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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Beginner Question: WBS jar creation
#23551357 - 08/17/16 09:16 PM (7 years, 5 months ago) |
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Ok so I just attempted to create my first set of WBS jars. I was following a tek off different site which recommend just adding water with the bird seed and PC. I did this then knock up jars I'm on day 8 with no growth not even a sign in the jars and they smell really weird which is a sign I probably screw up some where. I began reading and seen that people soak then drain WBS along with soaking it is this necessary? Some even included simmering would anyone experience recommend this extra step? Also I realize sterilization is very key which I emphasized to the point of wearing dust suit which by the way is strange in home kitchen lol. But there only other place I figure I could of made mistake on jar lid as I used siclone which does allow gas exchange is that key component nt when inoculation of the jars this upcoming trial going use the poly fil like recommended jus had trouble get in lid. Thanks for advice new hobby just looking for pointers and a general direction to get good yeilds. P.S. Is it bad sign to have brown liquid and black seed after PC over PCing?
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Check out foomans WBS tek or mine in my signature. Best to rinse them
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Beginner Question: WBS jar creation [Re: liloldme]
#23551393 - 08/17/16 09:29 PM (7 years, 5 months ago) |
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Also jars were kept in 76 F.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Why do all these damn threads have hearts?
-------------------- KSSS And PE WBS.
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Beginner Question: WBS jar creation [Re: Moabfighter]
#23551409 - 08/17/16 09:36 PM (7 years, 5 months ago) |
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Because that's the image you have chosen for the "post icon"
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morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
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Re: Beginner Question: WBS jar creation [Re: Moabfighter]
#23551441 - 08/17/16 09:49 PM (7 years, 5 months ago) |
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Quote:
Moabfighter said: Why do all these damn threads have hearts?
I heard it was system wide for a user/mod leaving. Not sure who/why.
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Rooster Cogburn
Ranger

Registered: 07/19/16
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Re: Beginner Question: WBS jar creation [Re: liloldme]
#23551473 - 08/17/16 10:00 PM (7 years, 5 months ago) |
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How did you inoculate your jars, you mentioned standing in a dust suit in your kitchen? This is more than likely a factor as well as you jars being sterilized, you also mentioned using silicone for GE, that's usually utilized as a sort of port for sticking a needle into, a filter is the thing you need, polyfil works great, if your having trouble getting it into the hole use a pen or something to help you start guiding it through, it has to be pretty tight. And as someone previously said you can look at one of those teks, I recommend a 12-15 hour soak with bird seed followed by draining and loading into jars, the soak helps germinate the endospores and hydrate the grains a bit befor the cycle. A cheaper and more readily available source of spawn for the future would be just straight millet, milo, or oats from the feed stores which in the end is easier to dial in with the soaking method. Good luck
Edited by Rooster Cogburn (08/17/16 10:01 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Beginner Question: WBS jar creation [Re: morty422]
#23551561 - 08/17/16 10:25 PM (7 years, 5 months ago) |
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Quote:
morty422 said:
Quote:
Moabfighter said: Why do all these damn threads have hearts?
I heard it was system wide for a user/mod leaving. Not sure who/why.
Not left....passed on
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Beginner Question: WBS jar creation [Re: cronicr]
#23551608 - 08/17/16 10:39 PM (7 years, 5 months ago) |
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Quote:
cronicr said:
Quote:
morty422 said:
Quote:
Moabfighter said: Why do all these damn threads have hearts?
I heard it was system wide for a user/mod leaving. Not sure who/why.
Not left....passed on:(
Who?? What??
-------------------- KSSS And PE WBS.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Beginner Question: WBS jar creation [Re: Moabfighter]
#23551846 - 08/17/16 11:57 PM (7 years, 5 months ago) |
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Yes we lost acidic_sloth. Brain tumor that was just found a couple days ago.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Beginner Question: WBS jar creation [Re: cronicr]
#23553032 - 08/18/16 11:26 AM (7 years, 5 months ago) |
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Well i used spore syringe but in future look to use LC as it looks most cost effective. But I see you mention millet or rye I've heard of do these trump WBS?
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sandy_vag
Keep Dreaming



Registered: 06/14/16
Posts: 112
Last seen: 5 years, 8 months
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WBS typically consists of Millet + Milo (red sorghum grain) + Sunflower Seeds, and sometimes corn and wheat gets added too.
I really like straight milo it's Very cheap and works great. Rye is good but much more expensive and seasonal around here. You can't even buy rye half the year locally. Milo is ~$8/50lb sack and always at the feed stores.
Don't do that no prep tek anymore. At the very least soak it for 8-12 hours starting with boiling water. Best to rinse it first.
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Beginner Question: WBS jar creation [Re: sandy_vag]
#23553216 - 08/18/16 12:33 PM (7 years, 5 months ago) |
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I put the WBS in a bucket, pour boiling water into it and let it sit for one hour before rinsing, straining, drying a bit and PC for 2 hours
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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K I will try rye or milo do you still use the rinse and boiling steps with these substrates. Also curious I lifted my container lid today and horrible smell came out is this unusal and there's no growth do I trash jars?
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oontribe


