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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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While We are Talking SABs...
#23557396 - 08/19/16 04:21 PM (7 years, 5 months ago) |
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I've noticed quite a bit of SAB / GB discussion of late, so i figured i would share some of mine, for the benefit of anyone trying to do the same.
Also, I would very much appreciate any feedback/advice/violent hate from you vets out there 
I recently got back into cultivation and wanted to do it right this time, using what i have learned over the years and approaching it seriously. Since I was to be doing lots of agar work this time around, i wanted a good glove box. After a bit of digging, i found this killer link for a great double hatch build: nice glove box plans
I made several modifications to this plan, especially since my arms are too big to fit into ANY of the PVC pipes i can find in stores, and the force of putting on gloves was too much for the plastic, and i added weather stripping and a nonstick mat:

This setup has served me surprisingly well. I have had virtually no contamination in this thing, only an occasional speck in an agar plate (small enough that i would have overlooked it in years past), and never anything that isnt easily avoided during transfers, if ever i chose one of those plates for transfers.
My main issue is i get VERY hot and sweaty when doing a few sleeves of plates, uncomfortably so. This has made me lust for the cool breeze of a flow hood in my face, as has the desire to take better photos of agar plates.
I am rounding up parts for a flow hood, but in the meantime my gf wants all this shit out of the kitchen!! So in rearranging my grow space, noticed a little closet in a room with hardwood floors, and i started to visualize a tailor-made SAB that is out of the way and has the benefit of 3 walls. While i am thinking this over, i drive past a HUGE sheet of broken plexiglass someone was throwing away, so i busted a u-turn and begun planning the box on SketchUp (which i am not good at)

I wanted to build it in a way that the inside surfaces were as flat as possible (rather than having the supports on the inside), to make it easy to clean/wet the sides before use. I also wanted to use mold resistant paint and have a large door or hatch for this purpose, and for transporting large things in and out. I also sealed it with mold resistant caulk and used rubber gaskets to keep from further damaging the free plexiglass. if/when it fails i will upgrade to glass or real plexiglass.


I just finished it up and am about to put it to the test 
I am considering adding a UV-C fixture behind the LED , and using a remote to turn it on and off. That way, when i want to use the box, i can close the closet tight, turn on the UV-C light for 10-15 minutes, turn it off, then get to work . What do yall think about this idea? obviously i wouldn't depend on it, as clean technique the only safe bet, just an extra precaution for 15$. And i know SAB functions based on still air, not sterility; i have had great success with my last SAB doing agar work, and welcome even a slight improvement
Any feedback is much appreciated
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: While We are Talking SABs... [Re: c10h12n2o]
#23557424 - 08/19/16 04:29 PM (7 years, 5 months ago) |
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Uv-c will just make your plastic brittle and not as clear. Don't rely on stupid crutches the box does a 100x better job than the light will to keep your work clean if you have the skills to go along with.
I make my arm holes big enough to just move jars and shit in through those holes.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
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Re: While We are Talking SABs... [Re: bodhisatta]
#23557440 - 08/19/16 04:35 PM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: I make my arm holes big enough to just move jars and shit in through those holes.
I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes  Although looking at my last plates you would be forgiven for thinking that I hadn't shit on the plates they were that bad
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Re: While We are Talking SABs... [Re: mrmazdarx9]
#23557513 - 08/19/16 05:01 PM (7 years, 5 months ago) |
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Quote:
mrmazdarx9 said: I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes 
Fuck, that was pretty funny.
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mrmazdarx9
Pffffttt


