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Invisiblec10h12n2o
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While We are Talking SABs...
    #23557396 - 08/19/16 04:21 PM (7 years, 8 months ago)

I've noticed quite a bit of SAB / GB discussion of late, so i figured i would share some of mine, for the benefit of anyone trying to do the same.

Also, I would very much appreciate any feedback/advice/violent hate from you vets out there :wink:

I recently got back into cultivation and wanted to do it right this time, using what i have learned over the years and approaching it seriously. Since I was to be doing lots of agar work this time around, i wanted a good glove box. After a bit of digging, i found this killer link for a great double hatch build: nice glove box plans

I made several modifications to this plan, especially since my arms are too big to fit into ANY of the PVC pipes i can find in stores, and the force of putting on gloves was too much for the plastic, and i added weather stripping and a nonstick mat:


This setup has served me surprisingly well. I have had virtually no contamination in this thing, only an occasional speck in an agar plate (small enough that i would have overlooked it in years past), and never anything that isnt easily avoided during transfers, if ever i chose one of those plates for transfers.

My main issue is i get VERY hot and sweaty when doing a few sleeves of plates, uncomfortably so. This has made me lust for the cool breeze of a flow hood in my face, as has the desire to take better photos of agar plates.

I am rounding up parts for a flow hood, but in the meantime my gf wants all this shit out of the kitchen!! So in rearranging my grow space, noticed a little closet in a room with hardwood floors, and i started to visualize a tailor-made SAB that is out of the way and has the benefit of 3 walls. While i am thinking this over, i drive past a HUGE sheet of broken plexiglass someone was throwing away, so i busted a u-turn and begun planning the box on SketchUp (which i am not good at)


I wanted to build it in a way that the inside surfaces were as flat as possible (rather than having the supports on the inside), to make it easy to clean/wet the sides before use. I also wanted to use mold resistant paint and have a large door or hatch for this purpose, and for transporting large things in and out. I also sealed it with mold resistant caulk and used rubber gaskets to keep from further damaging the free plexiglass. if/when it fails i will upgrade to glass or real plexiglass.

 

 

I just finished it up and am about to put it to the test :grin:

I am considering adding a UV-C fixture behind the LED , and using a remote to turn it on and off. That way, when i want to use the box, i can close the closet tight, turn on the UV-C light for 10-15 minutes, turn it off, then get to work :smile: . What do yall think about this idea? obviously i wouldn't depend on it, as clean technique the only safe bet,  just an extra precaution for 15$. And i know SAB functions based on still air, not sterility; i have had great success with my last SAB doing agar work, and welcome even a slight improvement

Any feedback is much appreciated


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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InvisiblebodhisattaMDiscordReddit
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Re: While We are Talking SABs... [Re: c10h12n2o]
    #23557424 - 08/19/16 04:29 PM (7 years, 8 months ago)

Uv-c will just make your plastic brittle and not as clear. Don't rely on stupid crutches the box does a 100x better job than the light will to keep your work clean if you have the skills to go along with.

I make my arm holes big enough to just move jars and shit in through those holes.

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Offlinemrmazdarx9
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Re: While We are Talking SABs... [Re: bodhisatta]
    #23557440 - 08/19/16 04:35 PM (7 years, 8 months ago)

Quote:

Trusted Cultivator said:
I make my arm holes big enough to just move jars and shit in through those holes.



I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes :rofl:
Although looking at my last plates you would be forgiven for thinking that I hadn't shit on the plates they were that bad :whip:


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InvisibleInocuole
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Re: While We are Talking SABs... [Re: mrmazdarx9]
    #23557513 - 08/19/16 05:01 PM (7 years, 8 months ago)

Quote:

mrmazdarx9 said:
I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes :rofl:





:rofl:  Fuck, that was pretty funny.


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Offlinemrmazdarx9
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Re: While We are Talking SABs... [Re: Inocuole]
    #23557523 - 08/19/16 05:04 PM (7 years, 8 months ago)

Quote:

Inocuole said:
Quote:

mrmazdarx9 said:
I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes :rofl:





:rofl:  Fuck, that was pretty funny.



