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2600MHz
Happy


Registered: 04/22/16
Posts: 28
Last seen: 2 years, 5 months
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Total loss of all jars.
#23560011 - 08/20/16 01:40 PM (7 years, 5 months ago) |
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Well, this ain't good. Complete loss of all jars. Rye pastuerized via boiling water into 5gallon bucket tek. Then loaded jars up and 90 minutes at 15psi in a PC. Knocked them up with a flame sterilized syringe in a SAB. Sprayed alcohol and Lysol everywhere. Wore gloves cleaned in iso, face mask and tyvek sleeves worn. Polyfil lids, no filter. Thinking I need to make/buy some filters for the lids. Or self-sealing injection ports. Ah well. I'll be scrapping these tonight and ordering up some more spores. I'm not giving up.


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2600MHz
Happy


Registered: 04/22/16
Posts: 28
Last seen: 2 years, 5 months
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Re: Total loss of all jars. [Re: 2600MHz]
#23561458 - 08/20/16 09:39 PM (7 years, 5 months ago) |
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It just dawned on me how it prolly happened. I never changed my polyfil from the last time I used those lids... Hello face, meet mr. palm.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Re: Total loss of all jars. [Re: 2600MHz]
#23562308 - 08/21/16 08:00 AM (7 years, 5 months ago) |
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I would get rtv and some tyvek (you can even use those sleves.) and make some better lids. It wouldnt be from the last grow as you sterilized the poly when you pcd the jars. What most likely happened is the poly fill allowed the contamination in, or much more likely (as it is all of them) is due to your grain prep. The was not need to pasturize. What i do with rye (which i very rarely use, is "boil and bloom" you need to get those bacterial endospores active before you pc or they can easily survive the pc. Good luck.
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Honestly i always hated the whole polyfill thing. Its its not a monotub i dont use it. Rtv is cheap enough and easy to use, and my lids last years (with regular inspection and maintenance)
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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anthiawe
friendly stranger


Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
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PC at that time should kill whats on your lids. I dont usually clean my lids off, i've heard some dont even clean their jars at all because the PC will kill everything.
-------------------- TEK compendium
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Total loss of all jars. [Re: anthiawe]
#23563876 - 08/21/16 07:19 PM (7 years, 5 months ago) |
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Your grain prep was fine. Your polyfil being re-used wasn't an issue as it would've been sterilized, along with the grains and everything else, in your pressure cooker. Your inoculation technique sounds thorough and I've seen success personally with far less precautions taken.
So let's get to the two likely causes of your contamination.
1) Your syringe may have been contaminated from the start. Considering every jar presented with the same contamination with a rate of zero percent success, this would be the most likely cause.
2) Your polyfil isn't stuffed tight enough, allowing contaminants into your jars.
Did you let your PC cool down and return to pressure naturally or did you pull the weight off while still under pressure? Pulling the weight can cause filter failure and also dry your grains out.
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nooneman


Registered: 04/24/09
Posts: 14,568
Loc: Utah
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Re: Total loss of all jars. [Re: 2600MHz]
#23563987 - 08/21/16 07:52 PM (7 years, 5 months ago) |
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You might consider going from agar to grain rather than MS to grain.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Total loss of all jars. [Re: nooneman]
#23564349 - 08/21/16 09:43 PM (7 years, 5 months ago) |
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I don't know where you got your spore syringe, but I suggest sourcing it from the best place possible for future endeavors.
And yes, taking all spores to agar first is a wise technique.
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2600MHz
Happy


Registered: 04/22/16
Posts: 28
Last seen: 2 years, 5 months
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I let the PC cool down overnight while I slept. Cool down was around 9-10 hours and the PC was cool to the touch. Syringe was from a trusted vendor listed on this site. I hadn't considered the syringe, due to me flaming it red hot before knocking up each jar. Also wiped with iso prior to use. Iso on gloves as well. Waited a sec for evap and flamed it.
Getting the poly thru the 1/4" hole in the lid was a living hell. I used a crochet hook needle to pull the material thru the hole. That's the last time I'll ever do that again. I'm planning on tyvek and self-sealing injection ports for the next go-round.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Re: Total loss of all jars. [Re: 2600MHz]
#23566871 - 08/22/16 06:21 PM (7 years, 5 months ago) |
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Do it. You can use the rtv with coincidence the butyl ones are nice but imho not better enough on my budget
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Its the bacterial endospores wgat was the grain prep like. Its something thats consistent thru them all so its the spawn or its the spores.
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Just pasteurized? Did you let the endospores "bloom" or whatever shroomerites calls it? It looks just like ehat i saw my 1st time with grain
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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2600MHz
Happy


