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Shrooms4fun
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Registered: 11/27/15
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Last seen: 6 years, 11 months
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First Time Attempt
#23556704 - 08/19/16 12:29 PM (7 years, 5 months ago) |
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So after doing a lot of research and slowly gathering materials i am about to attempt my first grow. I have compiled and gathered a lot of different information and have put together what i feel will be an easy and efficient way to grow in bulk im just posting here for feed back as well as other thoughts and ideas.
First off i will be using the BRF Tek as it seems to be the simplest route to go and does not require the use of a pressure cooker the instructions i will be following for the cakes and prep will be the information i have gathered from this youtube video:
as far as that series go's the only thing im really taking from it is the cake preparation and im also debating on adding (if i recall correctly) 1tsp of gypsum per cake into the mix as well.
as far as lids are concerned i will be using metal lids pretty simple as they came with my half pint jars i will be making my own filters with a tek i found here on the forum using 1/2 inch sheet polyfill and an iron apparently these filters are the equivalent to an SFD. Resources for this information was found here:
https://www.shroomery.org/forums/showflat.php/Number/19938845/fpart/1/vc/1
i also intend on using a Tote-in-Tote incubator to speed up the mycelium growth following a tek written by frank located here:
https://www.shroomery.org/forums/showflat.php/Number/17954773/fpart/1/vc/1
Substrates im not so sure about yet or what exactly im going to do or in what measurements i do have a lot of the basic things to create a substrate for my monotub. Gypsum, coir (actually i have 1 giant brick which was a bad idea it will mess with my measurement accuracy), dried horse manure, perlite, and vermiculite. I ned to figure out how to deal with that large brick if coir i bought off amazon...i think its more to expand than i can do in a 5 gallon bucket. Im also not sure exactly how i would like to pasteurize anything yet i kinda like franks methos in the quart jars ( look here: https://www.shroomery.org/forums/showflat.php/Number/17246844#17246844 ) but im not exactly sure if i will get the correct field capacity and all of that correct using that method.
The Coir i bought: https://www.amazon.com/Roots-Organics-Compressed-Fiber-Fertilizer/dp/B00IGFGNMU/ref=pd_nav_hcs_bia_t_1?ie=UTF8&psc=1&refRID=SQJC8D3YA268JYS99G2D
now for the monotub part of the project i am combining 2 teks that are very informative, one is absolutly great for the actual construction and fits nicely with the ironed out polyfill tek and the other has great information on how to prepare and dial in my mono tub and what to look for and all of those great things
construction of monotub: https://www.shroomery.org/forums/showflat.php/Number/20115107
how to dial it in and what to watch for: https://www.shroomery.org/forums/showflat.php/Number/17332777#17332777
what i really need to figure out for my monotub is a decent way to make some sort of permanent liner to pull the substrate out as a whole for harvest, the thought has crossed my mind to just buy another tub the same size as my monotub and cut the bottom 4 inches off or so and use that as a liner but that feels like such a waste and the tubs are not exactly cheap.
i think as far as supplies all i really need to get around to make everything work as i would like is a fish tank air pump 2 more tubs (one to make a SOB with and one to build a monotub with) and that should be about it for the bare minimum, as far as strain choice i chose to grow Cambodian as i have herd from somewhere (i dont remember the exact souce honestly) that this is a noob friendly strain.
My work area is going to be my damp and nasty basement ive got leaky water pipes and all that great crap down there it even smells musty but its the only spot i have to work with, i have how ever put together a plan to clean it all up and sanitize it spray everything down walls and all with some industrial chlorine i happen to come across to kill any mildew/mold/fungal contaminants floating around down there.
station attire i have gotten some non breathing thin Bio suits non-latex unsterilized gloves full face-shield respirators 70% iso TONS of lysol 91% iso (will use this for the burner as i have read the higher percent does not mean more sanitary/sterile).
thoughts and plans for the near future i would like to play around with liquid cultures i have read a lot about them and they seem to be a good way to create a good infinite supply of mycelium to inoculate sterilized jars with. the teks i have been reading for the LC process are as follows:
https://www.shroomery.org/forums/showflat.php/Number/12830423/fpart/1/vc/1
and
https://www.shroomery.org/forums/showflat.php/Number/7990164
my end goal is to prolong the purchase of an expensive pressure cooker as long as possible for the time being as i do not own or have access to one at this point in time.
any how that pretty much concludes everything i have gathered over the last few weeks and how i intend on going about things if anyone has any feed back thoughts or ideas and im even good with constructive criticism if you wish to apply it i would love to hear from you. if you feel i have left out a detail or some part of information that is not exactly clear feel free to ask it may be something i didnt think about even.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Skip the tub in a tub incubator
No airpumps
Your monotub liner is a trash bag
Buy a pressure cooker skip the bullshit you're adding on to good TEKs so they will actually work
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Shrooms4fun
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Registered: 11/27/15
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Quote:
Trusted Cultivator said: Skip the tub in a tub incubator
No airpumps
the TiT thing is something i have read a lot of mixed feelings about some people say use them to reduce time (a lot of the BRF teks i have read actually say to use them) others say they cause issues. The air pump is for the TiT to circulate the water and maintain an even temprature.
Quote:
Your monotub liner is a trash bag
does it all remove in a pretty solid piece with just a trashbag? i would think it would remove rather flimsily and cause the substrate to break apart or something to that effect
Quote:
Buy a pressure cooker skip the bullshit
affordability is the primary issue here i would like to buy one eventually but would like to start this without one for the time being not having one limits me too much to following particular methods but at this point in time it really is not feasable to drop $70 on one at walmart
Quote:
you're adding on to good TEKs so they will actually work
not sure i know what you mean here. im taking parts of teks that i like or am capable of doing or getting materials for and just going with a plan that should all be uniform and essentially all of the processes are the same but the construction of some parts i just like better than the others like Franks TiT or the way that video sterilized the BRF jars with no PC.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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The only thing more useless than an incubator is a hygrometer
If your temperature is above 65 you're fine
Trash bags totally don't work I was just lying about that.........
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dankington
The Stranger




