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Bernard
Cultvr - Not Necessarily Trusted



Registered: 05/08/16
Posts: 226
Last seen: 2 years, 7 months
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Starting Agar - Testing Potency
#23556013 - 08/19/16 06:51 AM (7 years, 5 months ago) |
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I've done a couple of BRF cakes and I'm tired of this multi-spore bull crap. My mushrooms make great flushes, look good and healthy, but they don't contain much magic.
So, I've gathered materials for agar (practiced making a batch) and I've got another crop of MS cakes currently fruiting. My intention is to clone some of the fruits from these cakes. My goal is to get a mushroom that has better potency.
I've read quite a bit about testing potency, and here's what I've gathered...accurate testing of "actives" requires some expensive and serious equipment. Most people test potency by ingesting and then grading their trip.
Let me tell you, this is going to take me years. I only get the opportunity to trip every 6 - 8 weeks (I know, I know, it's just the way life is for me). I DO NOT sell or otherwise distribute my shrooms to anyone. They are strictly for myself, so feedback from others is not possible.
Question 1: Is the above assessment of testing true? There's no easy, better way to test for potency?
Question 2: Can you get an idea of potency by judging the bluing characteristics? I noticed once when I was cutting a cap for a spore print that the alcohol on my scissors seemed to bring out the bluing. Is it possible to maybe swab a shroom with alcohol and see how much it blues?
Question 3: Should I start a MS on agar first, then create 10 or so isolates from that - bring them to fruit and then make a decision from that, or is it easier to just clone the fruit from a MS BRF cake?
I've spent quite a bit of time reading on this board, but I don't know that anyone's used an "alcohol bluing" test to judge potency.
Anybody?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: Starting Agar - Testing Potency [Re: Bernard]
#23556022 - 08/19/16 07:01 AM (7 years, 5 months ago) |
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1. Pretty much. The mouth is the instrument we use to test potency.  2. No, bluing has no relation to potency at all, not even a little. If it were even a little, we'd skip #1. 3. You should really just take clones, then eat the fruits you cloned, and judge that way, now you immediately have a workable culture instead of isolating shit that might not even fruit. Making isolates is a lot of work, and I don't really consider it worthwhile if all you're doing is trying to get a fairly consistent culture.
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Bernard
Cultvr - Not Necessarily Trusted



Registered: 05/08/16
Posts: 226
Last seen: 2 years, 7 months
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Re: Starting Agar - Testing Potency [Re: Inocuole]
#23558361 - 08/19/16 09:18 PM (7 years, 5 months ago) |
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OK, next question...How much difference does it make to have only one strain in a cake or tub? I've read where ms cakes can sort of fight themselves because there's more than one strain trying to grow off of the same food source.
Will moving to a clone help potency and/or yield even if the clone was average?
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Bernard
Cultvr - Not Necessarily Trusted



Registered: 05/08/16
Posts: 226
Last seen: 2 years, 7 months
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Re: Starting Agar - Testing Potency [Re: Bernard]
#23558372 - 08/19/16 09:22 PM (7 years, 5 months ago) |
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Also, just to get my terminology right,
Clone is a copy of a fruit
Is that not an isolate, or does an isolate have to come from a culture?
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mrmazdarx9
Pffffttt


Registered: 05/15/16
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Re: Starting Agar - Testing Potency [Re: Bernard]
#23558377 - 08/19/16 09:23 PM (7 years, 5 months ago) |
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Clone is not an isolate
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Boogieman47
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Registered: 03/05/16
Posts: 9,712
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Re: Starting Agar - Testing Potency [Re: mrmazdarx9]
#23558659 - 08/19/16 10:53 PM (7 years, 5 months ago) |
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You can isolate from MS or a clone but like noc says it's a pain you may have 100 isolates and only one is worth while and you just wasted 100's of hours and a shit load of grain and sub for what you could just keep fruiting off the multi spore cultures whether it's from spores or clones
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Starting Agar - Testing Potency [Re: Boogieman47]
#23558998 - 08/20/16 01:43 AM (7 years, 5 months ago) |
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An isolate is a single strain, just one set of genetics. A clone can still have many sets of genes within it. It's easier to isolate from a clone than from MS.
Should definitely not put one cake per fruiting chamber, but definitely one variety per substrate if you want reliable results. If you want to try to mix them, go ahead, just expect some form of genetic variation down the line.
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Bernard
Cultvr - Not Necessarily Trusted



Registered: 05/08/16
Posts: 226
Last seen: 2 years, 7 months
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Re: Starting Agar - Testing Potency [Re: Inocuole]
#23559435 - 08/20/16 09:18 AM (7 years, 5 months ago) |
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Now I'm really confused. If I use a spore syringe to start a brf cake, there will most likely be many different strains fruiting on that cake. If I pick the best mushroom, throw it on agar, the resulting mycelium should produce very close copies of the original chosen mushroom. No? Is that not the fastest, easiest way?
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MysticMoteToter



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Re: Starting Agar - Testing Potency [Re: Bernard]
#23559566 - 08/20/16 10:09 AM (7 years, 5 months ago) |
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Imo an easy yet effective way to test potency is i eat just 1g dried and if it gives a nice stone, i know it'll be good at a full dose. although in really good fruits 1g is actually mildly psychedelic. Eating just a 1g tester fruit allows you to get things done easier, (i wouldnt go to work, but household chores, errands,etc are no problem), because your not fully tripping its easy to do every week.
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Pastywhyte
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Registered: 09/15/12
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Re: Starting Agar - Testing Potency [Re: Bernard]
#23559577 - 08/20/16 10:14 AM (7 years, 5 months ago) |
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I shouldn't say this but do bulk and grow APE. Some mini monos would be killer. Find a good APE clone and you will have all the potency you need forever.
Okay now let's get this straight, cubes are cubes. But APE is a great place to start.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Starting Agar - Testing Potency [Re: Pastywhyte]
#23559591 - 08/20/16 10:21 AM (7 years, 5 months ago) |
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With non PE cubes aim for 1dry gram makes you trip pretty good.
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Bernard
Cultvr - Not Necessarily Trusted



Registered: 05/08/16
Posts: 226
Last seen: 2 years, 7 months
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Re: Starting Agar - Testing Potency [Re: bodhisatta]
#23562615 - 08/21/16 10:41 AM (7 years, 5 months ago) |
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How about one of my earlier question...how much do you gain from a potency and yield standpoint by going to an isolate? If you had a strain that was only average, would you see improvements simply because there was no competition for nutrients?
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bodhisatta 
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Registered: 04/30/13
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Re: Starting Agar - Testing Potency [Re: Bernard]
#23562624 - 08/21/16 10:43 AM (7 years, 5 months ago) |
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No, you have to test your isolates. Rarely do you find a potent isolate. Most isolates are garbage
I strongly suggest clones until you're good enough with agar to worry about isolates in your spare time. I don't fuck with isolates at all not worth my time at all
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