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paracelsusgold

Registered: 08/15/16
Posts: 173
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glovebox: build your own easily
#23551637 - 08/17/16 10:50 PM (7 years, 5 months ago) |
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one can build their own glovebox which is necessary for inoculation purposes out of plywood and glass quite easily with a liftup top and an opening for hands as well. keep an ionizer in there while working and a small curtain sliding over the opening for the hands. tight fitting throwaway gloves are good. keep a small oil flame in there also for sterilizing tools between innoculations. set the glovebox on a sturdy table near the stove or sterilizer.
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Ya know. I see all this stuff on the news. Self parking cars. Virtual reality. Men on the moon. And I think "sheesh, why can't we just build something that lets us grow some damn cubes". Well. Eureka. Today is that day. Thank you for this fantastic tek. I'll be going out to get my plywood and plexiglass first thing.
Edited by PsilocyBen17 (08/17/16 11:39 PM)
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leech911
Man with a plan



Registered: 06/03/16
Posts: 66
Last seen: 7 years, 3 months
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Re: glovebox: build your own easily [Re: PsilocyBen17]
#23551890 - 08/18/16 12:11 AM (7 years, 5 months ago) |
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: glovebox: build your own easily [Re: leech911]
#23551913 - 08/18/16 12:18 AM (7 years, 5 months ago) |
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It should blow your mind leech that this how it was done, parecel upmost respect man, people make a glovebox out of sterilite plastic containers now so they are being snarky. It was you guys and this stuff that opened up the doors for us I think it is absolutely amazing that you guys were pumping out mushrooms with learning everything through mail corresponding and actually growing. I love it dude.
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djd101
Newbie


Registered: 07/20/14
Posts: 55
Last seen: 7 years, 4 months
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Re: glovebox: build your own easily [Re: leech911]
#23551923 - 08/18/16 12:21 AM (7 years, 5 months ago) |
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i kno how to get plywood not plexiglass beats using oven tek i suppose thanks
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kamykazi3
Stranger

Registered: 07/09/15
Posts: 89
Last seen: 7 years, 1 month
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Re: glovebox: build your own easily [Re: djd101]
#23551995 - 08/18/16 12:51 AM (7 years, 5 months ago) |
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that seems prety decent , but i have no idia how to get those materials .. is a glove box realy necessary ? what are the chances of contamination if i do inoculation on a previously desinfected bathroom ?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: glovebox: build your own easily [Re: kamykazi3] 1
#23552015 - 08/18/16 01:06 AM (7 years, 5 months ago) |
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Ron, I'd like to hear more about this, but I'd also like to share some of the more "ghetto" ways that we've been growing here for some contrast.
Most people are using still air boxes recently. They operate on the concept of still air vs a contaminant free environment. By letting all the contaminants fall to the floor and stick to the walls of the SAB, you can be sure that nothing will fall into your work when you briefly open a jar or agar plate, even if the box itself isn't totally clean.
My SAB, which works great for me, is a modified (not by much) version of this one.
http://www.shroomery.org/forums/showflat.php/Number/20048771#20048771
I get pretty good results with agar and grain by using it.
  