Registered: 03/14/15
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Loc: 8te
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trash bags are fine for liners. I just leave them flat, and cut them to size.
Pick 1 tek first. They aren't like legos, don't mix and match. No PC? You're basically relegated to pf tek.
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Shrooms4fun
Stranger
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Last seen: 6 years, 11 months
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Quote:
dankington said: Pick 1 tek first. They aren't like legos, don't mix and match.
well is there a specific tek someplace that describes going from BRF cake to a monotub tho? i dont feel that fruiting and such (shown in the other parts of that youtube video)should be done souley on the cake itself and a perlite bed.
like i stated before i only used parts of other teks for things like constructing jar lids and things of that nature.
this guide here describes just about everything i want to do aside from the monotub for colonizing and fruiting:
http://www.fungifun.org/English/Pftek#a1
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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I always say fruit cakes as cakes. It's not nearly worth the hassle to use them as spawn for a bulk grow other than experience.
If you want a mono spend they money you would have on cake shit, (plus what you spent on incubators, heating pads, humidifiers, air pumps) and buy two pressure cookers
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Shrooms4fun
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Quote:
Trusted Cultivator said: I always say fruit cakes as cakes. It's not nearly worth the hassle to use them as spawn for a bulk grow other than experience.
experiance is my key factor tho and the fact i have zero experience makes me want to take the simpler route }(the PF or BRF tek) while trying to go bulk at the same time....i dont understand why once i have obtained spawn mycelium why it would not spread to the monotote environment i would like to understand exactly why this perception would not work in my favor or why you find it as a bad idea in detail.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Quote:
korn16ftl3 said:
Quote:
Trusted Cultivator said: I always say fruit cakes as cakes. It's not nearly worth the hassle to use them as spawn for a bulk grow other than experience.
experiance is my key factor tho and the fact i have zero experience makes me want to take the simpler route }(the PF or BRF tek) while trying to go bulk at the same time....i dont understand why once i have obtained spawn mycelium why it would not spread to the monotote environment i would like to understand exactly why this perception would not work in my favor or why you find it as a bad idea in detail.
so it will take 20+ cakes to make a full monotub. given you are starting from spores, you have almost a 100% gaurentee that one of those 20 some cakes will be contaminated and ruin the entire fucking monotub. the only way to do cakes->monotub is to inoculate the cakes with a clean liquid agar solution, not spores. spores are never clean, and pf-tek cakes are the only viable method to grow from spores.
fruit them as cakes via the PF Tek if you actually want mushrooms and not failure aka 'experience'
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busutdori
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If you spend some time on the cultivation forum, you'll notice that 99% of TiT and air pumps are heavily frowned upon. Been lurking on shroomery for about 6 months and I can recall maybe one post where TiT actually worked. If you've done your research then I'd say you were reading very outdated info.
Also, pressure cookers are not $70...more like $40 and half that if you get one second hand.Pressure cooker and a SAB are the two most versatile and essential tools you'll buy in this hobby imo. Lets you try out basic teks like PF tek and also advanced work with isolating strains on agar. I say that a pressure cooker is a worthwhile investment if you want to keep up with this hobby.
Edited by busutdori (08/19/16 10:05 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: First Time Attempt [Re: busutdori]
#23558510 - 08/19/16 10:08 PM (7 years, 5 months ago) |
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Boogieman47
Let's boogie


