Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Cultivation Supplies   Original Sensible Seeds High THC Strains   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineAos
011101110111010001100110
Male User Gallery


Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
6 days since inoculation no sign of colonization
    #23569541 - 08/23/16 03:50 PM (7 years, 5 months ago)

Hello Shroomery friends. I have a question regarding my rye jars.

i have a few pint jars, and a few quart jars with rye. today i slighty mixed it up to see if there was any mycelium but there wasnt any. do you think it was bad spores? i used MS injection. about 1-3 CC in each jar. Any help would be appreciated.



Extras: Filter Print Post Top
Offlinespore-ty
Male


Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
Re: 6 days since inoculation no sign of colonization [Re: Aos]
    #23569554 - 08/23/16 03:53 PM (7 years, 5 months ago)

Is that the camera flash or glare or something in those jars?


I see mycelium..


Extras: Filter Print Post Top
OfflineAos
011101110111010001100110
Male User Gallery


Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
    #23569558 - 08/23/16 03:54 PM (7 years, 5 months ago)

yes camera flash. not mycelium.


Edited by Aos (08/23/16 03:55 PM)


Extras: Filter Print Post Top
Offlinespore-ty
Male


Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
Re: 6 days since inoculation no sign of colonization [Re: Aos]
    #23569562 - 08/23/16 03:55 PM (7 years, 5 months ago)

Lol dude are you sure there's not 'white stuff' in your jars?


Extras: Filter Print Post Top
OfflineAos
011101110111010001100110
Male User Gallery


Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
    #23569589 - 08/23/16 04:05 PM (7 years, 5 months ago)

i am absolutely positive there is no mycelium, i rolled them in my hands for like 5 minutes closely observing. no mycelium. they white you see in the photo is from the camera flash.


Extras: Filter Print Post Top
Offlineanthiawe
friendly stranger
 User Gallery

Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
Re: 6 days since inoculation no sign of colonization [Re: Aos]
    #23569598 - 08/23/16 04:10 PM (7 years, 5 months ago)

spore should go to agar not grain i believe. liquid mycelium or colonized agar should go into grain.


--------------------
TEK compendium


Extras: Filter Print Post Top
OfflineAos
011101110111010001100110
Male User Gallery


Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
Re: 6 days since inoculation no sign of colonization [Re: anthiawe]
    #23569606 - 08/23/16 04:12 PM (7 years, 5 months ago)

never heard that one before.


Extras: Filter Print Post Top
Offlinespore-ty
Male


Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
Re: 6 days since inoculation no sign of colonization [Re: Aos]
    #23569612 - 08/23/16 04:16 PM (7 years, 5 months ago)

Spores to agar then agar to mother jar (s) or lc for expansion and you know it's clean so it's all in your aseptic technique


Extras: Filter Print Post Top
OfflineAos
011101110111010001100110
Male User Gallery


Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
    #23569625 - 08/23/16 04:22 PM (7 years, 5 months ago)

i just bought premade agar petri dishes, im tired of syringes, agar is so much better. should i drop a wedge in those jars anyways? or just dump them out, the wedge will be a different "strain"


Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 12 hours
Re: 6 days since inoculation no sign of colonization [Re: Aos]
    #23569637 - 08/23/16 04:26 PM (7 years, 5 months ago)

I have inoculated rye berry with a syringe and have had full colonization of mycelium. Its not preferred but it should work.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


Extras: Filter Print Post Top
Invisibledankington
The Stranger
Male User Gallery


Folding@home Statistics
Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: 6 days since inoculation no sign of colonization [Re: Aos]
    #23569644 - 08/23/16 04:29 PM (7 years, 5 months ago)

premade agar dishes? You're going to really want to use more than just a couple of dishes. I rotate through ~20-30 weekly, and I'm a very minor grower. If I were serious, I'd be going through hundreds a month! Being able to toss something readily is really nice. You don't want to end up trying to save hopeless specimens. You also want to be able to take several transfers.

Also spores to grain
:justno:

especially not 1-3cc, that's way too much! Next time, if you must, just try expel just a drop right on the top of the grains.