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Re: While We are Talking SABs... [Re: Inocuole]
#23557523 - 08/19/16 05:04 PM (7 years, 5 months ago) |
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Quote:
Inocuole said:
Quote:
mrmazdarx9 said: I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes 
Fuck, that was pretty funny.
Was an easy one tbh lol
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: While We are Talking SABs... [Re: mrmazdarx9]
#23557539 - 08/19/16 05:07 PM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: Don't rely on stupid crutches the box does a 100x better job than the light will to keep your work clean if you have the skills to go along with.
.... thats why i said Quote:
obviously i wouldn't depend on it, as clean technique the only safe bet, just an extra precaution for 15$
no "depending on crutches" involved, except maybe your reading comprehension.
the two certainly arent mutually exclusive. I have had great results for years by using good clean technique and still air. I dont think addint a 10 second UV-C cycle is going to nullify proper still air or proper clean tech, certainly not a crutch, if anything it might help, thats why wanted to discuss it.
would plexiglass be degraded that quickly? of course i would expect some major damage to paint and plastic if it was run 24/7, but 10 minutes every 2-3 days?
Quote:
mrmazdarx9 said:
Quote:
Trusted Cultivator said: I make my arm holes big enough to just move jars and shit in through those holes.
I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes 
lol
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: While We are Talking SABs... [Re: bodhisatta]
#23557553 - 08/19/16 05:10 PM (7 years, 5 months ago) |
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If I ever build another SAB I won't have arm holes just one big ass oval in the front
Also I read your post just fine, even that next sentence... I could care less. If you say uv-c you get my uv-c standard opinion. Which you dont have to listen to, especially not get asshurt about either.
Most real labs have moved away from using UV-C stages on their hoods I can only imagine the reasoning is because it's a gimmick in that particular application
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: While We are Talking SABs... [Re: bodhisatta]
#23557587 - 08/19/16 05:20 PM (7 years, 5 months ago) |
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your standard uvc treatment is usually in response to someone trying to pasteurize or sterilize bulk substrate, or grains, or something else that would be trying to rely on it instead of proper tech. Clearly this is a different context
no anus injuries here, you just take things out of context quite often and i needed to call you out about it to keep the conversation on point, and distinguish it from your "standard opinion" about another use in another context
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (08/19/16 05:21 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: While We are Talking SABs... [Re: c10h12n2o]
#23557590 - 08/19/16 05:22 PM (7 years, 5 months ago) |
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People like to avoid the advice they know they are going to get by adding the
"I know it's unnecessary/overkill/dumb "
Only makes me more eager to post the thing that's going to make you say that I don't know how to read

I'm not the one asking about bullshit
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: While We are Talking SABs... [Re: bodhisatta] 1
#23557688 - 08/19/16 05:49 PM (7 years, 5 months ago) |
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because nothing screams lack of bullshit like someone christening themselves with a DIY Trusted Cultivator tag 
anyways, any actual discussion about using a supplemental uv-c cycle in this type of SAB is much welcome. as a supplement to proper sterilization/pasteurization & clean tech, NOT as a substitute for any of these. some of us actually enjoy thinking about these things, irrespective of any kind of endorsement
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (08/19/16 05:54 PM)
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: While We are Talking SABs... [Re: c10h12n2o]
#23557724 - 08/19/16 05:59 PM (7 years, 5 months ago) |
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Oh sure, sure! Snub the man that spends 50% of his time at work in a laboratory.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: While We are Talking SABs... [Re: c10h12n2o] 1
#23557745 - 08/19/16 06:06 PM (7 years, 5 months ago) |
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sorry I took the most obviously awesome username available hah
so you'll try UVGI in your SAB. you'll load up all your jars, and dishes etc... then you'll turn the lamp on for 10-15m but there's the line of sight problem. so you'll have to go and rotate your shit around a few times or just the one side gets exposed.
you won't want to put in any of your spores petris, or inoculum in there because you don't want to damage it's DNA before using it.
then you'll turn it off (or forget to and get some sunburn, yes I know it's longer UV wavelengths that cause that in humans, but that's because the short ones don't make it down to the surface of the planet) the short wave UVC is actually worse for the skin and causes sunburn faster.
then with the lamp off you have to put your inoculant, dishes, spores back into the SAB which effectively will undo the cleaning the UVC did
you'll still have to wipe down everything in alcohol which is just a sanitizer anyway. bringing you back exactly to square one before you turned the lamp on.
you'll spray the SAB down before using it, but you'll have to remember to clean your bulb every time since residue, film, hard water deposits, dust, etc... greatly diminish the effectiveness of the bulb
then there's the degradation of your plastics including the SAB and anything else UV sensitive.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: While We are Talking SABs... [Re: bodhisatta]
#23557750 - 08/19/16 06:08 PM (7 years, 5 months ago) |
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There are way shittier cultivators on this site with real tags.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: While We are Talking SABs... [Re: Inocuole]
#23557758 - 08/19/16 06:12 PM (7 years, 5 months ago) |
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Quote:
Inocuole said: There are way shittier cultivators on this site with real tags.
well to be fair some of them are not active anymore,
some of them are though
blackout CH HELL eatyualive george castanza hamloaf tahoe _Otto_
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: While We are Talking SABs... [Re: bodhisatta] 1
#23557763 - 08/19/16 06:13 PM (7 years, 5 months ago) |
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I see 3-4 mediocre cultivators and then a few decent cultivators that you don't like.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: While We are Talking SABs... [Re: Inocuole]
#23557792 - 08/19/16 06:20 PM (7 years, 5 months ago) |
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those other 3-4 are plenty fine at growing mushrooms but their advice is garbage
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bodhisatta 
Smurf real estate agent


