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c10h12n2o
serial dilutor



Registered: 01/21/15
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Back in the Saddle / AA+ Pin Porn
#23560351 - 08/20/16 04:02 PM (7 years, 5 months ago) |
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hello lovely people I had taken a break from the hobby for a while and now have begun practicing (rather than just lurking and reading) again in earnest this summer.
Most of my recent work has been on agar, doing isolations on several races and species. After several series of transfers i made LIs from some of the sexiest plates and started making spawn with these, making them somewhat limited MS
Out of everything, the AA+ has shown the most rhizo growth and been the fastest, so much so that it got put in fruiting conditions more than a full week before other tubs created the same day. wheat berries spawned to hpoo, coir, verm, straw, and gypsum.
Here are some pics from today, looking like it is going to be a nice pinset, covered in hyphal knots 

covered in these:
 Get Hyphy!
I have never worked with AA+ before, and it has been a couple of years since i have been involved with practicing the hobby. Does everything look alright? any suggested intervention, or any other tips about proceeding from here? I have been REALLY lucky with my monotubs in the past but i am trying to dial in my technique before i start testing all these isolates and would appreciate any feedback
thanks everyone! 
ps: i have some really exciting spawn and plates going right now, including p. micro. (polyurethane digesting fungus), cordyceps militaris , pan tropicalis (pan cyan?), reshi, and a ridiculously fast PE6 culture and bulk substrate colonizing for lions mane, shaggy mane, blue oyster buckets, pheonix/lung oyster buckets, and many more! should be a busy summer!!
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23560480 - 08/20/16 04:36 PM (7 years, 5 months ago) |
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Looking good man!
I'm in no way the most skilled cultivator here. But I would say you're on the right track.
Looks.like you have a lot of specimens rolling. Should be a really fun time!
Oysters are my area of experience... I would love to see you post some updates when you get those into spawn.
Nice job man. Keep us updated when that tub explodes!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
#23560536 - 08/20/16 04:57 PM (7 years, 5 months ago) |
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Looks great
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c10h12n2o
serial dilutor



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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
#23560653 - 08/20/16 05:37 PM (7 years, 5 months ago) |
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thanks man!
I am having a lot of fun with it, learning lots thanks in no small part to this wonderful community. I am really excited to play around with all these isolates, and harvest/clone the MS in the meantime. I have some shaggy mane and lions mane colonizing bulk right now, my first time with both, looking great so far though! lions mane had pins in the grain jars!
i would love some feedback on the oysters too, since its my first experience with them as well, would be great to have someone experienced handy I have 1 phoenix/lung oyster bucket going right now that might be ready to fruit, im not really sure and i have 2 smaller blue oyster buckets that have been colonizing for 5 days, probably about time to check on them (though i kinda have low expectations for them despite the beautiful grainspawn, because my dumb ass spilled the spawn on concrete while it was draining on the window screen after soaking the grains a la Frank, i salvaged what seemed clean , but my expectations arent high considering this. needless to say, i got a rope instead of rubber bands to hold the screen from now on :/ what would you like pics of? i have documented most of the process. any feedback is much appreciated
thanks pasty!! you definitely deserve some of the credit cant thank you enough for everything you do around here
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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c10h12n2o
serial dilutor



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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23563066 - 08/21/16 01:38 PM (7 years, 5 months ago) |
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some pics from this morning:

there are actually way more pins and hyphal knots that can be seen in the pics, had trouble finding a spot without condensation to take pics from. This is my first experience with AA+, its so crazy how lightly colored the pins are, so cool
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23563072 - 08/21/16 01:41 PM (7 years, 5 months ago) |
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Pins!!!
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Pastywhyte
Say hello to my little friend



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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
#23563086 - 08/21/16 01:49 PM (7 years, 5 months ago) |
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Just take the lid off to snap a clear picture. Won't hurt a thing.
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morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
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Re: Back in the Saddle / AA+ Pin Porn [Re: Pastywhyte]
#23563167 - 08/21/16 02:43 PM (7 years, 5 months ago) |
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Quote:
Pastywhyte said: Just take the lid off to snap a clear picture. Won't hurt a thing.
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c10h12n2o
serial dilutor



