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OfflineKenetic
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My first germination on agar
    #23569428 - 08/23/16 03:05 PM (7 years, 5 months ago)

So....I guess I did better than I thought. 

Last friday or so I poured agar for my first time and I overfilled sections, got it on the sides of a couple dishes, and all around had a very sloppy agar pouring session.  I poured 25 3-section plates and expected 5 maybe to be contam free, but it's tuesday now and none of my dishes have contaminated.  I got lucky!!!  Well, except with one, and I'm about to show you.  b=

I nocc'ed 'em up with a cubensis print I received lately and I noticed some clear mycelium growing on sunday, but now it has turned white.  Here we go:









As you can see I have a nasty contam on one section of the plates, but I'm not worried about it because it came from the print and not my sterile tech (which sucked but apparently was good enough.

Let me know what you think.  Am I off to a good start?

Edit:  fixed a typo


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Todo Cambia
   

               
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Edited by Kenetic (08/23/16 04:44 PM)


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InvisibleTuhdoww
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Registered: 08/23/16
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Re: My first germination on agar [Re: Kenetic]
    #23569434 - 08/23/16 03:07 PM (7 years, 5 months ago)

That's not luck, that's skill!! :cool:


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Offlinemrmazdarx9
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Re: My first germination on agar [Re: Tuhdoww]
    #23569453 - 08/23/16 03:14 PM (7 years, 5 months ago)

Quote:

Tuhdoww said:
That's not luck, that's skill!! :cool:



Yep way better than my first second and third try my forth was better always improving


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OfflineKenetic
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Re: My first germination on agar [Re: mrmazdarx9]
    #23569498 - 08/23/16 03:34 PM (7 years, 5 months ago)

It's fun as hell I'm kind of pissed I went so long without it.  Glad I hit up so many dishes, now I can really pic the best growth and go from there.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleComebackKid
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Re: My first germination on agar [Re: Kenetic]
    #23569802 - 08/23/16 05:24 PM (7 years, 5 months ago)

Fuck yeah! :congrats:

Good to know that the clear strands arent a bad thing.


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OfflineKenetic
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Re: My first germination on agar [Re: ComebackKid]
    #23569814 - 08/23/16 05:27 PM (7 years, 5 months ago)

I think it might just be the monokaryotic myc


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineKenetic
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Re: My first germination on agar [Re: Kenetic]
    #23572168 - 08/24/16 08:22 AM (7 years, 5 months ago)

Can I talk a mod into moving this thread to grow logs?  I'm going to keep updating it


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflinePsilocyBen17
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Re: My first germination on agar [Re: Kenetic]
    #23572343 - 08/24/16 09:56 AM (7 years, 5 months ago)

ohhh i love those Milwaukee fine tipped markers.


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OfflineWeavieWonder
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Re: My first germination on agar [Re: PsilocyBen17]
    #23573618 - 08/24/16 05:29 PM (7 years, 5 months ago)

I'm looking to get into agar work as well.  What kind of agar did you use?  Nice job on your results!  I can only hope my first attempt will be that successful.


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OfflineKenetic
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Re: My first germination on agar [Re: WeavieWonder]
    #23573713 - 08/24/16 06:01 PM (7 years, 5 months ago)

I used a premix MEA.  It's pretty easy but probably a lot cheaper to mix myself.  I'm surprised it turned out so well myself.  I'm gonna make a couple transfers tonight from the dish with the contam and transfer the other four dishes in a day or two when some rhizo growth appears.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflinePsilocyBen17
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Re: My first germination on agar [Re: Kenetic]
    #23573724 - 08/24/16 06:05 PM (7 years, 5 months ago)

Tell me about it, I bought MEA agar from FP and its costs like $40+shipping. Last week I mixed my own with $2.50 worth of agar agar and $1.50 of potato flakes,


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OfflineKenetic
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Re: My first germination on agar [Re: PsilocyBen17]
    #23573737 - 08/24/16 06:10 PM (7 years, 5 months ago)

That's awesome.  I just bought 100 more petri's too and the cost of agar is really gonna add up soon with the way I plan on using it.  Looks like I'll be hitting up the home brew store soon. 

The markers are great too.  I like them better than sharpies cause the clip is a lot stronger and it stays in my shirt pocket all day lol.  Speaking of which I really need to label these dishes.  These are the 'a' series with a number and the next transfers will be 'b' with a corresponding number.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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