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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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6 days since inoculation no sign of colonization
#23569541 - 08/23/16 03:50 PM (7 years, 5 months ago) |
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Hello Shroomery friends. I have a question regarding my rye jars.
i have a few pint jars, and a few quart jars with rye. today i slighty mixed it up to see if there was any mycelium but there wasnt any. do you think it was bad spores? i used MS injection. about 1-3 CC in each jar. Any help would be appreciated.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569554 - 08/23/16 03:53 PM (7 years, 5 months ago) |
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Is that the camera flash or glare or something in those jars?
I see mycelium..
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
#23569558 - 08/23/16 03:54 PM (7 years, 5 months ago) |
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yes camera flash. not mycelium.
Edited by Aos (08/23/16 03:55 PM)
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569562 - 08/23/16 03:55 PM (7 years, 5 months ago) |
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Lol dude are you sure there's not 'white stuff' in your jars?
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
#23569589 - 08/23/16 04:05 PM (7 years, 5 months ago) |
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i am absolutely positive there is no mycelium, i rolled them in my hands for like 5 minutes closely observing. no mycelium. they white you see in the photo is from the camera flash.
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anthiawe
friendly stranger


Registered: 05/18/16
Posts: 652
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569598 - 08/23/16 04:10 PM (7 years, 5 months ago) |
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spore should go to agar not grain i believe. liquid mycelium or colonized agar should go into grain.
-------------------- TEK compendium
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: anthiawe]
#23569606 - 08/23/16 04:12 PM (7 years, 5 months ago) |
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never heard that one before.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569612 - 08/23/16 04:16 PM (7 years, 5 months ago) |
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Spores to agar then agar to mother jar (s) or lc for expansion and you know it's clean so it's all in your aseptic technique
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: spore-ty]
#23569625 - 08/23/16 04:22 PM (7 years, 5 months ago) |
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i just bought premade agar petri dishes, im tired of syringes, agar is so much better. should i drop a wedge in those jars anyways? or just dump them out, the wedge will be a different "strain"
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569637 - 08/23/16 04:26 PM (7 years, 5 months ago) |
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I have inoculated rye berry with a syringe and have had full colonization of mycelium. Its not preferred but it should work.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569644 - 08/23/16 04:29 PM (7 years, 5 months ago) |
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premade agar dishes? You're going to really want to use more than just a couple of dishes. I rotate through ~20-30 weekly, and I'm a very minor grower. If I were serious, I'd be going through hundreds a month! Being able to toss something readily is really nice. You don't want to end up trying to save hopeless specimens. You also want to be able to take several transfers.
Also spores to grain

especially not 1-3cc, that's way too much! Next time, if you must, just try expel just a drop right on the top of the grains.
Also, 6 days is really not enough time to get worried.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
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Re: 6 days since inoculation no sign of colonization [Re: anthiawe]
#23569659 - 08/23/16 04:38 PM (7 years, 5 months ago) |
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Quote:
anthiawe said: spore should go to agar not grain i believe. liquid mycelium or colonized agar should go into grain.
syringes work straight on grain jars, ime. You may not get the growth you want though, which is why I personally started agar. 6 days is nothing. Try 10-14 before you get nervous
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: Aos]
#23569707 - 08/23/16 04:57 PM (7 years, 5 months ago) |
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how long has been since you inoculated them?
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
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Re: 6 days since inoculation no sign of colonization [Re: paracelsusgold]
#23569721 - 08/23/16 05:02 PM (7 years, 5 months ago) |
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It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
#23569722 - 08/23/16 05:03 PM (7 years, 5 months ago) |
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Quote:
paracelsusgold said: how long has been since you inoculated them?
Good question Hmmm
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
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Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
#23569727 - 08/23/16 05:04 PM (7 years, 5 months ago) |
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Spores are nitoriously filthy as they can't be harvested under sterile conditions. Like the others have said, avoid using MS to grain directly. Put it on agar first to increase your chances of getting clean spawn.
MS to grain can work, but its bad technique with a low success rate. 7 days isn't very long to wait for spore germination....you can see growth anywhere from 1-3 weeks depending on how hydrated the spores are.
Loving the lid with the Whatman filter. If you decide to stick with the hobby you might want to invest in proper SFDs. Poly is a good budget option but it needs to be stuffed super tight to avoid contamination.
Welcome to the Shroomery brother. Hope you stick around.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17] 1
#23569758 - 08/23/16 05:11 PM (7 years, 5 months ago) |
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6 days means two things....you didn't get any contams quickly and you lack patience lol
--------------------
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Aos
011101110111010001100110



Registered: 03/30/16
Posts: 33
Last seen: 6 years, 8 months
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Re: 6 days since inoculation no sign of colonization [Re: PsilocyBen17]
#23570002 - 08/23/16 06:14 PM (7 years, 5 months ago) |
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
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Re: 6 days since inoculation no sign of colonization [Re: cronicr]
#23570024 - 08/23/16 06:20 PM (7 years, 5 months ago) |
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Quote:
cronicr said: 6 days means two things....you didn't get any contams quickly and you lack patience lol
This. Ive had spores take 3 weeks to germinate before. Its rare, but does happen.
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: 6 days since inoculation no sign of colonization [Re: Kenetic]
#23570815 - 08/23/16 09:43 PM (7 years, 5 months ago) |
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 Quote:
kenetic said: It's been like 6 days since he inoculated and he still hasn't seen any signs of colonization.
hey ken how are you feeling? if the mycelium has not started growing out after 6 days then there is something wrong and probably the spores were old or no good. one would think that there would be some contamination by then also.
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