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Re: Back in the Saddle / AA+ Pin Porn [Re: Pastywhyte]
#23563244 - 08/21/16 03:23 PM (7 years, 5 months ago) |
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thanks for the tip my pasty brother, here are some better shots with the lid off still dont stick out as much in photos since its leucistic(sp?) but im stoked about all the hyphal knots and tiny pins still starting this is my first tub in a while, so if theres anything i should do to make the most of it i am all ears



I have a BUNCH of stuff in the works, should be a very busy summer cant wait to start testing all these isolates i have been working on, and the cordyceps, and the reishi, lions mane, shaggy mane, lots of oysters, and the p. micro (plastic digesting) really want to dial in my techniques and sharpen up before i start testing isolates
thanks again everyone!
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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c10h12n2o
serial dilutor



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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23566108 - 08/22/16 02:08 PM (7 years, 5 months ago) |
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here are some shots from this morning 

coming along beautifully its my first albino/leucistic race so im amazed at how crazy looking they are! so cool
btw, quick question: I plan to take a few clones from this tub. I have successfully cloned mature mushrooms the 5-6 times ive tried, but i have been reading a lot of people saying that if you are going to take a clone you should do it early, youngest myc possible, ideally one of the first pins (i have no idea about this). Should i wait until the fruits mature and showing characteristics and clone the ones that are most interesting, or should i clone some of these first flush pins now?
thanks again everyone
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23566129 - 08/22/16 02:16 PM (7 years, 5 months ago) |
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Clone a pin from a cluster. Clone a few really. Cluster genetics often produce way better and the young pin will produce a culture with more vigor.
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SuperFly
Still in the Space Race



Registered: 05/19/13
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23566150 - 08/22/16 02:24 PM (7 years, 5 months ago) |
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get hyphy hahaha you're awesome
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c10h12n2o
serial dilutor



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Re: Back in the Saddle / AA+ Pin Porn [Re: SuperFly]
#23566238 - 08/22/16 02:56 PM (7 years, 5 months ago) |
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Quote:
Pastywhyte said: Clone a pin from a cluster. Clone a few really. Cluster genetics often produce way better and the young pin will produce a culture with more vigor.
thanks pasty, makes lots of sense, you da man 
are there any special precautions i should take when removing pins to clone (regarding the selected pin or the rest of the tub)? or just pluck em out with gloved fingers and clone as usual (dip in peroxide, biopsy inside center of stipe, transfer to agar)? or is there a better technique using scalpel or anything else? never cloned a pin before, only mature fruits
Quote:
SuperFly said: get hyphy hahaha you're awesome
lol stay hyphy like keak da sneak my brother
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23569030 - 08/23/16 12:35 PM (7 years, 5 months ago) |
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ok so i made some clones with flame sterilized tweezers and scalpel a la RR's thread, and i have never seen such deep blue bruising maybe its just because of the light colored fruits, but it looked like ink!! hopefully i didnt fuck anything else up too bad, might have caused one cluster's babies to abort
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23569066 - 08/23/16 12:52 PM (7 years, 5 months ago) |
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If ya wanna make an omelet. . .
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c10h12n2o
serial dilutor



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Re: Back in the Saddle / AA+ Pin Porn [Re: Pastywhyte]
#23572692 - 08/24/16 12:24 PM (7 years, 5 months ago) |
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im tryin to make one paula deen style, with butter and sour cream
lol so yeah, i was worried about the couple of pins i might have touched and causing them to abort, looks like i have a much bigger problem, tons of aborts today!!!
i knew all those knots looked a little too good to be true, considering the unlevel substrate and MS spawn, and the fact that its my first tub in quite a while
the clones i took are looking good. Someting really cool though, several of the pins have aborted have gotten super fuzzy and thick at the bottom, like trying to re absorb. and the ones near large fruits are absorbing into the stems of the big ones
is there anything i should do about these at this point? any intervention called for to make the most of the situation? i have lots more tubs in the works, so its not like i am banking on this one or anything, just trying to get some good clones and play around with this race, and most of all learn 

thanks again everyone!! best regards
PS edit: I have another 10 jars of AA+ spawn ready to go today. they were made using LI from limited MS plates, even more limited than the first one because it was further along in the same series of transfers. I am trying to decide between just doing another 2-3 monotubs with them or putting them into trays (plastic 5qt shoeboxes) and fruiting them in a GH.
my main objective for these is just to obtain some good clones and sharpen up my technique before i start my serious projects, testing all these isolates i have been working on. I have 6 other races of MS colonizing in tubs right now, all for the same purposes. so ideally i would like to be practiced up to refine and standardize the methods i will be using to test all the important stuff. in light of what i am tryin to do, what makes the most sense?
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (08/24/16 12:43 PM)
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Rooster Cogburn
Ranger