Registered: 01/14/15
Posts: 3,570
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Re: Beginner Question: WBS jar creation [Re: cronicr]
#23553621 - 08/18/16 03:05 PM (7 years, 5 months ago) |
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You should follow the teks here to the t man.
You should build a SAB too.
I think foomans tek is the best when it comes to wbs/millet.
Use polyfill as a filter, its the easiest fastest way to make filters.
Quote:
cronicr said: Yes we lost acidic_sloth. Brain tumor that was just found a couple days ago.
In a better place now...hopefully.
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sandy_vag
Keep Dreaming



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Re: Beginner Question: WBS jar creation [Re: oontribe]
#23553635 - 08/18/16 03:08 PM (7 years, 5 months ago) |
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Horrible smell = bacteria, trash it. What is your lid setup, how long did you PC?
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: Beginner Question: WBS jar creation [Re: sandy_vag]
#23553885 - 08/18/16 04:22 PM (7 years, 5 months ago) |
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Quote:
sandy_vag said: Horrible smell = bacteria, trash it. What is your lid setup, how long did you PC?
Where did your spores come from?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
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Re: Beginner Question: WBS jar creation [Re: JacobStorm]
#23553921 - 08/18/16 04:32 PM (7 years, 5 months ago) |
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Quote:
JacobStorm said:
Quote:
sandy_vag said: Horrible smell = bacteria, trash it. What is your lid setup, how long did you PC?
Where did your spores come from?
wut
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: Beginner Question: WBS jar creation [Re: ComebackKid]
#23553960 - 08/18/16 04:43 PM (7 years, 5 months ago) |
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I'm wondering if he got a syringe from a friend? A vender? could " where did your pores come from" not be more clear?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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ComebackKid
Multispore Enthusiast



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Re: Beginner Question: WBS jar creation [Re: JacobStorm]
#23554061 - 08/18/16 05:09 PM (7 years, 5 months ago) |
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I apologize. I originally posted "lol wut" thinking this was a thread from earlier where OP hadnt even made it to inoculation. Then I edited to just "wut" in hopes of not coming across as a stupid idiot to no avail
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Spores from treasure coast strain. I got 2 ccs left so I am going use the rest of my WBS and try a G2g transfer. Wondering what SAB and my lids are tyrevk with silicon seal doesn't allow for any GE so I figure that's flawed going make lids polyfil hope to get yeild from this strain looking to make LC. Any other pointers be great.
Edited by PiptheGreAtest (09/11/16 01:33 AM)
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Rooster Cogburn
Ranger

Registered: 07/19/16
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Last seen: 7 years, 2 months
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Take the rest of your spores to agar before you do anything else with them, DO NOT make LC or grain with the rest.
Edited by Rooster Cogburn (08/18/16 05:51 PM)
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sandy_vag
Keep Dreaming



Registered: 06/14/16
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Last seen: 5 years, 8 months
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Dude doesn't need to be doing any agar work, he's obviously not there yet. He SHOULD be shooting the spores he has have left into pf jars. I doubt OP would be able to successfully utilize grains to their potential at this time, even if his grain did work. Do a few PF grows and study and learn while you get that down.
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Rooster Cogburn
Ranger

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Re: Beginner Question: WBS jar creation [Re: sandy_vag]
#23554416 - 08/18/16 06:47 PM (7 years, 5 months ago) |
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Quote:
sandy_vag said: Dude doesn't need to be doing any agar work, he's obviously not there yet. He SHOULD be shooting the spores he has have left into pf jars. I doubt OP would be able to successfully utilize grains to their potential at this time, even if his grain did work. Do a few PF grows and study and learn while you get that down.
Wrong. He could very easily utilize agar and save himself from buying another syringe. Go to bohdies agar page https://www.shroomery.org/forums/showflat.php/Number/21922023
or pasties.
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ItalianKillbasa
Stranger