Was an easy one tbh lol


--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN

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Invisiblec10h12n2o
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Re: While We are Talking SABs... [Re: mrmazdarx9]
    #23557539 - 08/19/16 05:07 PM (7 years, 8 months ago)

Quote:

Trusted Cultivator said:
Don't rely on stupid crutches the box does a 100x better job than the light will to keep your work clean if you have the skills to go along with.





.... thats why i said
Quote:

obviously i wouldn't depend on it, as clean technique the only safe bet,  just an extra precaution for 15$




no "depending on crutches" involved, except maybe your reading comprehension.

the two certainly arent mutually exclusive. I have had great results for years by using good clean technique and still air. I dont think addint a 10 second UV-C cycle is going to nullify proper still air or proper clean tech, certainly not a crutch, if anything it might help, thats why wanted to discuss it.

would plexiglass be degraded that quickly? of course i would expect some major damage to paint and plastic if it was run 24/7, but 10 minutes every 2-3 days?

Quote:

mrmazdarx9 said:
Quote:

Trusted Cultivator said:
I make my arm holes big enough to just move jars and shit in through those holes.



I'm not great at sterile techniques just yet but damn it I've been skipping the shitting through the arm holes :rofl:




lol


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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InvisiblebodhisattaMDiscordReddit
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Re: While We are Talking SABs... [Re: bodhisatta]
    #23557553 - 08/19/16 05:10 PM (7 years, 8 months ago)

If I ever build another SAB I won't have arm holes just one big ass oval in the front


Also I read your post just fine, even that next sentence... I could care less. If you say uv-c you get my uv-c standard opinion. Which you dont have to listen to, especially not get asshurt about either.

Most real labs have moved away from using UV-C stages on their hoods I can only imagine the reasoning is because it's a gimmick in that particular application

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Invisiblec10h12n2o
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Re: While We are Talking SABs... [Re: bodhisatta]
    #23557587 - 08/19/16 05:20 PM (7 years, 8 months ago)

your standard uvc treatment is usually in response to someone trying to pasteurize or sterilize bulk substrate, or grains, or something else that would be trying to rely on it instead of proper tech. Clearly this is a different context

no anus injuries here, you just take things out of context quite often and i needed to call you out about it to keep the conversation on point, and distinguish it from your "standard opinion" about another use in another context


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Edited by c10h12n2o (08/19/16 05:21 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: While We are Talking SABs... [Re: c10h12n2o]
    #23557590 - 08/19/16 05:22 PM (7 years, 8 months ago)

People like to avoid the advice they know they are going to get by adding the

"I know it's unnecessary/overkill/dumb "

Only makes me more eager to post the thing that's going to make you say that I don't know how to read

:shrug:

I'm not the one asking about bullshit

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Invisiblec10h12n2o
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Re: While We are Talking SABs... [Re: bodhisatta] * 1
    #23557688 - 08/19/16 05:49 PM (7 years, 8 months ago)

because nothing screams lack of bullshit like someone christening themselves with a DIY Trusted Cultivator tag :rofl:

anyways, any actual discussion about using a supplemental uv-c cycle in this type of SAB is much welcome. as a supplement to proper sterilization/pasteurization & clean tech, NOT as a substitute for any of these. some of us actually enjoy thinking about these things, irrespective of any kind of endorsement


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Edited by c10h12n2o (08/19/16 05:54 PM)

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Invisibledankington
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Re: While We are Talking SABs... [Re: c10h12n2o]
    #23557724 - 08/19/16 05:59 PM (7 years, 8 months ago)

Oh sure, sure! Snub the man that spends 50% of his time at work in a laboratory.