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Re: While We are Talking SABs... [Re: bodhisatta]
#23557795 - 08/19/16 06:21 PM (7 years, 5 months ago) |
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one particular person that reminds me of the days in the year almost made that list for a shumvbox
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: While We are Talking SABs... [Re: c10h12n2o]
#23557926 - 08/19/16 06:59 PM (7 years, 5 months ago) |
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dear OP,
there is no need to wrap your minitubs with syran wrap. in fact, I would advise against that.
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PussyFart
Retired Cultivation Extrodinaire



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Re: While We are Talking SABs... [Re: bodhisatta] 1
#23557936 - 08/19/16 07:03 PM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: one particular person that reminds me of the days in the year almost made that list for a shumvbox
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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c10h12n2o
serial dilutor



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Re: While We are Talking SABs... [Re: Munchauzen]
#23557959 - 08/19/16 07:12 PM (7 years, 5 months ago) |
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good info bodhi, though its a little different than what i had in mind. and also, i have been having the same level of success just using soap and water (as per RR suggestions) as i did with bleach and/or iso. what i had in mind was to soap/wet the sides and then run it 10 minutes in the empty box (shut in the closet, using a remote), and then proceed as normal. i dont see how it would be any more trouble than usual when used in this way, and searching academic databases there are dozens of examples of uv-c light being used in exactly this way. anyways, it was just a thought, certainly not some "tek" i advocate, just talking points
lol and not snubbing anyone, i was just pointing out that the question was certainly not bullshit. context is very important... and bodhi isnt the only person who spends at least 50% of their time in a lab, thats part of what makes this place so great but correct ideas are what matters, not ego or personalities
and i totally agree inocuole, without a doubt 
Re Munch: those are actually not mini tubs, they are trays that are colonizing. but I very much appreciate the advice!! does that change anything? i have 5 holes punched in the wrap with micropore tape over each. much obliged my friends
Edited by c10h12n2o (08/19/16 07:23 PM)
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Mycolorado
Hobbyist


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Re: While We are Talking SABs... [Re: c10h12n2o]
#23588031 - 08/28/16 07:05 PM (7 years, 5 months ago) |
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Whoa, I must be breaking all the rules with this setup. My buddy built this a couple years back out of a DIY mag. I run the filter before or even sometimes while working in it. I'll run the light over freshly poured plates or plastic containers to sterilize them. Not sure what all the fuss is about. If something works and people have fun tinkering then I say right on. I'll use this til I build a proper flow bench.
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Kenetic
Nam Sayin



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Re: While We are Talking SABs... [Re: Mycolorado]
#23588116 - 08/28/16 07:32 PM (7 years, 5 months ago) |
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-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: While We are Talking SABs... [Re: Mycolorado]
#23588319 - 08/28/16 08:34 PM (7 years, 5 months ago) |
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Quote:
Mycolorado said: Whoa, I must be breaking all the rules with this setup. My buddy built this a couple years back out of a DIY mag. I run the filter before or even sometimes while working in it. I'll run the light over freshly poured plates or plastic containers to sterilize them. Not sure what all the fuss is about. If something works and people have fun tinkering then I say right on. I'll use this til I build a proper flow bench.

that's basically a shumvbox. something only a couple(literally two) people say they use with success. (but realistically have ran into contamination problems)
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Psilosoulful

Registered: 09/05/14
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Re: While We are Talking SABs... [Re: bodhisatta]
#23588338 - 08/28/16 08:42 PM (7 years, 5 months ago) |
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Re: While We are Talking SABs... [Re: Psilosoulful]
#23588347 - 08/28/16 08:44 PM (7 years, 5 months ago) |
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Quote:
Psilosoulful said:

