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Dosha9
Wizard of the wood


Registered: 10/22/14
Posts: 161
Last seen: 6 years, 1 month
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No fruit after 7 weeks
#23553056 - 08/18/16 11:31 AM (7 years, 5 months ago) |
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I followed franks teks and started 5 mono tubs. Two of them contaminated after about three weeks (for mistakes that I'm aware of), and the other three are still going strong, but still no fruits.
They were all started from the second transfer of a MS inoculation. I think I might have accidentally created an isolate, but the isolate is a non fruiting dud.
I've posted pictures, and all the commenters say the tubs look fine, and that I should be patient, but I need make room for my up and coming projects.
What do you guys think might have gone wrong here? Genetics?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: No fruit after 7 weeks [Re: Dosha9]
#23553100 - 08/18/16 11:45 AM (7 years, 5 months ago) |
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Takes 10+ transfers to get an isolate and a non fruiting one would be rare.
What went wrong was your spawn health, substrate moisture content, and fruiting conditions.
Every noob wants to blame it on things outside of their control. 99.9% of the time it's blamed on shit like genetics like its some way to avoid figuring out the real problem.
Then every noob says they did everything to the T correct but probably didn't
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: No fruit after 7 weeks [Re: Dosha9]
#23553105 - 08/18/16 11:46 AM (7 years, 5 months ago) |
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Check your FAE and microclimate. Since there are no pictures and no link to your other thread I can only assume it's the microclimate. Evaporation from the surface and gas exchange are two main pinning triggers
I'm curious on your transfers. What did you exactly do?
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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Dosha9
Wizard of the wood


Registered: 10/22/14
Posts: 161
Last seen: 6 years, 1 month
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Re: No fruit after 7 weeks [Re: Rosen_Rot]
#23553165 - 08/18/16 12:08 PM (7 years, 5 months ago) |
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Here's picture of my earlier thread. https://www.shroomery.org/forums/showflat.php/Number/23484663
I inoculated agar dishes with a spore print and an inoculation loop, and then transferred twice from the healthiest looking mycelium.
I also used drywall for gypsum. pretty sure it's not treated, cause the treated stuff is either pink or green. Maybe this caused a problem? Although I followed the ratios in franks teks, my substrate was really wet, so I had to keep adding vermiculite until the sub was more damp then wet. If too much moisture is the culprit, wouldn't the that have caused contams by now?
Edited by Dosha9 (08/18/16 12:14 PM)
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JesusDaMartian
★ Intergalactic Shaman ★


Registered: 04/27/14
Posts: 406
Loc: USA East Coast
Last seen: 5 months, 29 days
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Re: No fruit after 7 weeks [Re: Dosha9]
#23553217 - 08/18/16 12:33 PM (7 years, 5 months ago) |
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It could deff be the drywall . From the photos looks to wet
Edited by JesusDaMartian (08/18/16 12:35 PM)
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: No fruit after 7 weeks [Re: Dosha9]
#23553593 - 08/18/16 02:56 PM (7 years, 5 months ago) |
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I doubt it's the gypsum.
Those tubs look extremely wet and there are probably contaminants present somewhere in the bulk and could very well be why your tub is not pinning yet. Try loosening that polyfil to allow more FAE to occur. I wouldn't mist for a while either. I'd be worried about pools of water forming underneath the sub
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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Dosha9
Wizard of the wood


Registered: 10/22/14
Posts: 161
Last seen: 6 years, 1 month
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I was just looking over franks teks, and spotted a big flaw in my method: unlike frank, I fill my quart jars to brim when I measure out the water needed for my substrate. That explains why I needed to keep adding more vermiculite. oops!
He also uses only a handful of gypsum; where as I was using a half a quart of broken up drywall. I had trouble finding gypsum in my area, so that's why I went the drywall route.
Im did my pasteurization in ziplock freezer bags, but this time, I'll do it in jars like RR teaches in his DVD
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ShroomieNewbie
Stranger