I'm interested in the idea of a glove box that actually works, but lately the consensus has been that the possibility of your box creating drafts which could bring contamination into your work when you move the hands/gloves around is high enough to negate the benefit you'd otherwise receive by having it completely closed off to open air. There's also a lot of talk of flames inside the box creating further updrafts which can cause contamination to ride its way into your work. I have not tested these theories because they appear sound to me and I get great results the way I do now. But I'm interested in some examples of a design that doesn't run into these issues or has a way around them. Is the entire inside of the box sterile? How can this be accomplished if so? During work with agar and grain I never do much cleaning of my box or my surroundings, since the still air negates the need for this, so this is a completely different approach.
I wouldn't recommend inoculating in a bathroom, that's the dirtiest room of the house.
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: glovebox: build your own easily [Re: Inocuole]
#23552082 - 08/18/16 01:56 AM (7 years, 5 months ago) |
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Great way of approaching that inoc. Hey Ron, i'm interested if and when laminar flow hoods were implemented when you were growing?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Well, TMC has pictures of people working in front of flowhoods and it was released in '83 so they have been around for quite a while.
I think PPP also works with a glove box. Not 100% sure if it was him but I remember it being posted in POTD and everyone was like "whoa, no way!".
I think a glovebox needs the same thing a SAB does, practice. One can get away with a lot of shit once he gets used to using whatever it is he uses. I would recommend flaming outside the box though. The guy that posted about using a GB in POTD drilled a small hole in the corner of his box so he could flame his scalpel outside the box without actually taking his hands and tools out of it.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Supalemonhaze said: The guy that posted about using a GB in POTD drilled a small hole in the corner of his box so he could flame his scalpel outside the box without actually taking his hands and tools out of it.
That was pretty clever, but it also makes that whole, gloves moving around drawing in currents thing, a lot more of a hazard. Did that guy have his gloves attached?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: glovebox: build your own easily [Re: Inocuole]
#23552100 - 08/18/16 02:18 AM (7 years, 5 months ago) |
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Yeah, he did. If it was any other thread than POTD, I would attempt to fish it out. Finding a post in that thread is worse than a needle in a haystack.
Edit:
Gotta love the search engine.
Quote:
PinPornProducer said:
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GreenRabbit said:
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dankington said:
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PinPornProducer said: I do all my agar in a box, mine has gloves attached though so I can't speak for an sab but I'm pretty sure many growers use them. I know for a fact that my glove box works.   
Even has a little flame port to stick the syringe/scalpel out into the flame and a magnifying glass attached to the lid for agar work lol
Though I don't like having gloves attached myself, this is a dope ass box. I love the magnifying glass! And the flame port.
I didn't notice either. The magnifyng glass is a great idea, I'm gonna have to steal that. I need a bigger SAB and already have a spare lens. If I can find it.
I don't like the flame port. A torch isn't sterilizing all of the air it contacts, and it pushes a lot of air. I would expect flaming through that hole to push in a lot of open air.
I mean, why bother having gloves on it if you are going to have a hole in the tub anyway?
Most of my work is g2g and the box I sealed up during those. I don't have a lab, I do all my work on the coffee table in my living room with a dog and cat running around. I'd do agar in an sab but I'm not going to re attatch gloves everyrime I g2g. It works and they make me feel safe lol 
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: glovebox: build your own easily [Re: Inocuole]
#23552394 - 08/18/16 06:46 AM (7 years, 5 months ago) |
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Quote:
Inocuole said: Ron, I'd like to hear more about this, but I'd also like to share some of the more "ghetto" ways that we've been growing here for some contrast.
Most people are using still air boxes recently. They operate on the concept of still air vs a contaminant free environment. By letting all the contaminants fall to the floor and stick to the walls of the SAB, you can be sure that nothing will fall into your work when you briefly open a jar or agar plate, even if the box itself isn't totally clean.
My SAB, which works great for me, is a modified (not by much) version of this one.
http://www.shroomery.org/forums/showflat.php/Number/20048771#20048771
I get pretty good results with agar and grain by using it.
  
I'm interested in the idea of a glove box that actually works, but lately the consensus has been that the possibility of your box creating drafts which could bring contamination into your work when you move the hands/gloves around is high enough to negate the benefit you'd otherwise receive by having it completely closed off to open air. There's also a lot of talk of flames inside the box creating further updrafts which can cause contamination to ride its way into your work. I have not tested these theories because they appear sound to me and I get great results the way I do now. But I'm interested in some examples of a design that doesn't run into these issues or has a way around them. Is the entire inside of the box sterile? How can this be accomplished if so? During work with agar and grain I never do much cleaning of my box or my surroundings, since the still air negates the need for this, so this is a completely different approach.
I wouldn't recommend inoculating in a bathroom, that's the dirtiest room of the house.
dear inoc: i used my glovebox for years and i made it on the design of the one i described. i made it big enough to comfortably work in and i angled the top downward so i could look in easily. i am going to draw a design later or figure out the dimensions for you. this works good. i kept a good ionizer in there that cleans the air. there is no drafts. keep the inside wiped down with bleach. use bleach anywhere you have cultures. also i used to use a speedclave that worked good for petri dishes. use some chipboard for the body of the glovebox as it will be a bit lighter than plywood and paint it inside and out with oil paint. plexiglass is good for the cover. i always had good clean mycelium and i could isolate a good strain withy these procedures.
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Quote:
Rooster Cogburn said: It should blow your mind leech that this how it was done, parecel upmost respect man, people make a glovebox out of sterilite plastic containers now so they are being snarky. It was you guys and this stuff that opened up the doors for us I think it is absolutely amazing that you guys were pumping out mushrooms with learning everything through mail corresponding and actually growing. I love it dude.
dear rooster thanks alot and i appreciate it
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Quote:
Rooster Cogburn said: Great way of approaching that inoc. Hey Ron, i'm interested if and when laminar flow hoods were implemented when you were growing?
alot of stuff you do not need. stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?
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theGODSmademedoit

Registered: 03/04/16
Posts: 516
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.
Edited by theGODSmademedoit (08/18/16 06:59 AM)
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theGODSmademedoit

Registered: 03/04/16
Posts: 516
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Finaly some one here showed up with some real knowledge....now we can all learn to grow mushrooms.... I love glovebox teks especially this one.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
paracelsusgold said: stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?