You drunk when you made that, weren't you? ..Hey whatever gets the job done..
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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I keep the right one a touch lower. Some of us have got preferences. But honestly, his looks nicer than mine.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
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Re: While We are Talking SABs... [Re: dankington]
#23588390 - 08/28/16 08:54 PM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said:
Quote:
Mycolorado said: Whoa, I must be breaking all the rules with this setup. My buddy built this a couple years back out of a DIY mag. I run the filter before or even sometimes while working in it. I'll run the light over freshly poured plates or plastic containers to sterilize them. Not sure what all the fuss is about. If something works and people have fun tinkering then I say right on. I'll use this til I build a proper flow bench.

that's basically a shumvbox. something only a couple(literally two) people say they use with success. (but realistically have ran into contamination problems)
that light sab talker uses burns my eyes.Quote:
dankington said:

I keep the right one a touch lower. Some of us have got preferences. But honestly, his looks nicer than mine.
badass simplistic crook style sab
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
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Re: While We are Talking SABs... [Re: bodhisatta]
#23588630 - 08/28/16 10:19 PM (7 years, 4 months ago) |
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Quote:
Trusted Cultivator said:
Quote:
Mycolorado said: Whoa, I must be breaking all the rules with this setup. My buddy built this a couple years back out of a DIY mag. I run the filter before or even sometimes while working in it. I'll run the light over freshly poured plates or plastic containers to sterilize them. Not sure what all the fuss is about. If something works and people have fun tinkering then I say right on. I'll use this til I build a proper flow bench.

that's basically a shumvbox. something only a couple(literally two) people say they use with success. (but realistically have ran into contamination problems)
Hasn't everyone had a contam here and there?
Edited by Mycolorado (08/28/16 10:20 PM)
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Re: While We are Talking SABs... [Re: Mycolorado]
#23588647 - 08/28/16 10:24 PM (7 years, 4 months ago) |
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Quote:
Mycolorado said: Quote:
Trusted Cultivator said:
Quote:
Mycolorado said: Whoa, I must be breaking all the rules with this setup. My buddy built this a couple years back out of a DIY mag. I run the filter before or even sometimes while working in it. I'll run the light over freshly poured plates or plastic containers to sterilize them. Not sure what all the fuss is about. If something works and people have fun tinkering then I say right on. I'll use this til I build a proper flow bench.

that's basically a shumvbox. something only a couple(literally two) people say they use with success. (but realistically have ran into contamination problems)
Hasn't everyone had a contam here and there?
Yes. So what's your point? Ever heard of the KISS principle? (Keep It Simple, Stupid)
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Tuhdoww
Sub Slapper


Registered: 08/23/16
Posts: 300
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Almost lit myself on fire using my glove box the other day. Went to flame my needle and my alcohol rag was fuming so hard it caught on fire from across the box. Jumped up with my arms still in the thing looking like a fool. I'm was shitting bricks. Fire alarms going off at 7am in the house. My wife woke up so pissed lol. Moral of the story, Sab from now on , keeping all flames outside of box, 90% iso is flammable. Derp
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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lol love the drunk SABs 
btw, i have been really digging the one i built in the OP, it has made my life so much easier able to do way more work faster. the first one worked well too, but my big ass cant hardly fit in it comfortably, my custom built job is way more comfy, and nicely concealable in the closet, plus the closet adds 5 extra walls, similar to the grow tent frank uses sometimes. so yeah now my gf is happy to have the kitchen back haha
the SAB was supposed to be the main topic of the thread, the possibility of adding a UV-C fixture was just an afterthought for talking points, but saying the word "UV-C" set off bodhi's bullshit detector, which became most of the thread. if you are familiar with some of the other posts about UV-C that bodhi has answered, it is easy to understand why his bullshit detector is a bit oversensitive regarding this technology, considering how many truly bullshit applications for it he has addressed and tried to set people straight on
btw really cool box, thanks for sharing 
fyi, your using the light a little differently than i was talking about though, since i was only discussing the possibility of running a cycle in a closed closet before loading it, just as an extra step along with wetting the walls. just clarifying 
i dont think theres anything wrong with supplementing good technique with a short UV-C cycle, but it depends on what you are trying to do, and it certainly isnt a replacement for proper tech, and depending on what you are trying to do, it is probably a waste of time. but used properly and in the right context, might be helpful
thanks for sharing your notes and pics , warm regards
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
Psychedel.EXE said:
Quote:
Psilosoulful said:

You drunk when you made that, weren't you? ..Hey whatever gets the job done..
Haha fuck you
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 7 minutes
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Re: While We are Talking SABs... [Re: c10h12n2o]
#23589164 - 08/29/16 06:39 AM (7 years, 4 months ago) |
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Quote:
c10h12n2o said: lol love the drunk SABs 
btw, i have been really digging the one i built in the OP, it has made my life so much easier able to do way more work faster. the first one worked well too, but my big ass cant hardly fit in it comfortably, my custom built job is way more comfy, and nicely concealable in the closet, plus the closet adds 5 extra walls, similar to the grow tent frank uses sometimes. so yeah now my gf is happy to have the kitchen back haha
the SAB was supposed to be the main topic of the thread, the possibility of adding a UV-C fixture was just an afterthought for talking points, but saying the word "UV-C" set off bodhi's bullshit detector, which became most of the thread. if you are familiar with some of the other posts about UV-C that bodhi has answered, it is easy to understand why his bullshit detector is a bit oversensitive regarding this technology, considering how many truly bullshit applications for it he has addressed and tried to set people straight on
btw really cool box, thanks for sharing 
fyi, your using the light a little differently than i was talking about though, since i was only discussing the possibility of running a cycle in a closed closet before loading it, just as an extra step along with wetting the walls. just clarifying 
i dont think theres anything wrong with supplementing good technique with a short UV-C cycle, but it depends on what you are trying to do, and it certainly isnt a replacement for proper tech, and depending on what you are trying to do, it is probably a waste of time. but used properly and in the right context, might be helpful
thanks for sharing your notes and pics , warm regards
Do you run the uvc when you are doing your work? If so do you need safety glasses? Your sab is a Caddy. If you want comfortably flow hoods are where its at bro. I'm 1 $250 hepa filter away. My sab is as ghetto as it gets, but it still works.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: While We are Talking SABs... [Re: wtfcrazymofo]
#23589189 - 08/29/16 07:00 AM (7 years, 4 months ago) |
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Here is where I think I stand on UV in SABs. I can get at least 99% success in a regular SAB. How much can I expect the addition of a UV light to improve my grow?
Most people who are good with agar and work at their procedure don't see contams because of poor work practice. It's usually some other vector like bad filters or high CFU in grain or insufficient sterilization cycles. They rarely end up with bad inoculate because they can see if the inoculate is clean. Cause agar.
The UV sounds fun. But it's like putting spinners on a work truck. In the end all it does is look different. OP your truck looks good, you don't need the spinners.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Hey p.e, my point, is that contamination happens regardless of the type of setup one uses (you proved it by answering in the affirmative). I have relatively few contams as I practice aseptic technique. When it comes down to it some people are better at things than others. Also, did you read the part where I said a buddy built it?
And not sure what t.c.'s definition of success is but you can check some of my oyster grows on the GMM board. I think we're all here to learn and it's a disservice when people state opinion as fact...
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: While We are Talking SABs... [Re: Mycolorado]
#23589838 - 08/29/16 12:16 PM (7 years, 4 months ago) |
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As I recall only facts were stated as facts..
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: While We are Talking SABs... [Re: Inocuole]
#23590068 - 08/29/16 01:35 PM (7 years, 4 months ago) |
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Oysters are even easier than cubes until fruiting
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: While We are Talking SABs... [Re: Pastywhyte]
#23590399 - 08/29/16 03:18 PM (7 years, 4 months ago) |
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Quote:
wtfcrazymofo said: \Do you run the uvc when you are doing your work? If so do you need safety glasses? Your sab is a Caddy. If you want comfortably flow hoods are where its at bro. I'm 1 $250 hepa filter away. My sab is as ghetto as it gets, but it still works.
no i just have bright LED lights in there, and a crayola tracing pad i sometimes use to backlight the agar during transfers (same one i use for taking pics). I was just throwing that UV-C thought out there for talking points, wanted to hear some feedback
but to answer your question: no, the way i am talking about would not use the UV-C while working. I am talking about installing a UV-C light into the SAB with a remote switch, and running the UV-C for a short cycle while it is closed up in the closet (no UV-C light on my skin or eyes at any point), right after i wet the walls. Then load it up and proceed as usual. I suppose you could have uninoculated stuff like jars/plates in there already when you run it, but that is not what i had in mind. what i had in mind is just running a short cycle in the empty SAB shut in the closet, then turn it off, turn on the LED, load it up, and get to work
