i would think intuitively that the spores might not be able to germinate on wet grain that well if at all, but again i have no eperience with these newer procedures. please stay in touch. ron kay 
also i would think that everything would work much better if one used glass petri dishes and made up their own formulas. i myself had a notebook for my lab work and i used many different formulas and i had a wide selection of chemicals also as pure as possible and i would mix up solutions with agar and experiment alot. i remember i used potato water quite a bit. there is one book i have that has some good formulas. anyhow i am sorry that i gave that fellow bad advice and i am going to keep my advice to myself from now on. peace and light brother
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: 6 days since inoculation no sign of colonization/ thanks [Re: paracelsusgold]
#23572053 - 08/24/16 07:19 AM (7 years, 5 months ago) |
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do you have any of those old spores lying around? that would be some amazing stuff to work with if they were still viable.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: 6 days since inoculation no sign of colonization/ thanks [Re: blindingleaf]
#23572190 - 08/24/16 08:38 AM (7 years, 5 months ago) |
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Don't be shy about giving advice, mr. Ron! There was just a miscommunication. We all see you're very knowledgeable, mr. Ron k. We like hearing from you as well.
And yes, bl! It would be amazing to have mckenna's syzygy, or pollock's tampanensis! I bet they had great specimens, though germination now would be extremely difficult
Edited by dankington (08/24/16 08:52 AM)
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization/ thanks...again [Re: dankington]
#23572524 - 08/24/16 11:02 AM (7 years, 5 months ago) |
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Quote:
dankington said: Don't be shy about giving advice, mr. Ron! There was just a miscommunication. We all see you're very knowledgeable, mr. Ron k. We like hearing from you as well.
And yes, bl! It would be amazing to have mckenna's syzygy, or pollock's tampanensis! I bet they had great specimens, though germination now would be extremely difficult
hey dank how are you. i sure do appreciate the comments however i am not sure they are deserved by me. i was working on the tampensis when i was busted. peele knew pollack and i had a print from him i think. according to peele there was only one tampensis that was found then in the wild by dr. pollack. near tampa florida maybe? i had a cigar box of prints from around the globe but cops took them all along with alot of other stuff like $. i used to order prints as well from dr. pollack in san antonio. the anazonians from terrence mckenna were nice. i cannot order any prints from these supply companies because i live in georgia. you sound like you are from the south too? i also used to use gill tissue from mushrooms for a culture. i had a couple of these speedclaves that i bought from a medical supply place going out of business that were used and older models that worked real good for petri dishes without having to use the pc. stay in touch. ronkay    
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: The Mycologist]
#23572546 - 08/24/16 11:10 AM (7 years, 5 months ago) |
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Quote:
The Mycologist said: I have inoculated rye berry with a syringe and have had full colonization of mycelium. Its not preferred but it should work.
hey mycologist how are you? may i please ask how long it took and if you got any contamination and what type of an environment you had the jars in regarding temp. humidity light etc.. what is in those syringes also? thanks alot. ronkay  
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization/ thanks [Re: Boogieman47]
#23572561 - 08/24/16 11:17 AM (7 years, 5 months ago) |
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Quote:
noob47 said: Ron they are just already made agar in petri dishes ready to go I don't know if I would trust them but petris are expensive alot of us use pasty whytes pasty plates they are just glad ware Tupperware
hey noob thank you for this info. may i please ask you why you do not make up your own cultures as it is not that hard? then everything is fresh and clean and personalized so to speak? stay in touch ron k. 
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23572570 - 08/24/16 11:20 AM (7 years, 5 months ago) |
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Ah, for saying mr. Ron, no doubt. I'm technically in the south, but right by the border. However i did live for some time in Louisiana.
But yes, i try and be respectful, as i don't know you personally.
I am well, thanks for asking. I how you are feeling well too. Bless up
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oontribe


Registered: 01/14/15
Posts: 3,570
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Re: 6 days since inoculation no sign of colonization/ thanks [Re: dankington]
#23572591 - 08/24/16 11:29 AM (7 years, 5 months ago) |
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I dont need to grow alot, only for personal use, so i am trying to keep things simple and cheap.
Thats being said i always use MS with grains (millet) and spawn to CVG, and they germinate within 3-4 days (maybe because I live in dry hot climat) and never had any contams issues as long as the prints were clean to start with.
But of course agar is the safest route and it also opens a lot of opportunities to make a lot of stuff like cleaning, cloning...etc...
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization/ more advice???? [Re: paracelsusgold]
#23572624 - 08/24/16 11:51 AM (7 years, 5 months ago) |
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Quote:
paracelsusgold said:
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Aos said: i just bought premade agar petri dishes, im tired of syringes, agar is so much better. should i drop a wedge in those jars anyways? or just dump them out, the wedge will be a different "strain"
hey what is a premade petri dish may i ask? i used to use glass ones. 
hey i am sort of afraid to tell you anything more but i would think that you should temporarily refrigerate the premade agar you got and make up another batch of jars and inoculate them with the new stuff and put other jars into a clean environment with the proper temperature etc and wait and see.  
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17]
#23572647 - 08/24/16 12:04 PM (7 years, 5 months ago) |
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PsilocyBen17 said: Spores are nitoriously filthy as they can't be harvested under sterile conditions. Like the others have said, avoid using MS to grain directly. Put it on agar first to increase your chances of getting clean spawn.
MS to grain can work, but its bad technique with a low success rate. 7 days isn't very long to wait for spore germination....you can see growth anywhere from 1-3 weeks depending on how hydrated the spores are.
Loving the lid with the Whatman filter. If you decide to stick with the hobby you might want to invest in proper SFDs. Poly is a good budget option but it needs to be stuffed super tight to avoid contamination.
Welcome to the Shroomery brother. Hope you stick around.
dear benny how are you? i would please like to ask you what is a sfd and also what does ms mean? i used to use a nice special glass syringe with special filters to take the contaminants off the spores from peele. i still have it and a few filters i believe. it worked good. also what is that filter all about. stay in touch. ronkay  
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blackout


