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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Galandoi Truffle Growers Please Help :)...
#23556285 - 08/19/16 09:20 AM (7 years, 5 months ago) |
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Hi Guys,
I am just looking for some expert advice relating to what to do with these jars of galandoi. On the 25/7, just about a month in I have a jar that had an agar wedge placed in and it looks like it is colonising nicely...

Now if you zoom in and take a close up of the grain, it looks moist on the outside and nicely puffed out:
So only a few days later, 29/7 I drop a wedge into another jar, but it is colonising slowly, I give it a bit of a shake and not much is happening:
Now if you zoom in on that one the grain looks kinda dry, like the mycelium is not getting enough water or doing that much:

So regarding advice - (and I appreciate any guidance), do you think I should just be patient with the second jar and let it colonise or dump out the grains and re-boil it to ensure that the grains are better hydrated and then drop another wedge in?
Thanks for your help...
-------------------- Why reinvent the wheel when someone here has already done the science?
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Galandoi Truffle Growers Please Help :)... [Re: AgarStudent]
#23556328 - 08/19/16 09:47 AM (7 years, 5 months ago) |
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https://www.shroomery.org/forums/showflat.php/Number/15373619#15373619
Stones love a drier grain, so if it's moving slow on drier grain I suspect you should start over.
Try more trials like start a dozen jars from a dozen agar plates.
Check my journal called growing stones for a load of quotes and links to posts from many large scale stone growers.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Re: Galandoi Truffle Growers Please Help :)... [Re: filthyknees]
#23556365 - 08/19/16 10:03 AM (7 years, 5 months ago) |
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Thanks, I had a look at your journal and it sounds neat. So you are saying that stones can actually form on the agar plates? I have only ever seen white fluffy mycelium and transferred wedges of that. That should turn into stones eventually? It is a cool thread so I will have to read it again.
-------------------- Why reinvent the wheel when someone here has already done the science?
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Galandoi Truffle Growers Please Help :)... [Re: AgarStudent]
#23556425 - 08/19/16 10:29 AM (7 years, 5 months ago) |
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It's very common to have stones form on agar during colonization or shortly after.
Sclerotia is often off white. I have some stone plate pics in my gallery folder called second log.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
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Re: Galandoi Truffle Growers Please Help :)... [Re: filthyknees]
#23558227 - 08/19/16 08:33 PM (7 years, 5 months ago) |
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Thanks for the suggestion,
Now I did check out the 'Lets Grow Mushrooms' video on strain isolation and have some results relating to my first transfer (Tx1) and second transfer (Tx2) from two stone producers including Galandoi and Chico Nindo (which I think is meant to be a sub-strain of mexicana?)
I can't yet see any areas that are producing stones, but is it possible to make a suggestion which areas of these agar plates might be most likely to produce stones?
First I have galandoi:
And then I have chico nindo:

Any feedback appreciated
-------------------- Why reinvent the wheel when someone here has already done the science?
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