Also, 6 days is really not enough time to get worried.


Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 3 months
Re: 6 days since inoculation no sign of colonization [Re: anthiawe]
    #23569659 - 08/23/16 04:38 PM (7 years, 5 months ago)

Quote:

anthiawe said:
spore should go to agar not grain i believe. liquid mycelium or colonized agar should go into grain.




syringes work straight on grain jars, ime.  You may not get the growth you want though, which is why I personally started agar.
6 days is nothing.  Try 10-14 before you get nervous


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Extras: Filter Print Post Top
Invisibleparacelsusgold

Registered: 08/15/16
Posts: 173
Re: 6 days since inoculation no sign of colonization [Re: Aos]
    #23569707 - 08/23/16 04:57 PM (7 years, 5 months ago)

how long has been since you inoculated them?


Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 3 months
Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
    #23569721 - 08/23/16 05:02 PM (7 years, 5 months ago)

It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
    #23569722 - 08/23/16 05:03 PM (7 years, 5 months ago)

Quote:

paracelsusgold said:
how long has been since you inoculated them?




Good question
Hmmm:jah:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Extras: Filter Print Post Top
OfflinePsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
    #23569727 - 08/23/16 05:04 PM (7 years, 5 months ago)

Spores are nitoriously filthy as they can't be harvested under sterile conditions. Like the others have said, avoid using MS to grain directly. Put it on agar first to increase your chances of getting clean spawn.

MS to grain can work, but its bad technique with a low success rate. 7 days isn't very long to wait for spore germination....you can see growth anywhere from 1-3 weeks depending on how hydrated the spores are.

Loving the lid with the Whatman filter. If you decide to stick with the hobby you might want to invest in proper SFDs. Poly is a good budget option but it needs to be stuffed super tight to avoid contamination.

Welcome to the Shroomery brother. Hope you stick around. :thumbup:


--------------------
PsilocyBen's Trade List!


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 9 days
Trusted Cultivator
Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17] * 1
    #23569758 - 08/23/16 05:11 PM (7 years, 5 months ago)

6 days means two things....you didn't get any contams quickly and you lack patience lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineAos
011101110111010001100110
Male User Gallery


Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17]
    #23570002 - 08/23/16 06:14 PM (7 years, 5 months ago)

:smile:


Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: 6 days since inoculation no sign of colonization [Re: cronicr]
    #23570024 - 08/23/16 06:20 PM (7 years, 5 months ago)

Quote:

cronicr said:
6 days means two things....you didn't get any contams quickly and you lack patience lol





This. Ive had spores take 3 weeks to germinate before. Its rare, but does happen.


Extras: Filter Print Post Top
Invisibleparacelsusgold

Registered: 08/15/16
Posts: 173
Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
    #23570815 - 08/23/16 09:43 PM (7 years, 5 months ago)

:breakthrough:
Quote:

kenetic said:
It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.


hey ken how are you feeling? if the mycelium has not started growing out after 6 days then there is something wrong and probably the spores were old or no good. one would think that there would be some contamination by then also. :breakthrough:


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: North Spore Cultivation Supplies   Original Sensible Seeds High THC Strains   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Inoculation in a few days.... triplethreat 1,157 13 10/11/03 11:28 PM
by Hertyar
* Colonized Manure in Terrarium? teen 862 6 02/19/04 07:39 PM
by teen
* inoculation question Snoop 1,272 4 07/26/02 05:18 AM
by FanaTEK
* inoculating tupperwares destello 900 5 09/29/03 12:53 AM
by BrainFarmer
* WBS 1qt Jars - 7 days still no signs of colonization. TwistedTR 3,437 15 05/30/03 03:59 PM
by HaveABeer420
* inoculation, HELP?? dimitri211 732 5 04/30/02 09:51 AM
by Seuss
* First signs of colonization Partysan 5,142 9 04/12/02 02:56 AM
by Partysan
* colonize damn you!
( 1 2 all )
celestialtripper 1,759 21 02/05/03 05:38 PM
by celestialtripper

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,548 topic views. 22 members, 325 guests and 89 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.006 seconds on 14 queries.