|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
|
Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23572570 - 08/24/16 11:20 AM (7 years, 5 months ago) |
|
|
Ah, for saying mr. Ron, no doubt. I'm technically in the south, but right by the border. However i did live for some time in Louisiana.
But yes, i try and be respectful, as i don't know you personally.
I am well, thanks for asking. I how you are feeling well too. Bless up
|
oontribe


Registered: 01/14/15
Posts: 3,570
|
Re: 6 days since inoculation no sign of colonization/ thanks [Re: dankington]
#23572591 - 08/24/16 11:29 AM (7 years, 5 months ago) |
|
|
I dont need to grow alot, only for personal use, so i am trying to keep things simple and cheap.
Thats being said i always use MS with grains (millet) and spawn to CVG, and they germinate within 3-4 days (maybe because I live in dry hot climat) and never had any contams issues as long as the prints were clean to start with.
But of course agar is the safest route and it also opens a lot of opportunities to make a lot of stuff like cleaning, cloning...etc...
|
paracelsusgold

Registered: 08/15/16
Posts: 173
|
Re: 6 days since inoculation no sign of colonization/ more advice???? [Re: paracelsusgold]
#23572624 - 08/24/16 11:51 AM (7 years, 5 months ago) |
|
|
Quote:
paracelsusgold said:
Quote:
Aos said: i just bought premade agar petri dishes, im tired of syringes, agar is so much better. should i drop a wedge in those jars anyways? or just dump them out, the wedge will be a different "strain"
hey what is a premade petri dish may i ask? i used to use glass ones. 
hey i am sort of afraid to tell you anything more but i would think that you should temporarily refrigerate the premade agar you got and make up another batch of jars and inoculate them with the new stuff and put other jars into a clean environment with the proper temperature etc and wait and see.  
|
paracelsusgold

Registered: 08/15/16
Posts: 173
|
Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17]
#23572647 - 08/24/16 12:04 PM (7 years, 5 months ago) |
|
|
Quote:
PsilocyBen17 said: Spores are nitoriously filthy as they can't be harvested under sterile conditions. Like the others have said, avoid using MS to grain directly. Put it on agar first to increase your chances of getting clean spawn.
MS to grain can work, but its bad technique with a low success rate. 7 days isn't very long to wait for spore germination....you can see growth anywhere from 1-3 weeks depending on how hydrated the spores are.
Loving the lid with the Whatman filter. If you decide to stick with the hobby you might want to invest in proper SFDs. Poly is a good budget option but it needs to be stuffed super tight to avoid contamination.
Welcome to the Shroomery brother. Hope you stick around.
dear benny how are you? i would please like to ask you what is a sfd and also what does ms mean? i used to use a nice special glass syringe with special filters to take the contaminants off the spores from peele. i still have it and a few filters i believe. it worked good. also what is that filter all about. stay in touch. ronkay  
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
|
Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23572684 - 08/24/16 12:19 PM (7 years, 5 months ago) |
|
|
Quote:
Aos said: Hello Shroomery friends. I have a question regarding my rye jars.
i have a few pint jars, and a few quart jars with rye. today i slighty mixed it up to see if there was any mycelium but there wasnt any. do you think it was bad spores? i used MS injection. about 1-3 CC in each jar.
As said 3cc is too much and 6 days is not a lot. Where did you inject it into the jar? some will do it down the side of the jar, I mark my jars with a marker if I do this. I would not shake so the spores do not get too spread out and I know what spot to start looking for growth in. Otherwise I would drip spores in the centre and again not shake so I know where to look. I also use agar these days as others have recommended.
Not only is 3cc using up what I presume are commercial syringes but you have a greater chance of contaminants. if there are 10 contams in the syringe its very likely one went in with 3cc.
There could have been very slight growth going on but now you shook them up it might take even longer before you see it as it will now be spread out and recover slowly in many different places.
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: 6 days since inoculation no sign of colonization [Re: blackout]
#23572961 - 08/24/16 02:12 PM (7 years, 5 months ago) |
|
|
Nice answer
I'd also like to add you don't want the spores to spread out too much when you shoot them up because that will make it harder for the spore's mono myc to mate with each other. That fast rhizo growth you see is the result of two mushroom spores mating.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
Re: 6 days since inoculation no sign of colonization/ thanks [Re: paracelsusgold]
#23573712 - 08/24/16 06:01 PM (7 years, 5 months ago) |
|
|
Quote:
paracelsusgold said:
Quote:
noob47 said: Ron they are just already made agar in petri dishes ready to go I don't know if I would trust them but petris are expensive alot of us use pasty whytes pasty plates they are just glad ware Tupperware
hey noob thank you for this info. may i please ask you why you do not make up your own cultures as it is not that hard? then everything is fresh and clean and personalized so to speak? stay in touch ron k.  
I do Ron I'm using malt extract agar and potato dextrose agar and have 13 varieties bit only 6 actively going right now .... I haven't been doing this long but I do get better with each grow man you can check my gallery under my name it you can see my pics take it easy Ron
|
paracelsusgold

Registered: 08/15/16
Posts: 173
|
Re: 6 days since inoculation no sign of colonization/ [Re: Boogieman47]
#23574849 - 08/25/16 01:38 AM (7 years, 5 months ago) |
|
|
Quote:
noob47 said:
Quote:
paracelsusgold said:
Quote:
noob47 said: Ron they are just already made agar in petri dishes ready to go I don't know if I would trust them but petris are expensive alot of us use pasty whytes pasty plates they are just glad ware Tupperware
hey noob thank you for this info. may i please ask you why you do not make up your own cultures as it is not that hard? then everything is fresh and clean and personalized so to speak? stay in touch ron k.  
I do Ron I'm using malt extract agar and potato dextrose agar and have 13 varieties bit only 6 actively going right now .... I haven't been doing this long but I do get better with each grow man you can check my gallery under my name it you can see my pics take it easy Ron [/quote noob it sounds like you know what you are doing. i used to use malt extract too. i had alot of different chemicals reagent grade. i can give you some formulas if you would like? i had two boxes of those chemicals i dragged around with me when i moved from new york to oklahoma and then to arkansas and finally in about 2003 or so i dumped them and i had them from the early 80's. i can collect them again from a scientific companyt easily enough which i plan on doing. i used these speedclaves that were what doctors used to use in their offices and i bought them used (2) and i used one all the time for my mycological work as it was much easier than the pressure cooker even with a special tray that i have for petri dishes in pressure cookers ( i see they sell for $100 today). also i used to use a special glass syringe i got from peele of the fmrc that had a special attachment for filters that would "clean" the spores and that seemed to work good. i still have it. anyhoiw stay in touch. ronkay   
|
Tuhdoww
Sub Slapper


Registered: 08/23/16
Posts: 300
|
Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23574866 - 08/25/16 01:56 AM (7 years, 5 months ago) |
|
|
Quote:
paracelsusgold said:
one would think that there would be some contamination by then also. 
Shouldn't be a contamination issue after just 6 days. With this logic how would anyone get a fully colonized jar before 6 days. If you did your shit right there is no way it'll contaminate within 6 days
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 2,548 topic views. 39 members, 375 guests and 147 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|