I find it a little funny that you feel that way. What do you think of stamets overall?
I've never used a flowhood myself, but some people do seem to be able to build them for a couple hundred dollars. A huge investment, but it surely is one if you're doing a lot of myco work.
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Peteyboy
SpaceWalker



Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 5 months
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Quote:
paracelsusgold said:
Quote:
Rooster Cogburn said: Great way of approaching that inoc. Hey Ron, i'm interested if and when laminar flow hoods were implemented when you were growing?
alot of stuff you do not need. stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?
Wow....your like the Muhammad Ali of mycology...
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: glovebox: build your own easily [Re: Inocuole]
#23552530 - 08/18/16 07:55 AM (7 years, 5 months ago) |
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Quote:
Inocuole said:
Quote:
paracelsusgold said: stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?

I find it a little funny that you feel that way. What do you think of stamets overall?
I've never used a flowhood myself, but some people do seem to be able to build them for a couple hundred dollars. A huge investment, but it surely is one if you're doing a lot of myco work.
Quote:
Peteyboy said:
Quote:
paracelsusgold said:
Quote:
Rooster Cogburn said: Great way of approaching that inoc. Hey Ron, i'm interested if and when laminar flow hoods were implemented when you were growing?
alot of stuff you do not need. stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?
Wow....your like the Muhammad Ali of mycology...
Quote:
Inocuole said:
Quote:
paracelsusgold said: stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?

I find it a little funny that you feel that way. What do you think of stamets overall?
I've never used a flowhood myself, but some people do seem to be able to build them for a couple hundred dollars. A huge investment, but it surely is one if you're doing a lot of myco work.
inoc i think stamets is an opportunist that apparently has made some bucks with his books and his products and i know that he knows alot and i have his one book and have used it in my past mycological work as well. when he first started selling mycological supplies there was only him and another fellow who wrote another book i have two copies of and peele for supplies and prints usually the amazonian. i can tell you that i bought some of his products and i remember i paid him over $100 i think for a humidifying system that was a piece of junk and i threw it away and when i asked for my money back he would not give it back to me and i criticized him heavily as a result and he got pissed off at me and my impression overall was that he is an asshole. what do you need the flowhood for may i ask?
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paracelsusgold

Registered: 08/15/16
Posts: 173
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Re: glovebox: build your own easily [Re: Inocuole]
#23552553 - 08/18/16 08:08 AM (7 years, 5 months ago) |
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Quote:
paracelsusgold said:
Quote:
Inocuole said:
Quote:
paracelsusgold said: stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?

I find it a little funny that you feel that way. What do you think of stamets overall?
I've never used a flowhood myself, but some people do seem to be able to build them for a couple hundred dollars. A huge investment, but it surely is one if you're doing a lot of myco work.
Quote:
Peteyboy said:
Quote:
paracelsusgold said:
Quote:
Rooster Cogburn said: Great way of approaching that inoc. Hey Ron, i'm interested if and when laminar flow hoods were implemented when you were growing?
alot of stuff you do not need. stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?
Wow....your like the Muhammad Ali of mycology...
Quote:
Inocuole said:
Quote:
paracelsusgold said: stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?

I find it a little funny that you feel that way. What do you think of stamets overall?
I've never used a flowhood myself, but some people do seem to be able to build them for a couple hundred dollars. A huge investment, but it surely is one if you're doing a lot of myco work.
inoc i think stamets is an opportunist that apparently has made some bucks with his books and his products and i know that he knows alot and i have his one book and have used it in my past mycological work as well. when he first started selling mycological supplies there was only him and another fellow who wrote another book i have two copies of and peele for supplies and prints usually the amazonian. i can tell you that i bought some of his products and i remember i paid him over $100 i think for a humidifying system that was a piece of junk and i threw it away and when i asked for my money back he would not give it back to me and i criticized him heavily as a result and he got pissed off at me and my impression overall was that he is an asshole. what do you need the flowhood for may i ask?
Quote:
Inocuole said:
Quote:
paracelsusgold said: stamets used to sell stuff that didnt work and was expensive and when i told him he got pissed off at me. why do you need the flowhood? how much is one?

I find it a little funny that you feel that way. What do you think of stamets overall?
I've never used a flowhood myself, but some people do seem to be able to build them for a couple hundred dollars. A huge investment, but it surely is one if you're doing a lot of myco work.
i put two pictures of myself in my galleries
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