Registered: 08/16/16
Posts: 55
Loc: Somewhere I shouldn't be..
Last seen: 6 months, 1 day
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Re: No fruit after 7 weeks [Re: Dosha9]
#23554029 - 08/18/16 05:00 PM (7 years, 5 months ago) |
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Quote:
Dosha9 said: I was just looking over franks teks, and spotted a big flaw in my method: unlike frank, I fill my quart jars to brim when I measure out the water needed for my substrate. That explains why I needed to keep adding more vermiculite. oops!
He also uses only a handful of gypsum; where as I was using a half a quart of broken up drywall. I had trouble finding gypsum in my area, so that's why I went the drywall route.
Im did my pasteurization in ziplock freezer bags, but this time, I'll do it in jars like RR teaches in his DVD
I'm using all of Frank's teks to a literal T. He doesn't use zip lock freezer bags for any of the teks I've seen.
Also, just take the extra step to order gypsum from a beer supplier online. Hell, they may even have it at a brew supply store around you.
I don't see a reason to cut into teks and change things. If you can't follow them the way they lay them out, why even follow them at all? You won't get the same results, and isn't that the point being a beginner?
Edited by ShroomieNewbie (08/18/16 05:02 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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get more recent pics
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: No fruit after 7 weeks [Re: cronicr]
#23554109 - 08/18/16 05:23 PM (7 years, 5 months ago) |
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I use drywall as my gypsum and it works fine.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
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Re: No fruit after 7 weeks [Re: Inocuole]
#23554430 - 08/18/16 06:50 PM (7 years, 5 months ago) |
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same here. Pull the paper off (optional), put it in a bag and bang it with a hammer. same shit, different toilet
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: No fruit after 7 weeks [Re: Kenetic]
#23554638 - 08/18/16 07:45 PM (7 years, 5 months ago) |
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I bang it with a hammer in one of my small tubs, then put it all through a strainer to catch the paper. Only powder and fine granules that will dissolve in water make it through.
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ShroomieNewbie
Stranger


Registered: 08/16/16
Posts: 55
Loc: Somewhere I shouldn't be..
Last seen: 6 months, 1 day
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Re: No fruit after 7 weeks [Re: Kenetic]
#23554640 - 08/18/16 07:45 PM (7 years, 5 months ago) |
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The cost/time on buying and smashing gypsum board is less efficient. That's basically it.
Temps change chemical makeup of some drugs too. Im no scientist, but 1500 degrees could change up some gypsum properties..
Not just that, but for beginners sake, why fix something that isn't broken? Something that is literally fool-proof. I'm not just talking about the gypsum, it's just principal.
When a guy says he followed everything to a T, when he didn't, why add "other" info. Idk. Back to basics.
Edited by ShroomieNewbie (08/18/16 07:47 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Um, because the tek doesn't say "buy gypsum" it just says "add gypsum". It's not like you have to grow your own brown rice to make BRF, there are lots of ways to acquire materials. I don't think getting the same material by a different means counts as deviating from the tek.
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ShroomieNewbie
Stranger


Registered: 08/16/16
Posts: 55
Loc: Somewhere I shouldn't be..
Last seen: 6 months, 1 day
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Re: No fruit after 7 weeks [Re: Inocuole]
#23554665 - 08/18/16 07:49 PM (7 years, 5 months ago) |
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Brown rice flour comes from brown rice. Gypsum doesn't come from gypsum board. It's the other way around. Once again.... the basics work wonders for beginners..
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: No fruit after 7 weeks [Re: cronicr]
#23554676 - 08/18/16 07:51 PM (7 years, 5 months ago) |
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Well this is why you're the newbie.
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ShroomieNewbie
Stranger


Registered: 08/16/16
Posts: 55
Loc: Somewhere I shouldn't be..
Last seen: 6 months, 1 day
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Re: No fruit after 7 weeks [Re: Inocuole]
#23554697 - 08/18/16 07:55 PM (7 years, 5 months ago) |
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You must be taking shots because you can't get your english corrected. I was giving helpful advice to a guy who didn't follow teks he says he did, resulting in going back to the basics, to get his method right.
You flamed my first thread created, now here you go again. I'm not new to life, guy.
Edited by ShroomieNewbie (08/18/16 07:56 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Newbie is literally a part of your name. I don't think I pointed anything out that wasn't already obvious. What is this about my english exactly?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
ShroomieNewbie said: You must be taking shots because you can't get your english corrected. I was giving helpful advice to a guy who didn't follow teks he says he did, resulting in going back to the basics, to get his method right.
You flamed my first thread created, now here you go again. I'm not new to life, guy.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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