Registered: 07/16/00
Posts: 5,266
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23572684 - 08/24/16 12:19 PM (7 years, 5 months ago) |
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Aos said: Hello Shroomery friends. I have a question regarding my rye jars.
i have a few pint jars, and a few quart jars with rye. today i slighty mixed it up to see if there was any mycelium but there wasnt any. do you think it was bad spores? i used MS injection. about 1-3 CC in each jar.
As said 3cc is too much and 6 days is not a lot. Where did you inject it into the jar? some will do it down the side of the jar, I mark my jars with a marker if I do this. I would not shake so the spores do not get too spread out and I know what spot to start looking for growth in. Otherwise I would drip spores in the centre and again not shake so I know where to look. I also use agar these days as others have recommended.
Not only is 3cc using up what I presume are commercial syringes but you have a greater chance of contaminants. if there are 10 contams in the syringe its very likely one went in with 3cc.
There could have been very slight growth going on but now you shook them up it might take even longer before you see it as it will now be spread out and recover slowly in many different places.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
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Re: 6 days since inoculation no sign of colonization [Re: blackout]
#23572961 - 08/24/16 02:12 PM (7 years, 5 months ago) |
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Nice answer
I'd also like to add you don't want the spores to spread out too much when you shoot them up because that will make it harder for the spore's mono myc to mate with each other. That fast rhizo growth you see is the result of two mushroom spores mating.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: 6 days since inoculation no sign of colonization/ thanks [Re: paracelsusgold]
#23573712 - 08/24/16 06:01 PM (7 years, 5 months ago) |
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paracelsusgold said:
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noob47 said: Ron they are just already made agar in petri dishes ready to go I don't know if I would trust them but petris are expensive alot of us use pasty whytes pasty plates they are just glad ware Tupperware
hey noob thank you for this info. may i please ask you why you do not make up your own cultures as it is not that hard? then everything is fresh and clean and personalized so to speak? stay in touch ron k.  
I do Ron I'm using malt extract agar and potato dextrose agar and have 13 varieties bit only 6 actively going right now .... I haven't been doing this long but I do get better with each grow man you can check my gallery under my name it you can see my pics take it easy Ron
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization/ [Re: Boogieman47]
#23574849 - 08/25/16 01:38 AM (7 years, 5 months ago) |
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noob47 said:
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paracelsusgold said:
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noob47 said: Ron they are just already made agar in petri dishes ready to go I don't know if I would trust them but petris are expensive alot of us use pasty whytes pasty plates they are just glad ware Tupperware
hey noob thank you for this info. may i please ask you why you do not make up your own cultures as it is not that hard? then everything is fresh and clean and personalized so to speak? stay in touch ron k.  
I do Ron I'm using malt extract agar and potato dextrose agar and have 13 varieties bit only 6 actively going right now .... I haven't been doing this long but I do get better with each grow man you can check my gallery under my name it you can see my pics take it easy Ron [/quote noob it sounds like you know what you are doing. i used to use malt extract too. i had alot of different chemicals reagent grade. i can give you some formulas if you would like? i had two boxes of those chemicals i dragged around with me when i moved from new york to oklahoma and then to arkansas and finally in about 2003 or so i dumped them and i had them from the early 80's. i can collect them again from a scientific companyt easily enough which i plan on doing. i used these speedclaves that were what doctors used to use in their offices and i bought them used (2) and i used one all the time for my mycological work as it was much easier than the pressure cooker even with a special tray that i have for petri dishes in pressure cookers ( i see they sell for $100 today). also i used to use a special glass syringe i got from peele of the fmrc that had a special attachment for filters that would "clean" the spores and that seemed to work good. i still have it. anyhoiw stay in touch. ronkay   
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Tuhdoww
Sub Slapper


Registered: 08/23/16
Posts: 300
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23574866 - 08/25/16 01:56 AM (7 years, 5 months ago) |
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paracelsusgold said:
one would think that there would be some contamination by then also. 
Shouldn't be a contamination issue after just 6 days. With this logic how would anyone get a fully colonized jar before 6 days. If you did your shit right there is no way it'll contaminate within 6 days
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