Registered: 03/05/16
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Amazon is probably the cheapest at 79$ for a 23 qt and good luck finding a used one unless you're into 6-10 qt ones
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Shrooms4fun
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Quote:
Munchauzen said:
Quote:
busutdori said: Also, pressure cookers are not $70...more like $40
used maybe
https://www.walmart.com/ip/Presto-23-Qt-Pressure-Canner/2625289 https://www.amazon.com/Presto-01781-23-Quart-Pressure-Canner/dp/B0000BYCFU
not much reason to buy anything less than a 23 quart
the one locally here in town was about $60 but i dont think it reached the 15 PSI called for in a lot of the procedures here and i think its a bit smaller than 23QT. its canning season around here and this stuff is set out at our local retailers.
from what i have gathered a second hand pressure cooker isent always a good idea i did see one at a yard sale but it was missing the steam rocker and they wanted like $20 for it so i passed on that.
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Kjay123
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All I hear is spores are no good for monotubs, or grain substrates , I did two and 12 regular pf cakes , my first grow , my monotub is on its second flush now , I buried one bag out back and got a huge 245g wet flush , 10 of cakes worked fine , 4 took an extra week but are just now starting to fruit , I understand the benefits of isolation and agar , but giving newbies bullshit info is not cool "experts"
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: First Time Attempt [Re: Kjay123]
#23559668 - 08/20/16 11:02 AM (7 years, 5 months ago) |
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dankington
The Stranger




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Re: First Time Attempt [Re: Kjay123]
#23559674 - 08/20/16 11:05 AM (7 years, 5 months ago) |
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a "huge" 245g wet flush from a bag isn't amazing at all. In fact, I typically get 245+g wet from a quart of spawn in a mini mono. we simply like to try and optimize results, so… no need to get your panties in an uproar.
Spores directly to grain is just so dicey. That's why we recommend people use either agar or pf cakes.
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bodhisatta 
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Registered: 04/30/13
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Loc: Milky way
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Two quarts MS AA+ on oats Two quarts MS ESS on oats 54quart sterlite locking lid tub One brick coir + quart vermiculite No gypsum
First flush dry 5.5 oz

If spores don't work don't blame the spores. There's enough genetics in even a limited MS grow (spores diluted before inoculation or a couple agar transfers IE less strains) that you can push it to perform if given adequate conditions (clean spawn, proper moisture and moisture maintenance of needed, and fruiting conditions)
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Kjay123
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I got a massive cluster , all connected to one or two main stems , weighing in at 245gs, still getting fruits every morning from it , so I am not sure if it's on its last leg, or first flush still
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Kjay123
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Re: First Time Attempt [Re: Kjay123]
#23559987 - 08/20/16 01:33 PM (7 years, 5 months ago) |
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My total dry weight on my first grow right now is at 2.5 oz, all still going , my monotub is just starting to pin again , Cakes didn't put out too much , but at least they are producing , down to 11 out of 12 jars still going , ALL MS SYRINGES, and my grain bags worked great with multi spore , my first topic , I had multiple "pros" treat me like a dick and tried to convince me all my shit was contaminated, real welcoming place too guys , I wonder how many newbies you guys put off to this hobby , not directed towards everyone , just some of you jerk offs
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: First Time Attempt [Re: Kjay123]
#23560015 - 08/20/16 01:42 PM (7 years, 5 months ago) |
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No one was trying to treat you like a dick or be dicks well in a good way I guess all the pros or vets or however you wanna call them tell you what works and what doesn't man you may get lucky a few times but what they are trying to do is steer you in the right direction of this hobby if you can't take the criticism don't ask questions or suck it up trust me it's frustrating but I'm sure just like anyone else that starts this they have done some stupid shit which is why they tell you what they tell you don't take it personally
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Shrooms4fun
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As a quick update I have my half pint jars all made up but that making an sfd guide was I'm sure accurate and is completely doable however I need to develop a nack for it so I just ordered some SFD's from one of the vendors at the top of this site. After a little bit of work with the thin metal of the jar lids the larger GA hole proved to be a serious challenge as the drill bit kept shredding the hole as it passed threw the metal.....so in hind sight I think it would be a good idea to invest in a punch that is capable of punching holes in thin metals.
Edited by Shrooms4fun (08/27/16 08:52 PM)
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Tuhdoww
Sub Slapper