Registered: 03/03/16
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Quote:
Rooster Cogburn said:
Quote:
sandy_vag said: Dude doesn't need to be doing any agar work, he's obviously not there yet. He SHOULD be shooting the spores he has have left into pf jars. I doubt OP would be able to successfully utilize grains to their potential at this time, even if his grain did work. Do a few PF grows and study and learn while you get that down.
Wrong. He could very easily utilize agar and save himself from buying another syringe. Go to bohdies agar page https://www.shroomery.org/forums/showflat.php/Number/21922023
or pasties.
I second this opinion...I'm a super noob and just transitioned to Agar for the first time using Pasty's Plates...they are dummy proof! Plus if done correctly he can definitely rule out if he has a tainted syringe and clean it up before dumping the rest into a PF Tek jar and resulting in contams off the rip!!
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Beginner Question: WBS jar creation [Re: sandy_vag]
#23557481 - 08/19/16 04:50 PM (7 years, 5 months ago) |
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Quote:
sandy_vag said: Dude doesn't need to be doing any agar work, he's obviously not there yet. He SHOULD be shooting the spores he has have left into pf jars. I doubt OP would be able to successfully utilize grains to their potential at this time, even if his grain did work. Do a few PF grows and study and learn while you get that down.
 Lol what? You cant be serious...
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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I'll look into agar and PF TEKS but I really just wanting to get one good jar even if it requires couple of trails I have a friend who been doing it for awhile seeking some his advice along with any info I find on here. Quick question what if you PC the jars without aluminum foil or coffee filters doesn't affect the jars does it?
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sandy_vag
Keep Dreaming



Registered: 06/14/16
Posts: 112
Last seen: 5 years, 8 months
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No need for any coffee filters. Without aluminum foil your polyfil will get wet and your jars could dry out from the steam evaporation while it cools. Wet polyfil (or tyvek or whatever you are using) is a contamination vector inviting contams inside.
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Quote:
PiptheGreAtest said: Spores from treasure coast strain. I got 2 ccs left so I am going use the rest of my WBS and try a G2g transfer. Wondering what SAB and my lids are tyrevk with silicon seal doesn't allow for any GE so I figure that's flawed going make lids polyfil hope to get yeild from this strain looking to make LC. Any other pointers be great.
Do us all a favor and edit out your vender name from your post. Lets not include others in our felonies.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
Edited by JacobStorm (08/21/16 03:41 PM)
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: Beginner Question: WBS jar creation [Re: sandy_vag] 1
#23560570 - 08/20/16 05:08 PM (7 years, 5 months ago) |
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Quote:
sandy_vag said: Dude doesn't need to be doing any agar work, he's obviously not there yet. He SHOULD be shooting the spores he has have left into pf jars. I doubt OP would be able to successfully utilize grains to their potential at this time, even if his grain did work. Do a few PF grows and study and learn while you get that down.
Wait whats wrong with this suggestion? Like syringes are hella expensive? what 10 bucks or something? PF tek is a great place to start. Agar brings on other needed supplies that most don't already have laying around. Yes OBVIOUSLY agar is awesome for a lot of reasons but that doesn't mean its the OP's best or only option right now.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Beginner Question: WBS jar creation [Re: JacobStorm]
#23560661 - 08/20/16 05:41 PM (7 years, 5 months ago) |
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Yo jacob youre gunna have to edit your post as well Abd youre right agar is not the only option. But youre also wrong because it IS the best option
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Beginner Question: WBS jar creation [Re: ComebackKid]
#23563133 - 08/21/16 02:22 PM (7 years, 5 months ago) |
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Sooty for mistake took out name but I started another jar of Ecudor strain in WBS if these fail will switch to rye berries of milo or watever I'm really just trying to concentrate on bulk grow as that is how my mentor grows just looking for pointers so I'm not always just tagging along. Hopefully these take I also heard that the jars should be keep in ambient light is this ture as the tek said in dark place?
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: Beginner Question: WBS jar creation [Re: ComebackKid]
#23563294 - 08/21/16 03:43 PM (7 years, 5 months ago) |
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Quote:
ComebackKid said: Yo jacob youre gunna have to edit your post as well
Thanks for catching that!
Why is it the best option?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Quote:
PiptheGreAtest said: Sooty for mistake took out name but I started another jar of Ecudor strain in WBS if these fail will switch to rye berries of milo or watever I'm really just trying to concentrate on bulk grow as that is how my mentor grows just looking for pointers so I'm not always just tagging along. Hopefully these take I also heard that the jars should be keep in ambient light is this ture as the tek said in dark place?
If your tek says the dark is for colonizing you have a out dated tek mate. Yes light is good for the mycelium. What tek are you following?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Beginner Question: WBS jar creation [Re: JacobStorm]
#23585567 - 08/27/16 11:36 PM (7 years, 5 months ago) |
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Kungpow bulk grow lil dated but I can't even get anything to colonize in jar I'm going try mil switch to my bath room more sanitary.
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7munkee
Berilion