Registered: 07/19/16
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23572863 - 08/24/16 01:38 PM (7 years, 5 months ago) |
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Very nice Looking beautiful, as much as I want to kick back and watch you guys grow these magnificent beasts, I need to get on this AA+ train.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
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i'll PM you good buddy
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23579544 - 08/26/16 11:24 AM (7 years, 5 months ago) |
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UPDATE:
harvested yesterday, all but a couple that i will harvest today
total wet weight harvested out of this first flush so far is 1097g
cant wait to start working with clones and isolates 

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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Rooster Cogburn
Ranger

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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23579786 - 08/26/16 12:25 PM (7 years, 5 months ago) |
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Fuck yeah Fill your hands with that you son of a bitch!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Nice job man.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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concretelush
shadow cast light mass


Registered: 08/22/16
Posts: 251
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23580575 - 08/26/16 03:46 PM (7 years, 5 months ago) |
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Rooster Cogburn
Ranger

Registered: 07/19/16
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Re: Back in the Saddle / AA+ Pin Porn [Re: concretelush]
#23580659 - 08/26/16 04:02 PM (7 years, 5 months ago) |
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Quote:
Ned Pepper: I call that bold talk for a one-eyed fat man. Rooster Cogburn: Fill your hand, you son of a bitch! It was too good of a line to be done over again, I bet that was a one take liner too! Hey ever seen the sequel, just called "Rooster Cogburn" Katharin Hepburn plays a nun that he's escorting, it's fucking hilarious.
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c10h12n2o
serial dilutor



Registered: 01/21/15
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hahahaha what a badass.... i had no idea! i will definitely check it out! he was such a great character
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23588705 - 08/28/16 10:43 PM (7 years, 5 months ago) |
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UPDATE:
first flush dry weight turned out to be 124g 
not bad for MS and my first tub in yrs, but there are much better things coming, i can promise you that 
clones on agar looking good so far
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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c10h12n2o
serial dilutor



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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23719664 - 10/08/16 02:16 PM (7 years, 3 months ago) |
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UPDATE:
holy jesus it has been a busy summer!!!! so fun though So i have been working towards isolates and doing tons of agar stuff, working with lots of varieties and species. but just wanted to post an update on what became of this project
just harvested a tub that was a clone from this first AA+ monotub, incredible fruit size and weight!! had one monster that weighed almost 300g by itself! total harvest was 3,496 grams first flush off of 1 66qt tub (!!!)
   
pretty sweet!!! i know its bad form to clone a clone, but i might have to clone that monster. there were still lots of strains present in this culture, i have done an additional 7 transfers trying to isolate from it
tub was coir, verm, hpoo, gypsum, & straw. really digging the AA+ genetics ive been working with, cant wait to start testing monocultures and colecting some data points
here is another clone AA+ tub (different culture) just now starting to pin:

thanks to everyone for all the great info on the forum (that means you bodhi, pasty, inoc, etc)!
warm regards
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23719684 - 10/08/16 02:22 PM (7 years, 3 months ago) |
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3496 grams first flush on a 66 quart tub is damn fine work. That's a real beast ya go there, personally I would try and keep it going for sure.
Nice work
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23720057 - 10/08/16 04:47 PM (7 years, 3 months ago) |
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Quote:
c10h12n2o said: UPDATE:
holy jesus it has been a busy summer!!!! so fun though So i have been working towards isolates and doing tons of agar stuff, working with lots of varieties and species. but just wanted to post an update on what became of this project
just harvested a tub that was a clone from this first AA+ monotub, incredible fruit size and weight!! had one monster that weighed almost 300g by itself! total harvest was 3,496 grams first flush off of 1 66qt tub (!!!)
   
