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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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i know its going to sounds dumb but its really by look/feel for me.
i never used gypsum with pf cakes, only grains. just realized i have no clue if the amount needed effects the pf cake texture one way or the other but 1/4 cup for 12 pints sounds reasonable.
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Shrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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Re: First Time Attempt [Re: mushboy]
#23673857 - 09/24/16 12:34 PM (7 years, 4 months ago) |
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all right well i got all the things out and around on the table lets see how this gos i have yet to try this even tho i have 36 1/2 pint jars ready to go rather than waste materials im just going to try in 12 jar increments until i get it down
EDIT: i skipped the gypsum for now and using my calculations i was able to load 17 1/2 pint jars. The reason i have skipped the gypsum for now was because i was concerned about it taking away from the moisture content as it is a dry powder so i figured i would stick to the tek's instructions at this point in time. Perhaps soon i will just do a simple 5 jar grow and try the gypsum and see what happens.
UPDATE: the jars have been steamed and are now cooling might be able to knock them up tonight as i removed them from the pot to cool mostly because i had to run the jars in 2 sets because i could not fit them all in the pot the first time i sterilized
UPDATE 2: Everything is knocked up they cooled from about 630pm until about 10pm and I Knocked them at about 11:30pm lets see how this gos i will keep this thread updated
Edited by Shrooms4fun (09/24/16 10:39 PM)
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Shrooms4fun
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Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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hmmmm how long should this take if going from spores? it has been about 6 days and im not getting any signs of anything yet.
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markisses


Registered: 09/17/06
Posts: 68
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Quote:
korn16ftl3 said: hmmmm how long should this take if going from spores? it has been about 6 days and im not getting any signs of anything yet.
Mine took 10 days with 8 month old MS on finely ground brown rice and vermiculite. I believe it could take up to 3 weeks. If it has been longer than 3 weeks, start a fresh batch.
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Shrooms4fun
Stranger

Registered: 11/27/15
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Last seen: 6 years, 11 months
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Re: First Time Attempt [Re: markisses]
#23705889 - 10/04/16 07:13 AM (7 years, 3 months ago) |
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Quote:
markisses said:
Quote:
korn16ftl3 said: hmmmm how long should this take if going from spores? it has been about 6 days and im not getting any signs of anything yet.
Mine took 10 days with 8 month old MS on finely ground brown rice and vermiculite. I believe it could take up to 3 weeks. If it has been longer than 3 weeks, start a fresh batch.

Well I'm on a week and a half now with no signs of anything so I guess 3 weeks means so far I'm still in the green.....I might buy a premade LC jar from one of the sites vendors and get that started and from there inject the pf jars from the LC myc in order to speed things along a bit, also of this fails I won't waste syringe after syringe.
A note that should probably be made I used normal unsoftened tap water (also note I have well water) in the recipe, would/could this be an issue or cause issues with this project?
Edited by Shrooms4fun (10/04/16 07:17 AM)
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markisses


Registered: 09/17/06
Posts: 68
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I wouldn't worry about speeding up the one's you have knocked up by trying to make some LC to reinoculate. If you do decide to make some LC, just use it on a fresh batch. Remember that using a LC can still allow contaminates to sneak into your substrate. Maybe try your hand at agar to guarantee you're inoculating them with some clean culture.
Checkout Violet's Microwave No-Pour Agar or Pastywhyte's Easy Agar Tek.
I don't believe your well-water would cause any issues. When I had a well back in the day, the water was similar to bottled spring water but it had more minerals in it, which would not affect the mycelium in a bad way. I use bottled spring water on the ones in that pic.
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Shrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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Re: First Time Attempt [Re: markisses]
#23709003 - 10/05/16 06:21 AM (7 years, 3 months ago) |
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Quote:
markisses said: I wouldn't worry about speeding up the one's you have knocked up by trying to make some LC to reinoculate. If you do decide to make some LC, just use it on a fresh batch. Remember that using a LC can still allow contaminates to sneak into your substrate. Maybe try your hand at agar to guarantee you're inoculating them with some clean culture.
Checkout Violet's Microwave No-Pour Agar or Pastywhyte's Easy Agar Tek.
I don't believe your well-water would cause any issues. When I had a well back in the day, the water was similar to bottled spring water but it had more minerals in it, which would not affect the mycelium in a bad way. I use bottled spring water on the ones in that pic.
I wasent going to reinoculate the older jars if those dont pan out after 2 months or how ever long I decide to let them set (well past the time they should anyhow) then I'll dump and clean them. I was just going to make an LC to colonize new brf jars quicker. I have looked into ager as well I've seen a few places that sell dishes prepoured in 4 sets or so and have considered goino that route just as an easy way to just check it out right quick. On a plus note I would assume I did sterilization correctly because absolutely nothing has grown as of yet......? Would that be as safe assumption in this case?
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Shrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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Well I checked the other 12 jars that I havery been leaving alone (I have a set of 4 of the 16 that I habe been routinely checking)I found 1 jar finally starting to colonize and another contaminated with green mold all others appear to be currently not effected as of now with all jars being inoculated on 9/24 before midnight. So I guess there is still hope for the remaining 14 jars
Look at the white spot a little ways down from the lid it's not real easy to see just yet but this is the good one:

Contaminated jar:
Edited by Shrooms4fun (10/05/16 10:46 PM)
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Shrooms4fun
Stranger

Registered: 11/27/15
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Last seen: 6 years, 11 months
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another one started colonizing today im starting to get excited now :-)
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Shrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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Here is food for thought, So far I only have 2 jars of 18 colonizing (one contamination and 1 I screwed up right from the get go) if these for some reason turn out to be the only 2 cakes that turn out for me would it be possible to transfer from cake to ager and if so where would I do that step in the process? Before or after birthing? This also might seem like a silly question but just for self reassurance after taking let's say 5 ager samples from the cake I can still fruit the cakes after taking the sample correct?
Edited by Shrooms4fun (10/09/16 08:36 AM)
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Shrooms4fun
Stranger

Registered: 11/27/15
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Last seen: 6 years, 11 months
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*bunp
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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Re: First Time Attempt [Re: amidogen]
#23724752 - 10/10/16 10:36 AM (7 years, 3 months ago) |
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Ya I'm aware of the cloning option as well how ever I did not think about that when I wrote that post thanks for reminding me....I think the only thing that has me concerned about cloning is how to select a good fruit/parent
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
Stranger

Registered: 11/27/15
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Last seen: 6 years, 11 months
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Re: First Time Attempt [Re: amidogen]
#23725167 - 10/10/16 12:50 PM (7 years, 3 months ago) |
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That simple to select them eh? Ya I have plenty of spores left....I'm just not sure if I like the syringes....I got them from a vendor and apparently they are shitty vendors from what I have read....so I'm not really sure how this grow is going to turn out. I have 2 LC jars premade from out grow on the way as well so maybe inject like the whole 6 cc of the partial syringe in that? Is there such a thing as over doing an MS injection for an LC?
Edited by Shrooms4fun (10/10/16 06:59 PM)
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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Re: First Time Attempt [Re: amidogen]
#23726212 - 10/10/16 07:00 PM (7 years, 3 months ago) |
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Quote:
amidogen said: Why do you keep spending $ ordering premade shit? If this site should have taught you anything it's that it's cheaper and more effective to make your own of anything to do with this hobby.
simpler for the time being and i still have yet to order a PC which i really do need to do
Quote:
amidogen said: If you're trying to make liquid anything, you need to use a clean culture to do it or you will waste your time, spores, and sub. The only way to get a clean culture is agar.
By the way, even though we all hate the vendor you got your shit from, you still need to keep vendor names out of cult posts.
Can i just drop a piece of clean culture strait from the ager after a few transfers right into an LC jar with sterile scalpel and an open lid LC jar inside a SAB? From my understanding im not supposed to EVER open the lid of a sterile jar of any sort or as little as possible
Edited by Shrooms4fun (10/10/16 07:12 PM)
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:10 AM)
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Shrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
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Re: First Time Attempt [Re: amidogen]
#23726506 - 10/10/16 08:23 PM (7 years, 3 months ago) |
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Quote:
amidogen said: Like $70 in amazon. Absolutely worth it, it's a gateway to everything you could possibly want to do in this hobby.
yeah i figured that out so far lol only thing i can do is PF tek without one, its on the list of things to get as soon as my CC gets here i had to order a new one because there was unfamiliar charges on it
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Shrooms4fun
Stranger

Registered: 11/27/15
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Last seen: 6 years, 11 months
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well that other vendor didnt do me so well that whole project got junked time to try again tho i got new syringes from a sponsor/vendor and i can actually see the specs in these ones so perhaps i can get colonization out of more than 3 jars of 16 that only started growing at one injection point.....i hae a better feeling about this.
Also got myself a PC going to try my hand at a single jar of rye berries lets see how that works also might as well practice preparation and procedure and if i botch up i only wasted 1 jar just as well as if i dont mess up it can be a master jar for others in a little while
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