Registered: 08/10/15
Posts: 273
Loc: North east US
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Re: Beginner Question: WBS jar creation [Re: PiptheGreAtest] 1
#23585866 - 08/28/16 05:05 AM (7 years, 5 months ago) |
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My first grow was PF. Out of 6 cakes I had one contam and got a grand total of 5 grams dry. My next 3 grows were WBS in a 40 qt mono and I got an average of 3 ounces each. I am now playing with agar(unsuccessfully). Go one step at a time for the learning experience. You will get the most benefit from it.
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Rooster Cogburn
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Build a still air box, the bathroom is one of the dirtiest places in the house, you will never get anywhere until you spend 7 bucks on a plastic box to do your inoculations in.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Got a still air box and milo trying new substrate hope this one works trying Ecudor strain.
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Rooster Cogburn
Ranger

Registered: 07/19/16
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Last seen: 7 years, 2 months
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I grow EQ from milo spawn Good luck dude and don't be afraid to jump into the agar and get yourself a nice EQ culture in the future. What new substrate are you using?
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Quote:
Rooster Cogburn said: Build a still air box, the bathroom is one of the dirtiest places in the house, you will never get anywhere until you spend 7 bucks on a plastic box to do your inoculations in.
I do open air inoculation in my master bathroom. Havent had a problem since its well cleaned1
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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Rooster Cogburn
Ranger

Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
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Re: Beginner Question: WBS jar creation [Re: JacobStorm]
#23636267 - 09/11/16 04:15 PM (7 years, 4 months ago) |
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Quote:
JacobStorm said:
Quote:
Rooster Cogburn said: Build a still air box, the bathroom is one of the dirtiest places in the house, you will never get anywhere until you spend 7 bucks on a plastic box to do your inoculations in.
I do open air inoculation in my master bathroom. Havent had a problem since its well cleaned1
"The highway is for gamblers, better use your sense" -Bob Dylan
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Quote:
Rooster Cogburn said:
Quote:
JacobStorm said:
Quote:
Rooster Cogburn said: Build a still air box, the bathroom is one of the dirtiest places in the house, you will never get anywhere until you spend 7 bucks on a plastic box to do your inoculations in.
I do open air inoculation in my master bathroom. Havent had a problem since its well cleaned1
"The highway is for gamblers, better use your sense" -Bob Dylan
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Well I got 6 colonize jars now. I am ready to transfer to bulk substrate. I had a quick question I was talking sum one they told me once I get the bulk substrate colonized I'm able to like pull a core from it and place in another monotube to generate 2 tubs from 1 is this a true statement?
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X2QE
:(){ :|:& };:

Registered: 07/17/16
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No, it is not a true statement. Don't do this. I see you asked this question already.
Please look up Grain to Grain (G2G) transfer.
Do the transfer in a Still Air Box (SAB)
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Quote:
PiptheGreAtest said: Well I got 6 colonize jars now. I am ready to transfer to bulk substrate. I had a quick question I was talking sum one they told me once I get the bulk substrate colonized I'm able to like pull a core from it and place in another monotube to generate 2 tubs from 1 is this a true statement?
Could I get a link to this conversation? Never heard of it although cant deny or confirm it. Sounds like BS to me.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Quote:
PiptheGreAtest said: Well I got 6 colonize jars now. I am ready to transfer to bulk substrate. I had a quick question I was talking sum one they told me once I get the bulk substrate colonized I'm able to like pull a core from it and place in another monotube to generate 2 tubs from 1 is this a true statement?

"Cores" from one tub to another??!! Terribly ridiculous...
The grains that you bring to the table when you spawn a tub has a direct relationship with the bio-efficiency of said tub...
Taking a "core" of one tub and bringing it to another is an awesome way to get zero to no results from the next.
Check my signature and look at the threads inside the agar portal. That is the best way to continue a culture. There's a reason why all of the members of this forum use agar to propagate their grows...
Cores... sorry man-your friend is remedial....

Let us know any other questions you have!
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ComebackKid
Multispore Enthusiast



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Re: Beginner Question: WBS jar creation [Re: morty422]
#23760783 - 10/22/16 12:12 PM (7 years, 3 months ago) |
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You know what. I was thinking about the whole core idea for a while. But after Morty putting it like that it really makes no sense.
Besides the obvious contam potential, Spent sub being used to colonize sub with zero nutrients in it makes no sense at all.
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Tmoney69
Stranger

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Re: Beginner Question: WBS jar creation [Re: X2QE]
#23760815 - 10/22/16 12:25 PM (7 years, 3 months ago) |
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I agree with grain 2 grain. I'm a noob aswell and didn't wanna buy expensive syringes. So I got a few jars from a friend and from what I hear if done correctly it's endless supply. One question about g2g for anybody else reading this n knows, but I hear g2g is kinda like cloning? I was told that any tub I did from that kind would pretty much be the same? And maybe the person you was having a conversation with was actually talking bout g2g and not taking a core from a tub. Cause I was confused at first thinking long as it was myc something would happen.
Edited by Tmoney69 (10/22/16 12:27 PM)
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