Registered: 04/22/16
Posts: 28
Last seen: 2 years, 5 months
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I put down something incorrect in the first post above. The boiling water into bucket was how I handle my monotub substrate mixture. Apologize for the confusion.
For the grain, I rinsed them in a huge bowl to get any of the loose crap out, then left them to soak, completely submerged in hot water and some gypsum for 24 hours. Then that whole mixture went into a pot and was boiled for 10-15 minutes, drained and left to cool on cookie sheets. I used the 10 seconds on a paper towel to gauge moisture. Once it passed the test, they were loaded into the quart jars with the polyfil lids, covered 2x with tinfoil and ran 90 minutes at 15 psi in the PC. Left the PC to cool for around 10 hours,cool to the touch. Foil removed, sprayed everything and wiped everything, etc.. Knocked up in SAB and left to colonize.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Re: Total loss of all jars. [Re: 2600MHz]
#23569012 - 08/23/16 12:25 PM (7 years, 5 months ago) |
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Idk man sounds like shit luck, under those conditions idk what could have happened. That leaves the poly or the syringe, where was the syringe sourced from?
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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2600MHz
Happy


Registered: 04/22/16
Posts: 28
Last seen: 2 years, 5 months
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Quote:
KThunderland said: Idk man sounds like shit luck, under those conditions idk what could have happened. That leaves the poly or the syringe, where was the syringe sourced from?
From what I understand, we shouldn't mention the exact name if a product was bought from a trusted vendor. My source is listed on the Sponsors page and has been part of the community for 12 years. I don't expect they did anything wrong. I've ordered from them before and have had no issues. If anything, it had to have been me.
--------------------
 Damion 5050 coir tek
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Re: Total loss of all jars. [Re: 2600MHz]
#23569264 - 08/23/16 01:54 PM (7 years, 5 months ago) |
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Thats what i was asking in essence. Are you in the states ill send you one of my used (but plenty reuseable) homemade tyvek and rtv lids for free (if youd wanna send stamps or a print when life allows that would be cool too) are you using wide mouth?
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Actually ill make a new one even.
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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2600MHz
Happy


Registered: 04/22/16
Posts: 28
Last seen: 2 years, 5 months
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Quote:
KThunderland said: Thats what i was asking in essence. Are you in the states ill send you one of my used (but plenty reuseable) homemade tyvek and rtv lids for free (if youd wanna send stamps or a print when life allows that would be cool too) are you using wide mouth?
Thanks for the offer friend. I already have an order placed online for that and a couple other things. I really appreciate your kindness.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Re: Total loss of all jars. [Re: 2600MHz]
#23571949 - 08/24/16 06:14 AM (7 years, 5 months ago) |
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No problem , were all here for the same reasons.
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Quote:
KThunderland said: Its the bacterial endospores wgat was the grain prep like. Its something thats consistent thru them all so its the spawn or its the spores.
Bacterial endospores do not cause mold.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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I cant enlarge for some reason , i thought i was looking at slime. If it is indeed mold then the poly is letting something in.
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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Always sucks to lose jars, If you need some more spores pm me i just made a batch of prints i can get one to you and you can get a do over, just pay it foward.
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Quote:
If it is indeed mold then the poly is letting something in.
Or his syringe was contaminated. Could be either, but I'm leaning towards the syringe being contaminated considering how tight the OP said he stuffed the GE holes.
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KThunderland
Dude


Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 2 months
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True, toss thwm or make a plate, loose ply will rarely contam the entire lot the same
-------------------- Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.
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