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OfflineShrooms4fun
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Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
Re: First Time Attempt [Re: Boogieman47]
    #23584990 - 08/27/16 08:29 PM (7 years, 5 months ago)

As a quick update I have my half pint jars all made up but that making an sfd guide was I'm sure accurate and is completely doable however I need to develop a nack for it so I just ordered some SFD's from one of the vendors at the top of this site. After a little bit of work with the thin metal of the jar lids the larger GA hole proved to be a serious challenge as the drill bit kept shredding the hole as it passed threw the metal.....so in hind sight I think it would be a good idea to invest in a punch that is capable of punching holes in thin metals.



Edited by Shrooms4fun (08/27/16 08:52 PM)


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InvisibleTuhdoww
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Registered: 08/23/16
Posts: 300
Re: First Time Attempt [Re: bodhisatta]
    #23585105 - 08/27/16 09:08 PM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Two quarts MS AA+ on oats
Two quarts MS ESS on oats
54quart sterlite locking lid tub
One brick coir + quart vermiculite
No gypsum

First flush dry 5.5 oz


If spores don't work don't blame the spores. There's enough genetics in even a limited MS grow (spores diluted before inoculation or a couple agar transfers IE less strains) that you can push it to perform if given adequate conditions (clean spawn, proper moisture and moisture maintenance of needed, and fruiting conditions)




That made for a nice pic. That's cool dude!


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OfflineShrooms4fun
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Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
Re: First Time Attempt [Re: Tuhdoww]
    #23657262 - 09/18/16 10:44 PM (7 years, 4 months ago)

So I now have 36 half pin jars made and ready for substrate and inoculation.

A few quick questions before I get as far as steam sterilization,

1) will it hurt anything if I sterilize beyond an hour? For example  1.5 hours?

2) I read a lot of people using oust in both the SAB and in the air around them to kill floating contams in the air, would lysol be a suitable substitute?

3) what is the recommended additive of gypsum to the 5 cake recipe given in the video I linked in the OP?

4) I haven't read or recall hearing in the video a recommended cool down period after steam sterilization. Would it be safe to sterilize all the jars and noc them the next day?

5) when inserting the needle into the injection point I'm assuming the needle is to pass threw the top layer of verm into the actual brf substrate. Am I correct in this assumption as none of the demonstratin videos really show this detail nor specify


Edited by Shrooms4fun (09/18/16 10:51 PM)


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InvisiblemushboyMDiscord
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Re: First Time Attempt [Re: Shrooms4fun]
    #23657324 - 09/18/16 11:11 PM (7 years, 4 months ago)

Quote:

Kjay123 said:
My total dry weight on my first grow right now is at 2.5 oz, all still going , my monotub is just starting to pin again ,
Cakes didn't put out too much , but at least they are producing , down to 11 out of 12 jars still going ,  ALL MS SYRINGES, and my grain bags worked great with multi spore ,    my first topic , I had multiple "pros" treat me like a dick and tried to convince me all my shit was contaminated,  real welcoming place too guys , I wonder how many newbies you guys put off to this hobby  ,  not directed towards everyone , just some of you jerk offs




got pics?


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OfflineShrooms4fun
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Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
Re: First Time Attempt [Re: mushboy]
    #23657852 - 09/19/16 05:50 AM (7 years, 4 months ago)

Wow so much animosity around here or at least on this thread


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Offlinewtfcrazymofo
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Registered: 07/26/15
Posts: 1,201
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Re: First Time Attempt [Re: Shrooms4fun]
    #23657914 - 09/19/16 06:15 AM (7 years, 4 months ago)

1.no
2.no just make sure the sab walls are wet with a soapy water solution and wait for the dust to fall that is in the air for 15 or so in a no draft room.
3.
what link? there is no said amount of gypsum just a random ass handful, or like 4-5%
4.yes
5.yes, and do the videos use ships?

seemes to me like you got grain jar lids and not pf tek lids.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineShrooms4fun
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Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
Re: First Time Attempt [Re: wtfcrazymofo]
    #23657943 - 09/19/16 06:42 AM (7 years, 4 months ago)

Quote:

wtfcrazymofo said:
1.no
2.no just make sure the sab walls are wet with a soapy water solution and wait for the dust to fall that is in the air for 15 or so in a no draft room.
3.
what link? there is no said amount of gypsum just a random ass handful, or like 4-5%
4.yes
5.yes, and do the videos use ships?

seemes to me like you got grain jar lids and not pf tek lids.




