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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: c10h12n2o]
#23608204 - 09/03/16 02:27 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 09:33 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: amidogen]
#23608248 - 09/03/16 02:37 PM (7 years, 4 months ago) |
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Well once a kind fellow pointed out that we're already doing said idea every time we grow, that did shoot down a lot of the potential for conversation on the possibilities.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Inocuole]
#23608283 - 09/03/16 02:47 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 09:34 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Inocuole]
#23608286 - 09/03/16 02:48 PM (7 years, 4 months ago) |
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again, that underscores why this is so interesting, and further proves that the concept is prevalent throughout all of our projects whether or not we talk about it.
that is what led me to initially wonder about these topics. i am frankly surprised that so many people didnt realize that this is happening every time we grow. i dont see how someone could even follow the conversation if they didnt understand that.
really goes to show how much people are missing the point and how little people are actually thinking about the topics presented
the thread has certainly given me some fun things to look into, and i doubt i am alone in that
particularly pasty's posts on the metabolism of various contams and how they differ, and how they affect the medium composition
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (09/03/16 02:53 PM)
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: c10h12n2o]
#23608336 - 09/03/16 03:02 PM (7 years, 4 months ago) |
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No one was missing the point. I'd say a more damning fact was simply overlooked by overthinking. But I'm quite certain everyone in the discussion understood the basic premise of introducing dead contams/endospores.
Everyone here knows how vaccines and immune systems work. We also understand the cause/effect of metabolites. So, what's with this circular argument?
Edited by dankington (09/03/16 03:03 PM)
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: dankington]
#23608352 - 09/03/16 03:06 PM (7 years, 4 months ago) |
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Preach
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Loc: the abyss
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: dankington]
#23608368 - 09/03/16 03:10 PM (7 years, 4 months ago) |
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we were talking about a lot more than just that, but i guess you missed that? or are you just focusing on the dumbed-down phrase OP used to try to introduce people to the concept, to the exclusion of what people are actually interested in?
anyway, i would love for anyone capable of getting un-derailed by this nonsense to actually talk about some of the many valid discussion points that have been presented (many enumerated above), share any useful notes they have on the topic, raise any good questions, share any interesting papers/links/photos/projects, etc., to do so
because, if its just people who didnt even make the connection that things like this is already happening all the time trying to convince people who are actually interested in the excretions/metabolites/constituents of microorganisms that they are somehow dumb for even wondering about such things, youre right, no point in that
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: c10h12n2o]
#23608393 - 09/03/16 03:18 PM (7 years, 4 months ago) |
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Quote:
c10h12n2o said: again, that underscores why this is so interesting, and further proves that the concept is prevalent throughout all of our projects whether or not we talk about it.
Well it hasn't made a difference yet, we still have to sterilize and pasteurize.. How many years and generations of growth do you think it might take before it finally matters?
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Inocuole]
#23608420 - 09/03/16 03:30 PM (7 years, 4 months ago) |
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and nothing presented is meant to suggest otherwise
idk how long it would take or if it would even be possible to do what you are talking about (creating a culture that wouldnt require pasteurization, etc)
but i do know that there is some amazing research being done into fungal and bacterial antibiotics/antifungals being produced by a broad range of microorganisms. it is really amazing stuff, with new compounds being isolated and written about every day. and the right mind might be able to apply some of the findings and techniques to our hobby.
and that is truly fascinating, something worth talking about in my opinion
one answer to your question would be zero, if you spawn it outdoors (where lots of competitors are present btw)
but mainly i think it is something interesting to think about and discuss, such as the various talking points i brought up.