Registered: 08/23/16
Posts: 300
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Quote:
Trusted Cultivator said: Two quarts MS AA+ on oats Two quarts MS ESS on oats 54quart sterlite locking lid tub One brick coir + quart vermiculite No gypsum
First flush dry 5.5 oz

If spores don't work don't blame the spores. There's enough genetics in even a limited MS grow (spores diluted before inoculation or a couple agar transfers IE less strains) that you can push it to perform if given adequate conditions (clean spawn, proper moisture and moisture maintenance of needed, and fruiting conditions)
That made for a nice pic. That's cool dude!
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Shrooms4fun
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Re: First Time Attempt [Re: Tuhdoww]
#23657262 - 09/18/16 10:44 PM (7 years, 4 months ago) |
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So I now have 36 half pin jars made and ready for substrate and inoculation.
A few quick questions before I get as far as steam sterilization,
1) will it hurt anything if I sterilize beyond an hour? For example 1.5 hours?
2) I read a lot of people using oust in both the SAB and in the air around them to kill floating contams in the air, would lysol be a suitable substitute?
3) what is the recommended additive of gypsum to the 5 cake recipe given in the video I linked in the OP?
4) I haven't read or recall hearing in the video a recommended cool down period after steam sterilization. Would it be safe to sterilize all the jars and noc them the next day?
5) when inserting the needle into the injection point I'm assuming the needle is to pass threw the top layer of verm into the actual brf substrate. Am I correct in this assumption as none of the demonstratin videos really show this detail nor specify
Edited by Shrooms4fun (09/18/16 10:51 PM)
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mushboy
modboy



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Loc: where?
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Quote:
Kjay123 said: My total dry weight on my first grow right now is at 2.5 oz, all still going , my monotub is just starting to pin again , Cakes didn't put out too much , but at least they are producing , down to 11 out of 12 jars still going , ALL MS SYRINGES, and my grain bags worked great with multi spore , my first topic , I had multiple "pros" treat me like a dick and tried to convince me all my shit was contaminated, real welcoming place too guys , I wonder how many newbies you guys put off to this hobby , not directed towards everyone , just some of you jerk offs
got pics?
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Shrooms4fun
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Re: First Time Attempt [Re: mushboy]
#23657852 - 09/19/16 05:50 AM (7 years, 4 months ago) |
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Wow so much animosity around here or at least on this thread
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wtfcrazymofo
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1.no 2.no just make sure the sab walls are wet with a soapy water solution and wait for the dust to fall that is in the air for 15 or so in a no draft room. 3. what link? there is no said amount of gypsum just a random ass handful, or like 4-5% 4.yes 5.yes, and do the videos use ships?
seemes to me like you got grain jar lids and not pf tek lids.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Shrooms4fun
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Quote:
wtfcrazymofo said: 1.no 2.no just make sure the sab walls are wet with a soapy water solution and wait for the dust to fall that is in the air for 15 or so in a no draft room. 3. what link? there is no said amount of gypsum just a random ass handful, or like 4-5% 4.yes 5.yes, and do the videos use ships?
seemes to me like you got grain jar lids and not pf tek lids.
3) this video is the 5 cake formula I will be following
5) ships? Don't believe I have herd this term before.
Lids: from my understanding all lids need a port for injection and GE. Will/does the performance of colonization vary from tek yo tek based on lids aline as long as these 2 conditions are met? All guides for lids were pretty simple and basically the same as these aside from the lids in the above linked video which uses 4 holes used as injection ports then later covered with microspore tape (medical tape). Any ideas on how/if the lids I have made will function vs the 4 hole lids used in the video?
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7munkee
Berilion