3) this video is the 5 cake formula I will be following


5) ships? Don't believe I have herd this term before.

Lids: from my understanding all lids need a port for injection and GE. Will/does the performance of colonization vary from tek yo tek based on lids aline as long as these 2 conditions are met? All guides for lids were pretty simple and basically the same as these aside from the lids in the above linked video which uses 4 holes used as injection ports then later covered with microspore tape (medical tape). Any ideas on how/if the lids I have made will function vs the 4 hole lids used in the video?


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Offline7munkee
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Re: First Time Attempt [Re: Shrooms4fun]
    #23660268 - 09/19/16 11:04 PM (7 years, 4 months ago)

Self Healing Injection Ports, or ships.  I use high temp silicone and it works well.


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Offlinewtfcrazymofo
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Registered: 07/26/15
Posts: 1,201
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Re: First Time Attempt [Re: Shrooms4fun]
    #23660630 - 09/20/16 03:38 AM (7 years, 4 months ago)

As long as you can have more than one inoculation point (4) it should work.  The top layer of dry verm is for a contam barrier so with your whatman lids you wont really need it, but you should do it anyway.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: First Time Attempt [Re: Shrooms4fun]
    #23660639 - 09/20/16 03:44 AM (7 years, 4 months ago)

Quote:

korn16ftl3 said:
As a quick update I have my half pint jars all made up but that making an sfd guide was I'm sure accurate and is completely doable however I need to develop a nack for it so I just ordered some SFD's from one of the vendors at the top of this site. After a little bit of work with the thin metal of the jar lids the larger GA hole proved to be a serious challenge as the drill bit kept shredding the hole as it passed threw the metal.....so in hind sight I think it would be a good idea to invest in a punch that is capable of punching holes in thin metals.





Yeah I think thsoe lids suck for this tek. you wont be able to get the spores all around the instde of the jar. Just use the lids in the video.  It has been done 1000s of times with metal lids upsidedown with 4 holes.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineShrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
Re: First Time Attempt [Re: Shrooms4fun]
    #23660855 - 09/20/16 07:09 AM (7 years, 4 months ago)

Quote:

korn16ftl3 said:
Quote:

wtfcrazymofo said:
1.no
2.no just make sure the sab walls are wet with a soapy water solution and wait for the dust to fall that is in the air for 15 or so in a no draft room.
3.
what link? there is no said amount of gypsum just a random ass handful, or like 4-5%
4.yes
5.yes, and do the videos use ships?

seemes to me like you got grain jar lids and not pf tek lids.




3) this video is the 5 cake formula I will be following


5) ships? Don't believe I have herd this term before.

Lids: from my understanding all lids need a port for injection and GE. Will/does the performance of colonization vary from tek yo tek based on lids aline as long as these 2 conditions are met? All guides for lids were pretty simple and basically the same as these aside from the lids in the above linked video which uses 4 holes used as injection ports then later covered with microspore tape (medical tape). Any ideas on how/if the lids I have made will function vs the 4 hole lids used in the video?




Screw it I just made standard pf tek lids with the 4 homes anyone e else have any other input from up above there?


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OfflineShrooms4fun
Stranger

Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
Re: First Time Attempt [Re: Shrooms4fun]
    #23663117 - 09/20/16 08:46 PM (7 years, 4 months ago)

Here is a thought how about I use an under the bed sterilite  tote like the one in the picture I'm going to upload to store all the pf jars in? I mean would this restrict airflow too much? Should I drill some half inch holes threw the sides or something? This is just a thought for easy storage and mobility of 36 pf jars feedback would be appreciated :-) I have pets and hard floors so dirt getting kicked around is pretty common



Edited by Shrooms4fun (09/20/16 09:03 PM)


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Invisibledankington
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Folding@home Statistics
Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: First Time Attempt [Re: Shrooms4fun]
    #23663256 - 09/20/16 09:22 PM (7 years, 4 months ago)

Looks good dude. And those lids would have been fine for PF tek, as long as you make sure there's GE going on. I'd have pushed in the needle against the glass so I could see the inoculation point, then rolled the jar around a bit to spread the solution. Just a couple drops.

:goodluck:


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OfflineShrooms4fun
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Registered: 11/27/15
Posts: 63
Last seen: 6 years, 11 months
Re: First Time Attempt [Re: dankington]
    #23664261 - 09/21/16 07:14 AM (7 years, 4 months ago)

Quote:

dankington said:
Looks good dude. And those lids would have been fine for PF tek, as long as you make sure there's GE going on. I'd have pushed in the needle against the glass so I could see the inoculation point, then rolled the jar around a bit to spread the solution. Just a couple drops.