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InvisiblebodhisattaMDiscordReddit
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Re: While We are Talking SABs... [Re: c10h12n2o] * 1
    #23557745 - 08/19/16 06:06 PM (7 years, 8 months ago)

sorry I took the most obviously awesome username available hah

so you'll try UVGI in your SAB. you'll load up all your jars, and dishes etc... then you'll turn the lamp on for 10-15m but there's the line of sight problem. so you'll have to go and rotate your shit around a few times or just the one side gets exposed.

you won't want to put in any of your spores petris, or inoculum in there because you don't want to damage it's DNA before using it.

then you'll turn it off (or forget to and get some sunburn, yes I know it's longer UV wavelengths that cause that in humans, but that's because the short ones don't make it down to the surface of the planet) the short wave UVC is actually worse for the skin and causes sunburn faster.

then with the lamp off you have to put your inoculant, dishes, spores back into the SAB which effectively will undo the cleaning the UVC did

you'll still have to wipe down everything in alcohol which is just a sanitizer anyway. bringing you back exactly to square one before you turned the lamp on.

you'll spray the SAB down before using it, but you'll have to remember to clean your bulb every time since residue, film, hard water deposits, dust, etc... greatly diminish the effectiveness of the bulb

then there's the degradation of your plastics including the SAB and anything else UV sensitive.

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InvisibleInocuole
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Re: While We are Talking SABs... [Re: bodhisatta]
    #23557750 - 08/19/16 06:08 PM (7 years, 8 months ago)

There are way shittier cultivators on this site with real tags.


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InvisiblebodhisattaMDiscordReddit
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Re: While We are Talking SABs... [Re: Inocuole]
    #23557758 - 08/19/16 06:12 PM (7 years, 8 months ago)

Quote:

Inocuole said:
There are way shittier cultivators on this site with real tags.



well to be fair some of them are not active anymore,


some of them are though

blackout
CH HELL
eatyualive
george castanza
hamloaf
tahoe
_Otto_

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InvisibleInocuole
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Re: While We are Talking SABs... [Re: bodhisatta] * 1
    #23557763 - 08/19/16 06:13 PM (7 years, 8 months ago)

I see 3-4 mediocre cultivators and then a few decent cultivators that you don't like. :lol:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisiblebodhisattaMDiscordReddit
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Re: While We are Talking SABs... [Re: Inocuole]
    #23557792 - 08/19/16 06:20 PM (7 years, 8 months ago)

those other 3-4 are plenty fine at growing mushrooms but their advice is garbage

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InvisiblebodhisattaMDiscordReddit
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Re: While We are Talking SABs... [Re: bodhisatta]
    #23557795 - 08/19/16 06:21 PM (7 years, 8 months ago)

one particular person that reminds me of the days in the year almost made that list for a shumvbox

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Re: While We are Talking SABs... [Re: c10h12n2o]
    #23557926 - 08/19/16 06:59 PM (7 years, 8 months ago)

dear OP,

there is no need to wrap your minitubs with syran wrap. in fact, I would advise against that.

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OfflinePussyFart
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Re: While We are Talking SABs... [Re: bodhisatta] * 1
    #23557936 - 08/19/16 07:03 PM (7 years, 8 months ago)

Quote:

Trusted Cultivator said:
one particular person that reminds me of the days in the year almost made that list for a shumvbox



:lmafo:

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Invisiblec10h12n2o
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Re: While We are Talking SABs... [Re: Munchauzen]
    #23557959 - 08/19/16 07:12 PM (7 years, 8 months ago)

good info bodhi, though its a little different than what i had in mind. and also, i have been having the same level of success just using soap and water (as per RR suggestions) as i did with bleach and/or iso. what i had in mind was to soap/wet the sides and then run it 10 minutes in the empty box (shut in the closet, using a remote), and then proceed as normal. i dont see how it would be any more trouble than usual when used in this way, and searching academic databases there are dozens of examples of uv-c light being used in exactly this way. anyways, it was just a thought, certainly not some "tek" i advocate, just talking points

lol and not snubbing anyone, i was just pointing out that the question was certainly not bullshit. context is very important... and bodhi isnt the only person who spends at least 50% of their time in a lab, thats part of what makes this place so great :smile: but correct ideas are what matters, not ego or personalities

and i totally agree inocuole, without a doubt :laugh:

Re Munch: those are actually not mini tubs, they are trays that are colonizing. but I very much appreciate the advice!! does that change anything? i have 5 holes punched in the wrap with micropore tape over each. much obliged my friends

Edited by c10h12n2o (08/19/16 07:23 PM)

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