pretty sweet!!! i know its bad form to clone a clone, but i might have to clone that monster. there were still lots of strains present in this culture, i have done an additional 7 transfers trying to isolate from it
tub was coir, verm, hpoo, gypsum, & straw. really digging the AA+ genetics ive been working with, cant wait to start testing monocultures and colecting some data points
here is another clone AA+ tub (different culture) just now starting to pin:

thanks to everyone for all the great info on the forum (that means you bodhi, pasty, inoc, etc)my greatest yield in a
warm regards
3496.......
My greatest yield on a 66qt mono has been 2653.
Spectacular job Serotonin!
A+ stuff!
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
#23720088 - 10/08/16 04:58 PM (7 years, 3 months ago) |
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Quote:
morty422 said: A+ stuff!
AA+ stuff
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Re: Back in the Saddle / AA+ Pin Porn [Re: mushboy]
#23720091 - 10/08/16 05:02 PM (7 years, 3 months ago) |
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Quote:
mushboy said:
Quote:
morty422 said: A+ stuff!
AA+ stuff
Damn you!
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ChristaphonZ


Registered: 09/10/16
Posts: 19
Last seen: 6 years, 7 months
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Re: Back in the Saddle / AA+ Pin Porn [Re: morty422]
#23720798 - 10/08/16 09:56 PM (7 years, 3 months ago) |
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Nice job!
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
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Re: Back in the Saddle / AA+ Pin Porn [Re: mushboy]
#23720814 - 10/08/16 10:04 PM (7 years, 3 months ago) |
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Quote:
Pastywhyte said: 3496 grams first flush on a 66 quart tub is damn fine work. That's a real beast ya go there, personally I would try and keep it going for sure.
Nice work 
thanks pasty! i very much appreciate it, i think you personally deserve at least half the credit, no shit. between you, azure, bodhi, frank, and of course RR (peace be upon him), the rest of us dont really have any excuses left to have shitty grows lol
i will definitely clone it, damn thing looks like something from No Man's Sky... some KSSS lookin characteristics.
I also got super lucky today and FINALLY had a PE fruit drop spores!!! theyve all been sterile so far, but this one is printing like a normal fruit 
Quote:
morty422 said: 3496.......
My greatest yield on a 66qt mono has been 2653.
Spectacular job Serotonin!
A+ stuff!
Thanks buddy!! more than happy to send you a print, this line of AA+ has been the print that just keeps on giving, have lots of promising cultures in the works
i think my 2nd best was just over 2kg, like 2003g or something... i thought my scale was fucked at first (i checked) but then i looked at the MOUNTAIN in front of me and it was confirmed. did keep expecting to wake up from a dream though, especially with the PE print... been dreaming about agar and tubs a lot lately lol
Quote:
mushboy said:
Quote:
morty422 said: A+ stuff!
AA+ stuff
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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wtfcrazymofo
foil hater



Registered: 07/26/15
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Re: Back in the Saddle / AA+ Pin Porn [Re: c10h12n2o]
#23721260 - 10/09/16 05:31 AM (7 years, 3 months ago) |
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Quote:
c10h12n2o said: UPDATE:
 thanks to everyone for all the great info on the forum (that means you bodhi, pasty, inoc, etc)!
warm regards
Nice gangly monster and tub. I love that AA+. Albinos? Kind of light I guess.
How much spawn per tub? What was the sub or casing ratio? Did you soak grain after you broke it up before you spawned it?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: Back in the Saddle / AA+ Pin Porn [Re: wtfcrazymofo]
#23722180 - 10/09/16 01:07 PM (7 years, 3 months ago) |
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Quote:
wtfcrazymofo said:
Quote:
c10h12n2o said: UPDATE:
 thanks to everyone for all the great info on the forum (that means you bodhi, pasty, inoc, etc)!
warm regards
Nice gangly monster and tub. I love that AA+. Albinos? Kind of light I guess.
How much spawn per tub? What was the sub or casing ratio? Did you soak grain after you broke it up before you spawned it?
thanks brotha!
yeah leucistic they are a lot lighter before they are covered in spores
I used a spawn bag with probably 4-5 real quarts of wheat berries. Ratio was about 1:2-3. I mix coir, hpoo, gypsum and verm and bring to field capacity, then pasteurize, and at the same time i pasteurize a paint strainer bag full of straw. I use 2 gallon freezer bags of substrate and several handfulls of straw per tub, no casing. And yes i did soak the grain after breaking it up, right in the spawn bag
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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