:goodluck:




It was just a few bucks to buy more lids I'm not to concerned about it I'll just use the others if I decide to go the grain route seems if the pf tek is simple the  perhaps I should just mass produce the half pint jars and spawn to bulk coir with the 50/50 I've been reading about. At least if I get the pf tek down contam free and the myc is resilient after 100% colonized as well as coir and verm being contam resistant I should be able to do a whole tub with 35 or 36 jars easily from start to finish pretty much contam free I would imagine.

As far as the monotub idea gos I read a lot about people dunking between flushes....I'm assuming that means harvest then remoisten the sub? If that is the case how does one get it back toward the field capacity range?


Edited by Shrooms4fun (09/21/16 07:23 AM)


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Invisibledankington
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Re: First Time Attempt [Re: Shrooms4fun]
    #23664423 - 09/21/16 08:57 AM (7 years, 4 months ago)

I hightly recommend fruiting cakes as cakes. You won't notice any appreciable yield increase by spawning the cakes, and cakes are always riddled with contams.

Image courtesy of Adden


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OfflineShrooms4fun
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Registered: 11/27/15
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Re: First Time Attempt [Re: dankington]
    #23664689 - 09/21/16 10:55 AM (7 years, 4 months ago)

Quote:

dankington said: cakes are always riddled with contams.





I'm curious about this, isent that why we sterilize?


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Invisibledankington
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Re: First Time Attempt [Re: Shrooms4fun]
    #23665047 - 09/21/16 01:21 PM (7 years, 4 months ago)

It's the nature of the method. Mushrooms don't grow in sterile environments, so their spores aren't either. Spore syringes are as clean as they can get them.

Essentially, BRF cakes are so nutritious, that the myc is often able to perform, despite all the bacteria present. That's why cakes aren't very good for spawn. You want your spawn to be as clean as possible. Minimize all the vectors you can.


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OfflineShrooms4fun
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Registered: 11/27/15
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Last seen: 6 years, 11 months
Re: First Time Attempt [Re: wtfcrazymofo]
    #23673753 - 09/24/16 11:53 AM (7 years, 4 months ago)

Quote:

wtfcrazymofo said:
there is no said amount of gypsum just a random ass handful, or like 4-5%





So as per this site:
https://www.shroomery.org/5143/What-recipe-should-can-I-use-for-PF-cakes

Quote:

The basic PF recipe is 1/4 cup brown rice flour (measure the flour itself, not the brown rice if you grind it yourself), 1/2 cup vermiculite(fine is preferred), and 60ml (4 tablespoons) of water per jar.




and according to the recommendations of about 6% gypsum and if my math is correct (someone please correct me if it is because im setting up to do this a soon as i have confirmation).

1/4 cup + 1/2 cup + 4 tablespoons = 1 cup total

6% of 1 cup = 14.1952942 ml

14.1952942 ml = 2.880000933267 tsp

so for 1 cake and the recipe above i would go: 1/4 cup brown rice flour, 1/2 cups vermiculite, 4 tablespoons of water, and 3 tsp of gypsum, per jar.

doing 12 jars this comes out to be:

3 US cups brown rice flour, 6 US cups vermiculite, 48 US tablespoons of water (reduced is 3 cups), 36 tsp gypsum (reduces to 3/4 cups)

this in total should yield me 12 jars of PF cakes. I do not know if i should have included the water in the total volume of the recipe to calculate the % of gypsum as i dont think the water actually adds volume just density.

so does all this seem correct?


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InvisiblemushboyMDiscord
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Re: First Time Attempt [Re: Shrooms4fun]
    #23673763 - 09/24/16 11:56 AM (7 years, 4 months ago)

for a batch of 7 quarts i just use too spoonfuls of gypsum..it
doesnt have to be exact. your not making a rue.


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OfflineShrooms4fun
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Re: First Time Attempt [Re: mushboy]
    #23673782 - 09/24/16 12:00 PM (7 years, 4 months ago)

Quote:

mushboy said:
for a batch of 7 quarts i just use too spoonfuls of gypsum..it
doesnt have to be exact. your not making a rue.




so even a 1/4 cup for 12 pint jars would work?

should i spray the jars out with rubbing alcohol before i use them they are new or will sterilizing take care of all of that for me?


Edited by Shrooms4fun (09/24/16 12:02 PM)


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