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OfflineKenetic
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Quick Agar question
    #23553399 - 08/18/16 02:00 PM (7 years, 5 months ago)

So....I'm going to cook up my first batch of agar today, using the pour method into petri dishes, and I am unclear on one point:

Should I wrap the dishes after I pour and before the agar solidifies, or just leave them in the SAB and wrap them up after I inoculate them?


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InvisiblebodhisattaMDiscordReddit
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Re: Quick Agar question [Re: Kenetic]
    #23553405 - 08/18/16 02:04 PM (7 years, 5 months ago)

I would like to see you wrap one before it solidified.

You can put them back info the sleeve they came out of after they solidify. Or Ziploc, or wrap but then you just have to unwrap and re wrap and that's dumb

You could just read the agar teks


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InvisibleJacobStorm
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Re: Quick Agar question [Re: Kenetic]
    #23553408 - 08/18/16 02:05 PM (7 years, 5 months ago)

Im sorry wrap the dishes with what? For what purpose? What tek are you following mate?


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Re: Quick Agar question [Re: Kenetic]
    #23553409 - 08/18/16 02:05 PM (7 years, 5 months ago)

Do not attempt to wrap them before they solidify! You'll just slosh the agar
around and make a mess. Wait till it solidifies to wrap if you plan on
storing any for later use or wait to solidify and cool then inoc immediately and wrap
them after.


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Re: Quick Agar question [Re: JacobStorm]
    #23553420 - 08/18/16 02:09 PM (7 years, 5 months ago)

Quote:

JacobStorm said:
Im sorry wrap the dishes with what? For what purpose? What tek are you following mate?



What are you talking about?
Dishes get wrapped bro


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OfflineKenetic
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Re: Quick Agar question [Re: natedawgnow]
    #23553425 - 08/18/16 02:11 PM (7 years, 5 months ago)

Thanks for the quick replies! 

I've read that when the dishes are cooling they suck in a little bit of air and it could contaminate them.  Hence my concern.

Thanks again for clearing it up.


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Re: Quick Agar question [Re: Kenetic]
    #23553431 - 08/18/16 02:13 PM (7 years, 5 months ago)

Sucking air in on cooling is an excuse noobs like to use for failure so they do implicate their own shit methods and lack of skill.


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OfflineKenetic
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Re: Quick Agar question [Re: bodhisatta]
    #23553443 - 08/18/16 02:16 PM (7 years, 5 months ago)

well I'm about to get some practice in today!


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Re: Quick Agar question [Re: Kenetic] * 1
    #23553447 - 08/18/16 02:17 PM (7 years, 5 months ago)

I've been doing pour agar for a while and haven't had a contam related to
pouring and cooling in this fashion since i started :shrug:

I have a stack of cricket agar plates i made at least 2 months ago wrapped
in cellophane just sitting on my shelf, no ziplock or nothin, and they
still haven't contamed or or dried out.


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Re: Quick Agar question [Re: natedawgnow]
    #23553463 - 08/18/16 02:23 PM (7 years, 5 months ago)



Use one or two right away and the rest fit in with enough slack to fold the sleeve over so you can tape it down


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InvisibleJacobStorm
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Re: Quick Agar question [Re: bodhisatta]
    #23553474 - 08/18/16 02:26 PM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Quote:

JacobStorm said:
Im sorry wrap the dishes with what? For what purpose? What tek are you following mate?



What are you talking about?
Dishes get wrapped bro




Hey thats new information for me bod! Lol


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Re: Quick Agar question [Re: JacobStorm]
    #23553488 - 08/18/16 02:30 PM (7 years, 5 months ago)



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Re: Quick Agar question [Re: bodhisatta] * 1
    #23553496 - 08/18/16 02:33 PM (7 years, 5 months ago)

Well would you look at that! I like the noon wrapping thats how my Christmas presents look.


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azur said: If you like 6th grade results, then 6th grade projects are great.


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Re: Quick Agar question [Re: JacobStorm]
    #23553524 - 08/18/16 02:39 PM (7 years, 5 months ago)



Ultra noob wrap


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OfflineRooster Cogburn
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Re: Quick Agar question [Re: bodhisatta]
    #23553534 - 08/18/16 02:43 PM (7 years, 5 months ago)

How about wrapped in foil:cool:


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OfflineKenetic
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Re: Quick Agar question [Re: bodhisatta]
    #23553557 - 08/18/16 02:49 PM (7 years, 5 months ago)

That's pretty funny.  I'll be using seran wrap for now until my parafilm gets here.  One of the teks i read suggested cutting the roll into two-inch sections.  Sounds good to me!


foil?  :uhhnothanks: lol


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OfflineKenetic
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Re: Quick Agar question [Re: natedawgnow]
    #23553571 - 08/18/16 02:53 PM (7 years, 5 months ago)

Quote:

natedawgnow said:
I've been doing pour agar for a while and haven't had a contam related to
pouring and cooling in this fashion since i started :shrug:

I have a stack of cricket agar plates i made at least 2 months ago wrapped
in cellophane just sitting on my shelf, no ziplock or nothin, and they
still haven't contamed or or dried out.





Good to know!


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineKenetic
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Re: Quick Agar question [Re: Kenetic]
    #23553643 - 08/18/16 03:11 PM (7 years, 5 months ago)

Since I've already got a post up maybe I can sneak one more question in:

Is it better to cut a wedge or scrape a tiny piece of myc (with a scalpel)?

I see a lot of people cutting chunks of agar out but it seems like it might be good to scrape a smaller piece if you can..... maybe the scalpel would damage the myc by scraping?

It seems like everyone is doing the same thing for a reason, and probably a good one.  I haven't found a decent search result on this.


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Re: Quick Agar question [Re: Kenetic] * 1
    #23553745 - 08/18/16 03:49 PM (7 years, 5 months ago)

Good question I'll wait for an answer with ya


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Re: Quick Agar question [Re: ComebackKid]
    #23553777 - 08/18/16 03:55 PM (7 years, 5 months ago)

With a wedge mycelium has to crawl off so if you didn't have a clean plate or transfer you might see it quicker. You will damage mycelium scraping sure bit can't kill all of it


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