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Self Healing Injection Ports, or ships. I use high temp silicone and it works well.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 18 minutes
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As long as you can have more than one inoculation point (4) it should work. The top layer of dry verm is for a contam barrier so with your whatman lids you wont really need it, but you should do it anyway.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 18 minutes
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Quote:
korn16ftl3 said: As a quick update I have my half pint jars all made up but that making an sfd guide was I'm sure accurate and is completely doable however I need to develop a nack for it so I just ordered some SFD's from one of the vendors at the top of this site. After a little bit of work with the thin metal of the jar lids the larger GA hole proved to be a serious challenge as the drill bit kept shredding the hole as it passed threw the metal.....so in hind sight I think it would be a good idea to invest in a punch that is capable of punching holes in thin metals.

Yeah I think thsoe lids suck for this tek. you wont be able to get the spores all around the instde of the jar. Just use the lids in the video. It has been done 1000s of times with metal lids upsidedown with 4 holes.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Shrooms4fun
Stranger

Registered: 11/27/15
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Last seen: 6 years, 11 months
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Quote:
korn16ftl3 said:
Quote:
wtfcrazymofo said: 1.no 2.no just make sure the sab walls are wet with a soapy water solution and wait for the dust to fall that is in the air for 15 or so in a no draft room. 3. what link? there is no said amount of gypsum just a random ass handful, or like 4-5% 4.yes 5.yes, and do the videos use ships?
seemes to me like you got grain jar lids and not pf tek lids.
3) this video is the 5 cake formula I will be following
5) ships? Don't believe I have herd this term before.
Lids: from my understanding all lids need a port for injection and GE. Will/does the performance of colonization vary from tek yo tek based on lids aline as long as these 2 conditions are met? All guides for lids were pretty simple and basically the same as these aside from the lids in the above linked video which uses 4 holes used as injection ports then later covered with microspore tape (medical tape). Any ideas on how/if the lids I have made will function vs the 4 hole lids used in the video?
Screw it I just made standard pf tek lids with the 4 homes anyone e else have any other input from up above there?
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Shrooms4fun
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Here is a thought how about I use an under the bed sterilite tote like the one in the picture I'm going to upload to store all the pf jars in? I mean would this restrict airflow too much? Should I drill some half inch holes threw the sides or something? This is just a thought for easy storage and mobility of 36 pf jars feedback would be appreciated :-) I have pets and hard floors so dirt getting kicked around is pretty common
Edited by Shrooms4fun (09/20/16 09:03 PM)
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dankington
The Stranger




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Looks good dude. And those lids would have been fine for PF tek, as long as you make sure there's GE going on. I'd have pushed in the needle against the glass so I could see the inoculation point, then rolled the jar around a bit to spread the solution. Just a couple drops.
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Shrooms4fun
Stranger

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Quote:
dankington said: Looks good dude. And those lids would have been fine for PF tek, as long as you make sure there's GE going on. I'd have pushed in the needle against the glass so I could see the inoculation point, then rolled the jar around a bit to spread the solution. Just a couple drops.

It was just a few bucks to buy more lids I'm not to concerned about it I'll just use the others if I decide to go the grain route seems if the pf tek is simple the perhaps I should just mass produce the half pint jars and spawn to bulk coir with the 50/50 I've been reading about. At least if I get the pf tek down contam free and the myc is resilient after 100% colonized as well as coir and verm being contam resistant I should be able to do a whole tub with 35 or 36 jars easily from start to finish pretty much contam free I would imagine.
As far as the monotub idea gos I read a lot about people dunking between flushes....I'm assuming that means harvest then remoisten the sub? If that is the case how does one get it back toward the field capacity range?
Edited by Shrooms4fun (09/21/16 07:23 AM)
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dankington
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I hightly recommend fruiting cakes as cakes. You won't notice any appreciable yield increase by spawning the cakes, and cakes are always riddled with contams.
Image courtesy of Adden
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Shrooms4fun
Stranger

