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Offlinemushroomnoob1981
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Registered: 06/15/15
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Bad syringe or bad technique?
    #23559869 - 08/20/16 12:40 PM (7 years, 6 months ago)

So I knocked up some rye with some MS syringes. My jars are sealed with red silicone self-healing injection ports,  so you don't have to open the jar to inject.

What I did

-flamed the needle till red hot
-wiped the top of the like with IPA
-injected 1 cc each through the ports

Almost all of them came back contaminated. I also had "control" jars (same type of jars,  PCd for the same amt of time,  same prep,  etc...). These jars did not get injections and they did not develop molds.

You think it's a technique problem or a contaminated syringe?  I didn't use a glove box because I only inserted the red hot section of the needle through the ports. I'd figure anything in there is long dead.

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InvisibleMad Season
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Re: Bad syringe or bad technique? [Re: mushroomnoob1981]
    #23559883 - 08/20/16 12:45 PM (7 years, 6 months ago)

Sounds like syringe if almost every jar contams


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InvisibleThadeous
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Re: Bad syringe or bad technique? [Re: Mad Season]
    #23560046 - 08/20/16 01:56 PM (7 years, 6 months ago)

:whathesaid:

I do my inoculations in open air as well. Very rarely do I get a contam unless it's the whole batch

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InvisibleScrimshaw
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Registered: 06/27/14
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Re: Bad syringe or bad technique? [Re: Thadeous]
    #23560092 - 08/20/16 02:23 PM (7 years, 6 months ago)

Did the contam show in the area you injected? It sounds like a bad syringe.

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OfflineKenetic
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Re: Bad syringe or bad technique? [Re: Scrimshaw]
    #23560128 - 08/20/16 02:46 PM (7 years, 6 months ago)

The last several syringes Ive used (from a reputable sponsor) have been contaminated with bacteria.  No amount of good technique can overcome a bad syringe.  That's why I finally started to learn agar (and I still have a looooooong way to go lol).

If you want more control over your grow you will definitely benefit from agar as well, and it's fun as hell too!  I can't imagine any other way now.

I'm sorry if I missed this but did you use a sab?  Even if your syringe was clean (which it doesn't seem like), a brief exposure to open air could allow contams on your SHIP, and since alcohol doesn't sterilize you "could have" pushed the contams through that with the needle.  I doubt it though.  I used to use  similar method as you in open air and I had a lot of success.

Agar, homeskillet.  If I can learn it, anyone can.


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