Quote:
Pastywhyte said: Here is where I think I stand on UV in SABs. I can get at least 99% success in a regular SAB. How much can I expect the addition of a UV light to improve my grow?
Most people who are good with agar and work at their procedure don't see contams because of poor work practice. It's usually some other vector like bad filters or high CFU in grain or insufficient sterilization cycles. They rarely end up with bad inoculate because they can see if the inoculate is clean. Cause agar.
The UV sounds fun. But it's like putting spinners on a work truck. In the end all it does is look different. OP your truck looks good, you don't need the spinners.
NIGGAZ BE TRIPPIN ON MY SPINNAZ!!
lol makes perfect sense pasty, well said. thats pretty much what i figured to begin with, i never had any actual plan to install a UV-C, was mainly just interested in hearing what yall had to say about it in the context of a SAB that is shut in a closet , as talking points
thanks for all the info and feedback everyone
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: While We are Talking SABs... [Re: bodhisatta]
#23590503 - 08/29/16 03:53 PM (7 years, 4 months ago) |
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Quote:
Trusted Cultivator said: Oysters are even easier than cubes until fruiting
Ha, at least you're predictable.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: While We are Talking SABs... [Re: c10h12n2o]
#23590530 - 08/29/16 04:00 PM (7 years, 4 months ago) |
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I think the U.V. is a great idea if you can afford it. At some point I'd like to sell edibles to markets or superstores and I'd like the best equipment I could get. I love tools, and could easily spend all my money on them just to have to nicest things possible. I use tools every day for work and I've noticed the better stuff I have, the easier my job is. Hell, people waste money on far worse things.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: While We are Talking SABs... [Re: Kenetic]
#23590831 - 08/29/16 05:12 PM (7 years, 4 months ago) |
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me for some great offers on mold repellent crystals
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: While We are Talking SABs... [Re: spacechildo]
#23591063 - 08/29/16 06:13 PM (7 years, 4 months ago) |
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ha ha I see what you did there....
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: While We are Talking SABs... [Re: Kenetic] 1
#23591265 - 08/29/16 07:05 PM (7 years, 4 months ago) |
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Quote:
kenetic said: I think the U.V. is a great idea if you can afford it. At some point I'd like to sell edibles to markets or superstores and I'd like the best equipment I could get. I love tools, and could easily spend all my money on them just to have to nicest things possible. I use tools every day for work and I've noticed the better stuff I have, the easier my job is. Hell, people waste money on far worse things.
i totally agree with the jist of what you are saying: i LOVE lab equipment and medical equipment and have good relationships with several local companies supplying both, and i constantly research better ways to do things, mainly trying to learn from the way things are done in an academic setting, and by other people who are truly skilled in these practices and i try to imitate them and learn the underlying principles. i have collected nice lab and medical equipment since i was a kid. and it can really broaden your horizons and save lots of time if you find/use the right stuff
BUT what is WAAAAAAY more important than having the right tool is using the tool RIGHT, understanding the purpose of the tool and what you are trying to accomplish, and utilizing it properly.
For instance, there are some folks on here who have built or bought badass flow hoods and still cant get clean cultures, and then there are people who can work in an SAB they made stone drunk with less than 1% contam rate. So which tool is better? its kind of relative (and i dont like thinking in terms of superlatives anyway, its a fast track to being wrong; something is only "best" until someone thinks of something better)
I have started asking the old men at the lab supply stores how they would go about doing various things, just about anything i try to do. they are brilliant, and often there is a WAY simpler and more effective way to do things than my initial assumptions. Discovering buchner funnels and vacuum aspirators changed my life!!! has probably saved me hundreds of hours
another time, i was doing this crazy extraction the most roundabout way possible, and i asked my 74 yr old buddy up there how to get some crazy chem, he asked what i was trying to do, i explained, and he said "well that would work if you have time and money to burn, but why dont you just use a cation resin in a chromatography column?" and named some particular resin that worked perfect for what i was tryin to do!! saved me hundreds of hours. the old folks are sharp, definitely worth pickin their brains