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Quote:
dankington said: cakes are always riddled with contams.
I'm curious about this, isent that why we sterilize?
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dankington
The Stranger




Registered: 03/14/15
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It's the nature of the method. Mushrooms don't grow in sterile environments, so their spores aren't either. Spore syringes are as clean as they can get them.
Essentially, BRF cakes are so nutritious, that the myc is often able to perform, despite all the bacteria present. That's why cakes aren't very good for spawn. You want your spawn to be as clean as possible. Minimize all the vectors you can.
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Shrooms4fun
Stranger

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Quote:
wtfcrazymofo said: there is no said amount of gypsum just a random ass handful, or like 4-5%
So as per this site: https://www.shroomery.org/5143/What-recipe-should-can-I-use-for-PF-cakes
Quote:
The basic PF recipe is 1/4 cup brown rice flour (measure the flour itself, not the brown rice if you grind it yourself), 1/2 cup vermiculite(fine is preferred), and 60ml (4 tablespoons) of water per jar.
and according to the recommendations of about 6% gypsum and if my math is correct (someone please correct me if it is because im setting up to do this a soon as i have confirmation).
1/4 cup + 1/2 cup + 4 tablespoons = 1 cup total
6% of 1 cup = 14.1952942 ml
14.1952942 ml = 2.880000933267 tsp
so for 1 cake and the recipe above i would go: 1/4 cup brown rice flour, 1/2 cups vermiculite, 4 tablespoons of water, and 3 tsp of gypsum, per jar.
doing 12 jars this comes out to be:
3 US cups brown rice flour, 6 US cups vermiculite, 48 US tablespoons of water (reduced is 3 cups), 36 tsp gypsum (reduces to 3/4 cups)
this in total should yield me 12 jars of PF cakes. I do not know if i should have included the water in the total volume of the recipe to calculate the % of gypsum as i dont think the water actually adds volume just density.
so does all this seem correct?
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mushboy
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for a batch of 7 quarts i just use too spoonfuls of gypsum..it doesnt have to be exact. your not making a rue.
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Shrooms4fun
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Re: First Time Attempt [Re: mushboy]
#23673782 - 09/24/16 12:00 PM (7 years, 4 months ago) |
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Quote:
mushboy said: for a batch of 7 quarts i just use too spoonfuls of gypsum..it doesnt have to be exact. your not making a rue.
so even a 1/4 cup for 12 pint jars would work?
should i spray the jars out with rubbing alcohol before i use them they are new or will sterilizing take care of all of that for me?
Edited by Shrooms4fun (09/24/16 12:02 PM)
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mushboy
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i know its going to sounds dumb but its really by look/feel for me.
i never used gypsum with pf cakes, only grains. just realized i have no clue if the amount needed effects the pf cake texture one way or the other but 1/4 cup for 12 pints sounds reasonable.
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Shrooms4fun
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Re: First Time Attempt [Re: mushboy]
#23673857 - 09/24/16 12:34 PM (7 years, 4 months ago) |
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all right well i got all the things out and around on the table lets see how this gos i have yet to try this even tho i have 36 1/2 pint jars ready to go rather than waste materials im just going to try in 12 jar increments until i get it down
EDIT: i skipped the gypsum for now and using my calculations i was able to load 17 1/2 pint jars. The reason i have skipped the gypsum for now was because i was concerned about it taking away from the moisture content as it is a dry powder so i figured i would stick to the tek's instructions at this point in time. Perhaps soon i will just do a simple 5 jar grow and try the gypsum and see what happens.
UPDATE: the jars have been steamed and are now cooling might be able to knock them up tonight as i removed them from the pot to cool mostly because i had to run the jars in 2 sets because i could not fit them all in the pot the first time i sterilized
UPDATE 2: Everything is knocked up they cooled from about 630pm until about 10pm and I Knocked them at about 11:30pm lets see how this gos i will keep this thread updated
Edited by Shrooms4fun (09/24/16 10:39 PM)
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Shrooms4fun
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hmmmm how long should this take if going from spores? it has been about 6 days and im not getting any signs of anything yet.
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markisses


