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Expandingmind900
Newbie



Registered: 10/29/15
Posts: 10
Loc: Sweden
Last seen: 7 years, 4 months
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LC question
#23553590 - 08/18/16 02:56 PM (7 years, 5 months ago) |
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I have a pint of LC made 18 days ago. Im wondering if I should shake the jar vigorously before getting it into a syringe(so the myc breaks down into tiny pieces in the water) or do I try to keep the myc intact and thick when I get it into my syringe?
Also the dark stuff you see in the pic, on the edge of the surface. Is that a contam? Looks like white myc grows on it. Hmmm
Looks lite this

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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Toss that shit. How did you start this, spores?
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Expandingmind900
Newbie



Registered: 10/29/15
Posts: 10
Loc: Sweden
Last seen: 7 years, 4 months
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Yes with spores
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
Expandingmind900 said: Yes with spores
Big waist of spores And a terrible idea And a waist of time And an extra step
https://www.shroomery.org/forums/showflat.php/Number/23540508#23540508
Quote:
Trusted Cultivator said: exactly.
https://www.shroomery.org/forums/showflat.php/Number/21365919#21365919
take a look at that post
a spore print is used to make a spore syringe.
there might be 10 trillion spores in your print and say 100 bacterium. if you use your spore syringe on a cake chances are you'll have less than 1 bacterium in your cake, which means success and even if say you get one or two bacterium in your cake, you'll still get success
however if you put your spore syringe into a LC and EVEN ONE bacterium gets into the LC it will fuck the whole LC. being a nutritional liquid the bacteria just multiplies and spreads through the whole thing, you just wasted your spores.
going back to the thread I linked too, like pasty said there's too many spores in a syringe. so rather than make a LC just take your spore syringe and dilute it down 1:10 or 1:100. you can take one spore syringe and turn it into 10 for instance. those 10 new syringes will work just fine since they still have way more spores than you need per drop. not only that but you diluted down any contaminants that may be present which is far more useful than trying to get a clean LC from spores.
your time table would be like this
spores to cakes, then cakes to SGFC rather than spores to LC then LC to cakes then cakes to SGFC you add a step and time your cakes form the SGFC will make more spores anyway so you don't need to extend your spores, and if you do especially not with LC
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Sorry to see that went bad. I suggest taking spores to agar and cleaning it up, then you can take it to LC, or better yet if you are not in a rush to take wedges of the agar to grain jars and expand from there.
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Expandingmind900
Newbie



Registered: 10/29/15
Posts: 10
Loc: Sweden
Last seen: 7 years, 4 months
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Well ok. Thx for the info. I'm still gonna try on a jar or two and see how it goes. And i only injected like 1-2 cc of spores so not that big of a waste:P
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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If you use that on a jar its going to get so gross you'll probably toss the jar glass and all
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Good luck 
Edit: decided to share some knowledge
If you're going to insist on using a spore syringe to make LC, 1-2cc of solution is too much. If you dont care about wasting your time or spores maybe you should make a new batch of LC rather than wasting perfectly good substrate as well and hope for the best? But that LC is clearly fubed..
Agar will save you a headache in the long run but you already know that.
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
Edited by ComebackKid (08/18/16 04:04 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Seriously you're going to do it anyway? It has dark shit in it. Mycelium is not dark shit. That is bacteria and you're like "fuck it, I'm gonna test it anyway"? Some people...
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h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
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Quote:
Expandingmind900 said: Well ok. Thx for the info. I'm still gonna try on a jar or two and see how it goes. And i only injected like 1-2 cc of spores so not that big of a waste:P
Don't waste any more, this is what happens
https://www.shroomery.org/forums/showflat.php/Number/23522774#23522774
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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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I think those are mold colonies growing on the top of that liquid. No way dude, LC is iffy to use anyways because you can't see the contamination hence testing it on agar before using it, that is clearly awful, but it would be a learning experience if you really want to dump that into some sterilized grain, shit dude why bother sterilizing the grain?
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