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: While We are Talking SABs... [Re: c10h12n2o]
#23591931 - 08/29/16 10:31 PM (7 years, 4 months ago) |
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Lol if you have a real chromotogography column you got one helluva lab
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: While We are Talking SABs... [Re: spore-ty] 1
#23593851 - 08/30/16 02:05 PM (7 years, 4 months ago) |
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hahaha maybe its a decent lab for someone who's degrees are in english, but its very amateur to someone who has a few organic chem classes under their belt. but i am learning! ive always collected stuff like that, i love learning how things work and love breaking things down
after i got in good with some lab supply companies, i would go spend a large chunk of my check with them every time i got paid. i love having the ability to do acid/base extractions and other random stuff without getting too ghetto, and it is also great to be able to experiment with various solventless extraction methods for the cannabis industry, being able to test stuff small scale allows me to plan larger and connect the dots.
between my lab stuff and 3d printing i can prototype and test stuff more easily than i ever imagined would be possible, since i grew up in the middle of nowhere in the deep south, where i struggled to find basic supplies, much less lab equipment
also handy to be able to do reagent tests and do broad ID, something i help friends with anytime they think they are buying a particular powder that might test as another compound entirely, though i havent done much of that for several years. (if anyone is interested in doing this on the go, check out Dance Safe kits, you can order one for less than 50$ that will have 3 or 4 different reagent test kits in it and a chart showing the reactions of different substances to each reagent)
lol i will never forget one time in college a guy swore he had pure cocaine, and nothing i could say would change his mind. he had watched blow and thought melting point is the ultimate test of purity. i already knew it was gonna be more like 30% from sampling it (still high for USA), but this guy loved talking about how pure it was. i finally convinced him to wash it and find out and he literally cried when he saw how little was left hahaha
btw chromatography columns themselves arent that expensive, you can get a small one under 100$, but all the stuff you need to use it can definitely add up fast... i always have a list of stuff i am looking for, with craigslist and ebay alerts set up for all of em, and i try to prioritize the stuff i "need" by how many different things i can use it for, and how much it will expand my capabilities
though my own limited understanding and knowledge are usually the primary ceiling i run into. i try not to buy too much stuff i dont understand how to use. and quite often, simply reading more, learning more, or asking the right person the right question is the best way to solve a problem.
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: While We are Talking SABs... [Re: c10h12n2o]
#24374912 - 06/03/17 08:59 PM (6 years, 7 months ago) |
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sorry for the old bump. but what is the material you are placing on the hole openings? pretty cool. i use magnets myself but i like the way that looks. i used to use those nice big pvc pipe fitting but a few times i dropped my sab they came off and i got too lazy to glue em back on.
check out my old one.
 
and the new one.
 
i definitely like my ac magnets better than the old pvc pipe design. the larger pvc would reduce the space in my sab as well.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: While We are Talking SABs... [Re: eatyualive]
#24375229 - 06/03/17 11:49 PM (6 years, 7 months ago) |
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no worries
i was using little plastic pails from the hardware store, just stuffing them in the holes to block them. i usually had them turned the other way around, but i was still figuring that out when i took that shot lol...
i would have put magnets on the side of the pails with a little bar for it to snap onto if i still had this SAB, so that the pails snap into place with the magnets (i really like that idea)
yours is slick! i dig it i tried pipe fittings on my first one but my arms are too big lol... i couldnt find any big enough to get in so i ended up cutting off the bottom of pails and putting a shower cap on it when not in use
i REALLY enjoyed working with that thing, i built it out of plexiglass i found on the side of the road lol... it had a hinged lid where i could put bags in and out, and when i started using a towel and rack combo my success rate went over 99% (for real, i actually tracked it, i did over 15,000 plates in this so i had a plenty big enough sample size lol )
couldnt be happier with my new FH though, i LOVE this thing, i havent even seen contam since i built it (except on a plate i dropped on the floor lol)

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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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