Registered: 09/17/06
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Quote:
korn16ftl3 said: hmmmm how long should this take if going from spores? it has been about 6 days and im not getting any signs of anything yet.
Mine took 10 days with 8 month old MS on finely ground brown rice and vermiculite. I believe it could take up to 3 weeks. If it has been longer than 3 weeks, start a fresh batch.
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Shrooms4fun
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Re: First Time Attempt [Re: markisses]
#23705889 - 10/04/16 07:13 AM (7 years, 3 months ago) |
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Quote:
markisses said:
Quote:
korn16ftl3 said: hmmmm how long should this take if going from spores? it has been about 6 days and im not getting any signs of anything yet.
Mine took 10 days with 8 month old MS on finely ground brown rice and vermiculite. I believe it could take up to 3 weeks. If it has been longer than 3 weeks, start a fresh batch.

Well I'm on a week and a half now with no signs of anything so I guess 3 weeks means so far I'm still in the green.....I might buy a premade LC jar from one of the sites vendors and get that started and from there inject the pf jars from the LC myc in order to speed things along a bit, also of this fails I won't waste syringe after syringe.
A note that should probably be made I used normal unsoftened tap water (also note I have well water) in the recipe, would/could this be an issue or cause issues with this project?
Edited by Shrooms4fun (10/04/16 07:17 AM)
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markisses


Registered: 09/17/06
Posts: 68
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I wouldn't worry about speeding up the one's you have knocked up by trying to make some LC to reinoculate. If you do decide to make some LC, just use it on a fresh batch. Remember that using a LC can still allow contaminates to sneak into your substrate. Maybe try your hand at agar to guarantee you're inoculating them with some clean culture.
Checkout Violet's Microwave No-Pour Agar or Pastywhyte's Easy Agar Tek.
I don't believe your well-water would cause any issues. When I had a well back in the day, the water was similar to bottled spring water but it had more minerals in it, which would not affect the mycelium in a bad way. I use bottled spring water on the ones in that pic.
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Shrooms4fun
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Re: First Time Attempt [Re: markisses]
#23709003 - 10/05/16 06:21 AM (7 years, 3 months ago) |
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Quote:
markisses said: I wouldn't worry about speeding up the one's you have knocked up by trying to make some LC to reinoculate. If you do decide to make some LC, just use it on a fresh batch. Remember that using a LC can still allow contaminates to sneak into your substrate. Maybe try your hand at agar to guarantee you're inoculating them with some clean culture.
Checkout Violet's Microwave No-Pour Agar or Pastywhyte's Easy Agar Tek.
I don't believe your well-water would cause any issues. When I had a well back in the day, the water was similar to bottled spring water but it had more minerals in it, which would not affect the mycelium in a bad way. I use bottled spring water on the ones in that pic.
I wasent going to reinoculate the older jars if those dont pan out after 2 months or how ever long I decide to let them set (well past the time they should anyhow) then I'll dump and clean them. I was just going to make an LC to colonize new brf jars quicker. I have looked into ager as well I've seen a few places that sell dishes prepoured in 4 sets or so and have considered goino that route just as an easy way to just check it out right quick. On a plus note I would assume I did sterilization correctly because absolutely nothing has grown as of yet......? Would that be as safe assumption in this case?
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Shrooms4fun
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Well I checked the other 12 jars that I havery been leaving alone (I have a set of 4 of the 16 that I habe been routinely checking)I found 1 jar finally starting to colonize and another contaminated with green mold all others appear to be currently not effected as of now with all jars being inoculated on 9/24 before midnight. So I guess there is still hope for the remaining 14 jars
Look at the white spot a little ways down from the lid it's not real easy to see just yet but this is the good one:

Contaminated jar:
Edited by Shrooms4fun (10/05/16 10:46 PM)
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Shrooms4fun
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another one started colonizing today im starting to get excited now :-)
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Shrooms4fun
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Here is food for thought, So far I only have 2 jars of 18 colonizing (one contamination and 1 I screwed up right from the get go) if these for some reason turn out to be the only 2 cakes that turn out for me would it be possible to transfer from cake to ager and if so where would I do that step in the process? Before or after birthing? This also might seem like a silly question but just for self reassurance after taking let's say 5 ager samples from the cake I can still fruit the cakes after taking the sample correct?
Edited by Shrooms4fun (10/09/16 08:36 AM)
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Shrooms4fun
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*bunp
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amidogen
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Registered: 05/07/16
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
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Re: First Time Attempt [Re: amidogen]
#23724752 - 10/10/16 10:36 AM (7 years, 3 months ago) |
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Ya I'm aware of the cloning option as well how ever I did not think about that when I wrote that post thanks for reminding me....I think the only thing that has me concerned about cloning is how to select a good fruit/parent
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amidogen
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
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Re: First Time Attempt [Re: amidogen]
#23725167 - 10/10/16 12:50 PM (7 years, 3 months ago) |
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That simple to select them eh? Ya I have plenty of spores left....I'm just not sure if I like the syringes....I got them from a vendor and apparently they are shitty vendors from what I have read....so I'm not really sure how this grow is going to turn out. I have 2 LC jars premade from out grow on the way as well so maybe inject like the whole 6 cc of the partial syringe in that? Is there such a thing as over doing an MS injection for an LC?
Edited by Shrooms4fun (10/10/16 06:59 PM)
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amidogen
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Registered: 05/07/16
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
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Re: First Time Attempt [Re: amidogen]
#23726212 - 10/10/16 07:00 PM (7 years, 3 months ago) |
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Quote:
amidogen said: Why do you keep spending $ ordering premade shit? If this site should have taught you anything it's that it's cheaper and more effective to make your own of anything to do with this hobby.
simpler for the time being and i still have yet to order a PC which i really do need to do
Quote:
amidogen said: If you're trying to make liquid anything, you need to use a clean culture to do it or you will waste your time, spores, and sub. The only way to get a clean culture is agar.
By the way, even though we all hate the vendor you got your shit from, you still need to keep vendor names out of cult posts.
Can i just drop a piece of clean culture strait from the ager after a few transfers right into an LC jar with sterile scalpel and an open lid LC jar inside a SAB? From my understanding im not supposed to EVER open the lid of a sterile jar of any sort or as little as possible
Edited by Shrooms4fun (10/10/16 07:12 PM)
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
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Re: First Time Attempt [Re: amidogen]
#23726506 - 10/10/16 08:23 PM (7 years, 3 months ago) |
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Quote:
amidogen said: Like $70 in amazon. Absolutely worth it, it's a gateway to everything you could possibly want to do in this hobby.
yeah i figured that out so far lol only thing i can do is PF tek without one, its on the list of things to get as soon as my CC gets here i had to order a new one because there was unfamiliar charges on it
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Shrooms4fun
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well that other vendor didnt do me so well that whole project got junked time to try again tho i got new syringes from a sponsor/vendor and i can actually see the specs in these ones so perhaps i can get colonization out of more than 3 jars of 16 that only started growing at one injection point.....i hae a better feeling about this.
Also got myself a PC going to try my hand at a single jar of rye berries lets see how that works also might as well practice preparation and procedure and if i botch up i only wasted 1 jar just as well as if i dont mess up it can be a master jar for others in a little while
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Shrooms4fun
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got the last of my supplies today my rye berries my ager my PC and petri dishes. YAY
so far i have rinsed the rye berries 3 times regardless because even after the first rinse the water looked very clear still so i said screw it and rinsed the crap out of them any way. this was the rye berries i had bought as reference https://www.amazon.com/gp/product/B0049YM8XU/ref=oh_aui_detailpage_o01_s00?ie=UTF8&psc=1
now they are doing their 12-24 hour soak im only planning on doing maybe 4 or 5 jars maximum to try to get the procedure down and try to reduce any possible waste, how ever if i do not have any contaminates i will from here prepare for grain to grain transfers with these few jars so i can actually expand and spawn to bulk.
i dont know if i failed at the PF tek or if it was faulty syringes i will state this tho about 4 jars of 17 between 2 and a half syringes was all that began to grow myc all 4 of those jars only had growth from 1 of the 4 injection points and beyond that it took about 2.5 or 3 weeks for even the first jar to show any signs of myc growth, the vendor also seems to have a bad reputation as well as the fact is not a site sponsor.
with all these things in mind as well as the fact that i have plenty of things left to keep running the PF tek for a while should i start experimenting with grains while working on mastering the PF tek or should i just jump right into grains and leave the PF tek behind?
i could see a few reasons why i may keep using PF tek but mostly because i still have supplies for it and it is simple enough to get basic cultivation skills down as well.
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