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OfflineZeroCool
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Registered: 12/27/15
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Stones growing in agar?
    #23557833 - 08/19/16 06:32 PM (7 years, 5 months ago)

So I started my first agar jars a couple days ago and I accidentally squirted  a bunch of spores instead of doing one drop (oops) so this particular jar had a bunch of different isolates in it, and they eventually made one huge mass that hasn't quite covered the entire jar yet, but its quite a bit. I looked at it in my glovebox when I was doing transfers over to new agar and decided that it was contam or something, so I set it aside to see what would happen. I'm glad I didn't throw it out, but I'm still not sure if its contaminated or not. Some of my sectors have these long hairs sticking out the center. underneath all that mycelium are stones, but not sure if that mycelium is healthy or not.



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Edited by ZeroCool (12/28/18 06:40 PM)


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Offlinemrmazdarx9
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Registered: 05/15/16
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Re: Stones growing in agar? [Re: ZeroCool]
    #23557840 - 08/19/16 06:35 PM (7 years, 5 months ago)

Quote:

ZeroCool said:
So I started my first agar jars a couple days ago and I accidentally squirted  a bunch of spores instead of doing one drop (oops) so this particular jar had a bunch of different isolates in it, and they eventually made one huge mass that hasn't quite covered the entire jar yet, but its quite a bit. I looked at it in my glovebox when I was doing transfers over to new agar and decided that it was contam or something, so I set it aside to see what would happen. I'm glad I didn't throw it out, but I'm still not sure if its contaminated or not. Some of my sectors have these long hairs sticking out the center. underneath all that mycelium are stones, but not sure if that mycelium is healthy or not.





Sorry for my ignorance but how can you squirt a load of spores and have isolates?


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OfflineZeroCool
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Re: Stones growing in agar? [Re: mrmazdarx9]
    #23557861 - 08/19/16 06:39 PM (7 years, 5 months ago)

not even sure, it was more than a drop, that's for sure. Way more. Doesn't help that I accidentally tilted the jar every which way as well.  :facepalm3:


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OfflineAgarStudent
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Re: Stones growing in agar? [Re: ZeroCool]
    #23559595 - 08/20/16 10:22 AM (7 years, 5 months ago)

I have done the same thing with a particular strain called Chico Nindo, which is a subspecies of the Mexicana.  I guess when you apply pressure to the syringe then a lot can squirt out and they are not the best things for controlled dropping!



I seem to be getting a similar thing going where there are two spots of yellow (possible stones) or areas of increased density.  The areas of possible stone formation are at the edges of the dish, where only one sector had to compete for nutrients -

Hence, I don't actually know if the stone formation is due to the substrain (sector) not having to compete with other sectors for nutrients or if it is indeed a substrain that inherently forms stones and should be transferred?

So what do you do? transfer the yellow stoney looking bit to another plate or transfer the healthiest fast-growing white fluffy mycelium?  It seems like a black art sometimes?


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OfflineZeroCool
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Re: Stones growing in agar? [Re: AgarStudent]
    #23559963 - 08/20/16 01:22 PM (7 years, 5 months ago)

I think I'm going to eventually try and transfer the stones to different plates and see how that turns out. I think its pretty cool how they were able to form so quickly.


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Offlineblackout
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Re: Stones growing in agar? [Re: AgarStudent]
    #23562146 - 08/21/16 05:21 AM (7 years, 5 months ago)

Quote:

AgarStudent said:
I guess when you apply pressure to the syringe then a lot can squirt out and they are not the best things for controlled dropping!





these are some tips on syringe control I posted before.



Quote:

blackout said:
Quote:

third3y3 said:
It's SOOO easy to squeeze too much.  I guess I'll get better. Typical newbie type mistake.



Theres a trick to stop this, do not use the syringe like people typically do. I am right handed, I grasp the syringe in my right hand making a thumbs up sign, the plunger end is sticking up with my thumb under the bit you usually press down with your finger. So if I gave a thumbs up out straight the needle is facing the floor.

My right pinky finger is gripping the body of the syringe at the 10ml sign

Now you can press down the plunger with your left hand while actively pressing upwards with your right thumb. This gives you good control. If a syringe is unused for a while the rubber seal will be subject to static fritcion (aka stiction). If you use a syringe in a normal manner the moment this stiction is overcome the plunger will tend to move far too much due to the force you are applying and the fact nothing is stopping the syringe from going all the way down.

You can do it with just 1 hand too, just practice with water.






Quote:

blackout said:

I didn't state the obvious, but as the syringe empties obviously my pinky finger moves down the body with the print on it. You can get decent control with just 1 hand too, I usually only use the 2 hand method with a syringe which has not been used in a long time. With the 1 hand method I clench my fist around the syringe. When full my little finger wrapped around the syringe body (with the print) and my other 3 fingers grasp the plunger shaft firmly and my thumb presses down. So the plunger shaft is stopped from moving quickly by the friction of my fingers. As the liquid gets used up I put the next finger on the printed body. For the last 2ml my forefinger is just pressed against the underside of the plunger shaft which again just stops it being able to jump down too much.

Another 1 handed method is having your 3 fingers grasp the body with the printing, and your forefinger wrapped around the plunger shaft which provides resistance. Then press down with your thumb in a controlled manner. As said, just practice with plain water and see what suits you best.




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OfflineAgarStudent
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Re: Stones growing in agar? [Re: blackout]
    #23562372 - 08/21/16 08:45 AM (7 years, 5 months ago)

Question -

If you just transplant a little chunk of fast-growing mycelium of a scerlotia producing strain to grains, then will the jar still grow stones or will it just end up as mycelium?


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