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Offlinehellkid135
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Extra Jar and Spore syringes
    #23568906 - 08/23/16 11:36 AM (7 years, 5 months ago)

Hey guys, so I'm actually in the middle of growing for the first time. Used the PF-Tek, so I have 5 jars sitting in a clean drawer right now because I inoculated them about 3 days ago. However, I ordered 3 spore syringes altogether, so I only used 1 syringe so far. I have 1, 8oz jar left over and 2 spore syringes. I was wondering if there's any method of growing with only 1, 8oz jar and a spore syringe. Still kinda new, so sorry if it's a dumb question. I've heard of LC, and the process and such, but I'm not really clear on what to do after you grow mycelia in it and then suck it back into the syringe. Again, sorry for kind of a noob post. Just trying to get some knowledge here though. Thanks for the help.


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InvisibleInocuole
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Re: Extra Jar and Spore syringes [Re: hellkid135]
    #23569156 - 08/23/16 01:31 PM (7 years, 5 months ago)

You used a whole syringe on 5 jars?  One 10cc syringe should make 10 PF jars.

Don't even worry about LC, especially if you're new.  I don't even worry about LC and I've been doing this for years.  If you want to stretch out your spores dilute them into sterile water, or learn agar.  You could probably shoot a 10cc spore syringe into a pint of water and aspirate however many syringes that makes back out of it and they would all work, so long as you used good technique while doing so.  You could potentially ruin them that way though if you're not confident with the handling.  Learning agar will teach you and make you confident enough to be able to make LC or whatever else you want without significantly impacting your chances of success like jumping the gun might do.

Whatever you do, use a still air box and utilize best practices.

http://www.shroomery.org/forums/showflat.php/Number/20048771#20048771
https://www.shroomery.org/forums/showflat.php/Number/22721954


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Offlinehellkid135
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Re: Extra Jar and Spore syringes [Re: Inocuole]
    #23569806 - 08/23/16 05:25 PM (7 years, 5 months ago)

I did use a whole 10cc syringe on 5 jars :/. I don't know, not the smartest decision. Didn't think about it I guess. But thank you for the advice and taking the time to read. I'm going to read up on Agar. Seems interesting.


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OfflineKenetic
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Re: Extra Jar and Spore syringes [Re: hellkid135]
    #23569828 - 08/23/16 05:31 PM (7 years, 5 months ago)

a big reason is because you screw up your water content.

Plus your wasting spores, and less is better anyway.


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Offlinehellkid135
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Re: Extra Jar and Spore syringes [Re: Kenetic]
    #23569892 - 08/23/16 05:51 PM (7 years, 5 months ago)

Damn. That makes sense. You think that could've messed up any of the jars because of the extra water content? Either way I guess I know that now for the future.


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InvisibleBoogieman47
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Re: Extra Jar and Spore syringes [Re: hellkid135]
    #23571785 - 08/24/16 04:12 AM (7 years, 5 months ago)

You will know in a week or so if it did.. like noc said look into agar you're going to have alot of time waiting so build you a sab and get some agar going or you will be checking your jars every five seconds use the time to help yourself instead of stressing man agar is easy just mind over matter bud


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OfflineRealmLAir
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Re: Extra Jar and Spore syringes [Re: hellkid135]
    #23572525 - 08/24/16 11:02 AM (7 years, 5 months ago)

not saying i disagree with the above posters....but look up the "Karo LC Tek". basically i was able to take .5-1 ccs of a spore syringe and make over 50 ccs of usable liquid culture...everybody says LCs are a risk/crap shoot and that you have to test on agar first...but i went ahead and have successfully knocked up 6 jars with it already. NO contams

for shits and giggles i even noc'd up a jar using 7 whole ccs of LC. shot it straight into the middle of the jar and now they are exploding from the inside out. no contams. water content was not affected. its still growing thick white healthy myc

The only "contams" i had was when i shot the LC onto agar and a pink bacteria formed, a week later, strong healthy mycelium is slowly but surely taking over. No pink left. I believe spores/mycelium to be very versatile to their environments


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InvisibleInocuole
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Re: Extra Jar and Spore syringes [Re: RealmLAir]
    #23572757 - 08/24/16 12:50 PM (7 years, 5 months ago)

I don't believe anyone who says things like they shot up LC with spores and then got no contams.  Sounds like someone who doesn't know how to spot contams.

If you shoot it on agar and nasty shit grows, that means it's happening on grain too.

Nobody within their right mind will ever suggest making LC with spores.  If you need LC for some reason, you should be inoculating it with agar.

A single bacterium in a spore syringe can completely fuck a LC.  If you think getting lucky is a skill and you've mastered it then more power to you..


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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