and this is the kind of place where someone brilliant might pop in and say something incredibly insightful that gives me a whole new perspective and tons of new things to research ****cue RR****
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (09/03/16 03:31 PM)
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: c10h12n2o]
#23608421 - 09/03/16 03:31 PM (7 years, 4 months ago) |
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I'm not at a keyboard, nor inclined to quote a page of text. I was merely being succinct. Pardon me for I'm obv. intelectually inferior. I do feel i successfully summed everything up, thank you.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: c10h12n2o]
#23608430 - 09/03/16 03:37 PM (7 years, 4 months ago) |
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Quote:
c10h12n2o said: ****cue RR****
Be fucking hilarious if he popped in just to close the thread. That's so his style.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Inocuole]
#23608441 - 09/03/16 03:41 PM (7 years, 4 months ago) |
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I'm amazed that I didn't get thread locked or temporarily banned for this now that you mention it.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
#23608446 - 09/03/16 03:43 PM (7 years, 4 months ago) |
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A ban would be extreme.
I've got more analogies though if you like those. For instance, would eating enough salad vaccinate me against lettuce? If I eat shark all the time, maybe they'll leave me alone in the ocean? No? Alright, you all have a good one.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Inocuole]
#23608454 - 09/03/16 03:46 PM (7 years, 4 months ago) |
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Both of my temporary bans were over trivial BS, its all up to the mod present, IME anyway.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr] 1
#23608511 - 09/03/16 04:06 PM (7 years, 4 months ago) |
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again, completely ignoring all the great talking points raised, and focusing on what we all agree is bs... why?
if you cant figure out what we are talking about, or have nothing useful to add, what is the point?
i think inocuole is just trolling by focusing on nonsense posts rather than connecting the dots or addressing any of the valid talking points :/ he knows good and well that the phrase "vaccinate" was used to try to introduce the subject to those who might not realize that what we are actually dealing with is the metabolites/constituents of microorganisms, the effects they have on our projects, and the potential that mediums enhanced in this particular way might have within our hobby. either trolling or i vastly overestimated his ability to connect the dots / research skills, or underestimated how much he enjoys the whole "big dog" / "underdog" dynamic
all that said, the topic is definitely better suited to advanced... probably would have been most productive to just PM pasty (like i will when i have some questions about how some papers might relate to our hobby), but then users like me and others who read more than we post wouldnt have even seen it
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: c10h12n2o]
#23608527 - 09/03/16 04:09 PM (7 years, 4 months ago) |
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I did chuckle to myself when I got a tiny spot of green in an otherwise clean grain jar the other day. Laughing about the "lion in my rabbit cage" who appeared to be having difficulty eating all the rabbits for some reason.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: c10h12n2o]
#23608562 - 09/03/16 04:19 PM (7 years, 4 months ago) |
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Being bombastic does not make one superior or even correct. In fact, it sounds to me like you're grasping at straws to save face now. The dots were connected fine.
Perhaps it is you that is misunderstanding?
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: Psilosopherr]
#23608564 - 09/03/16 04:19 PM (7 years, 4 months ago) |
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Holy shit this threads gone mad I read it when I t started not paid much attention for a while and its 8 pages deep Jizz in it maybe the jizz will eat contams and grow half human half shrooms resulting in shrumans
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: mrmazdarx9]
#23608632 - 09/03/16 04:39 PM (7 years, 4 months ago) |
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Quote:
mrmazdarx9 said: resulting in shrumans
And to think someone suggested this conversation wasn't going anywhere productive..
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Vaccinating clean mushroom cultures with dead/attenuated contaminants [Re: mrmazdarx9]
#23608644 - 09/03/16 04:42 PM (7 years, 4 months ago) |
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i dont even see anything to respond to, just more ego bs
anyone who actually has anything valid to say about any of the MANY valid talking points that have been brought up (i enumerated many in my post), any info to share, etc., it is very welcome, and im willing to bet i am not the only one who appreciates it
all this bs is doing is making it clear who is NOT going to contribute anything helpful (which would have been better served by silence)
writing off entire avenues of research is just naive and arrogant, especially considering that identifying useful compounds from microorganisms has been one of the most productive lines of reasoning in modern medical history. exceedingly arrogant and naive
for the nth time, i invite anyone to actually discuss and share notes about the talking points presented, specifically the ones in my posts, if you can find them between the attempts to derail anyone's meaningful conversation
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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